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Zhang Hongyuan, Liu Kewu, Jiang Yun, Gong Youbin, Luo Shengqing. FUNCTIONAL GROUPS OF ALKALINE PHOSPHATASE FROM ANODONTA WOODIANA HEUDE[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 347-352.
Citation: Zhang Hongyuan, Liu Kewu, Jiang Yun, Gong Youbin, Luo Shengqing. FUNCTIONAL GROUPS OF ALKALINE PHOSPHATASE FROM ANODONTA WOODIANA HEUDE[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 347-352.
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FUNCTIONAL GROUPS OF ALKALINE PHOSPHATASE FROM ANODONTA WOODIANA HEUDE

  • Department of Biology, Sichuan University, Chengdu, 610064

  • Received Date: July 10, 1994
  • Rev Recd Date: July 15, 1997
  • Published Date: October 24, 1997
    Graphical Abstract
    • Abstract
  • The alkaline phosphatase from Anodonta woodiana Heude was selectively modified by PMSF DTT, PCMB, NBS, TNBS, SUAN BrAc and IAc, and changes in the activities of the enzyne have been detected. The reachon of AKP with PMSF NBS,TNBS, SUAN and DTT resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration. The bromoacetic acid, iodoacetic acid and p-chloromercuri-benzoic acid were found without any inhibition effect Studies of inactivation of AKP by chemical modification demostrated that Ser, Lys and Trp residues are indirpensble functional groups of the AKP. Partial disulfide bond are also for the function of this enzyme.
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