Volume 30 Issue 6
Aug.  2014
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WEI Wei, LIANG Cheng-Wei, QIN Song. FUNCTIONAL ANALYSIS OF THE PROMOTER OF BKT ENCODING BETA-CAROTENE KETOLASE IN HAEMATOCOCCUS PLUVIALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(6): 747-751.
Citation: WEI Wei, LIANG Cheng-Wei, QIN Song. FUNCTIONAL ANALYSIS OF THE PROMOTER OF BKT ENCODING BETA-CAROTENE KETOLASE IN HAEMATOCOCCUS PLUVIALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(6): 747-751.
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FUNCTIONAL ANALYSIS OF THE PROMOTER OF BKT ENCODING BETA-CAROTENE KETOLASE IN HAEMATOCOCCUS PLUVIALIS

  • Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;
    2. Graduate school, Chinese Academy of sciences, Beijing, 100039

Funds: 

the National Natural Science Foundationof China (No.30470156)andthe KeyInnovative Project of the Chinese Academyof Sciences(No.KZCX3-SW-215)

  • Received Date: December 25, 2005
  • Rev Recd Date: March 08, 2006
  • Published Date: November 24, 2006
    Graphical Abstract
    • Abstract
  • The unicellular green alga Haematococcus pluvialis accumulates a highvaluable astaxanthin under stress conditions. Betacarotene ketolase (BKT), a key enzyme in astaxanthin biosynthesis in H. p luvialis, catalyzes the conversion of B-carotene to canthaxanthin and zeaxanthin to astaxanthin. Electrophoresis mobility shift assay (EMSA) was used in H. pluvialis to identify transcription factor binding sites within a 309 bp promoter region (-617/-309) of betacarotene ketolase gene and a 59 bp sequence between -396 and -338 bp was found to have a specific binding activity to the nuclear protein. Sequence analysis revealed that this important functional region contains neither TATA nor CAAT box but a Gbox involved in the responsiveness of light, anaerobiosis, pcoumaric acid and hormone.
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    • References (15)
  • [1]
    Manjunath S, Sachs M M. Molecular characterizat ion and promot eranalysis of the maize cytosol ic glyceraldehyde 3-phosphate dehydrogenasegene family and its expression during anoxia [J]. Plant Mol ecularBiology, 1997, 33(1) : 97 ) 112
    [2]
    Lotan T, Hirschberg J. Cloning and expression in Escherichia col i ofthe gene encoding beta-C-4-oxygenase, that converts beta-carot ene tothe ketocarotenoid canthaxanthin in Haematococcus pluvialis [J].FEBS letters, 1995, 364: 125
    [3]
    Grnewald K., Hirschberg J., Hagen C. Ketocarotenoid biosynthesisoutside of plastids in the unicellular green alga Haematococcus pluvialis[J]. The Journal of Biological Chemistry, 2001, 276: 6023 ) 6029
    [4]
    Kajiwara S, Kakizono T, Sait o T, et al. Isolation and functionalidentificat ion of a novel cDNA for astaxant in biosynthesis fromHaematococcus pluvialis, and astaxanthin synthesis in Escherichia coli[J]. Plant Molecular Bi ology, 1995, 29: 343 ) 352
    [5]
    Linden H. Carotenoid hydroxylase from Haematococcus pluvialis :cDNA sequen ce, regulat ion and funct ional complement ation [J].Biochimica et Biophysica Acta, 1999, 1446: 203) 212
    [6]
    St einbrenner J, Linden H. Regulation of two carotenoid biosynthesisgenes coding for phyt oene synthase and carotenoid hydroxylase duringstress-induce astaxanthin format ion in the alga Haematococcus pluvialis[J]. Plant Physiology, 2001, 125: 810) 817
    [7]
    Sun Z, Francis X, Cunningham J, Gantt E. Diff erential expressionof two isopent enyl pyrophosphate isomerases and enhanced carotenoidaccumulation in a uni cellular chlorophyt e [J]. Proceedings of NationalAcademy of Sciences USA, 1998, 95: 11482 ) 11488
    [8]
    Grnewald K, Eckert M, Hirschberg J, et al.Phytoene desaturase islocalized exclusively in the chloroplast and up-regulat ed at the mRNAlevel during accumulat ion of secondary carot enoids in Haematococcuspluvialis (Volvlcales, chlorophyceae) [J]. Plant Physi ology, 2000,122: 1261) 1268
    [9]
    Meng C X, Teng C Y, Jiang P, et al. Cloning and characterizat ionof beta-carotene ketolase gene promoter in Haematococcus pluvialis7 50 ACTA HYDROBIOLOGICA SINICA 30卷[J]. Acta Biochimica Bi ophysica Sin, 2005, 37(4) : 270) 275
    [10]
    Lescot M, Dehais P, Thi js G, et al. PlantCARE: a database ofplant cis-act ing regulatory elements and a portal to tools for in sil icoanalysis of promoter sequences [J]. Nucleic Acids Research, 2002,30: 325 ) 327
    [11]
    Rydz S K, Prieto J L, Rychter A M, et al. A DNA-binding activityfor the promoter of the gene encoding C4 phosphoenolpyruvate carboxylaseis modulat ed by phosphorylation during greening of theSorghum leaf [J]. Plant Science, 2000, 159: 65 ) 73
    [12]
    Giuliano G, Pichersky E, Malik V S, et al. An evolutionarily conservedprotein binding sequence upstream of a plant light regulatedgene [J]. Proceedings of National Academy of Sciences USA, 1988,85: 7089 ) 7093
    [13]
    McKendreeW L Jr, Ferl R J. Functional elements of the ArabidopsisAdh promoter include the G-box [J]. Plant Molecular Biol ogy,1992, 19(5) : 859 ) 862
    [14]
    Loake G J,Faktort O,Lamb C J.Combination of H-box [CCTACC(N) 7CT]and G-box (CACGTG) cis elements is necessary for feed-forwardst imulat ion of a chalcone synthase promoter by the phenylpropanoidpathwayintermediate p-coumaric acid [J]. Proceedings of NationalAcademy of Sciences USA, 1992, 89: 9230 ) 9234
    [15]
    Mason H S, DeWald D B, Mullet J E. Ident if ication of a Methyl Jasmonate-Responsive Domain in the Soybean vspB Promot er [J]. ThePlant Cel l, 1993, 5: 241 ) 251
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