LAO Hai-Hua, BAI Jun-Jie, YE Xing, JIAN Qing, LUO Jian-Ren. MOLECULAR CLONING AND ANALYSIS OF TWO DISTINCT GROWTH HORMONE cDNAs FROMCAR ASSIUS AURATUS AURATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 74-79.
Citation: LAO Hai-Hua, BAI Jun-Jie, YE Xing, JIAN Qing, LUO Jian-Ren. MOLECULAR CLONING AND ANALYSIS OF TWO DISTINCT GROWTH HORMONE cDNAs FROMCAR ASSIUS AURATUS AURATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 74-79.

MOLECULAR CLONING AND ANALYSIS OF TWO DISTINCT GROWTH HORMONE cDNAs FROMCAR ASSIUS AURATUS AURATUS

  • Received Date: December 17, 2002
  • Rev Recd Date: March 09, 2003
  • Published Date: January 24, 2004
  • Growth hormone, a 22-kDa single-chain polypeptid e hormone synthesized and secreted by the anterior pituitary gland, plays an impo rtant role in the regulation of growth, development,metabolism and appetite.It is one of the best-studied pituitary hormones that have been used as models for studying of physiology, nutritional composition, regulating of gene expression, structure-function relationships and gene evolution. In mammals,growth hormone variants arising from differential processing of a single primary RNA transcript have been reported, whereas the growth hormone variants found in teleost appear to be the products of different genes.In previous studies, a few teleost fishes h ave two distinct growth hormones, such as chum salmon(Oncorhynchus keta), ch inook salmon (Oncorhynchus tshawytscha), coho salmon(Oncorhynchus kisutch),Atlantic salmon(Salmo salar), rainbow trout (Oncorhynchus mykiss),At lantic cod(Gadus morhua),Japanese eel(Anguilla japonica),goldfish(Car assius auratus),etc.Growth hormone gene duplication was believed to be the re sult of polyploidization among ancestral aquatic vertebrates. There is a close r elationship betweenCarassius auratus auratusand goldfish, belonging to the same family of Cyprinidae.Carassius auratus auratuswere reported to be pol yploid, so it was possible thatCarassius auratus auratusalso had two disti nct growth hormone genes in a single body.Gene duplication was a rare event. Whe ther there were two formsCarassius auratus auratusgrowth hormone cDNAs enc oding distinct growth hormone was still unknown,it was significative to study th e growth hormone polypeptides ofCarassius auratus auratus.
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    Bai J J, Ma J, Jian Q, et al. Cloning of cDNA for common carp GH and its expression in prokaryocyte. Chinese Journal of Biochemistry and Molecular Biology [J].1999,15(3):409-412[白俊杰,马进,简清,等.鲤鱼(Cyprinus carpio)生长激素基因克隆及原核表达[J].中国生物化学与分子生物学报,1999,15(3):409-412][2] Forbes S H,Knudsen K L,North T W, et al.One of two growth hormone genes in coho salmon is sex-linked [J].Proc. Natl.Acad.Sci.USA 1994,91:1628-1631[3] Rand-Weaver M, Noso T, Kawauchi H.Isolation and characterization of somatolactin, a new protein related to growth hormone and prolactin from Atlantic cod(Gadus morhua)pituitary glands [J].Biochemistry,1991,30(6):1509-1515[4] Law S M,Cheng K W, Fung T K, et al.Isolation and characterization of two distinct growth hormone cDNA from the goldfish,Carassius auratus [J]. Arch of Biochem. Biophys, 1996,330(1):19-23[5] Du S J, Delvin R H, Hew C L. Genomic structure of growth hormone gene in chinook salmon (Oncorhynchus tshawytscha):presence of two functional genes, GHⅠ and GHⅡ, and a male-specific pseudogene, GH-psi. DNA [J].Cell Biol, 1993,8:739-751[6] Lemaire C, Warit S, Panyim S. Giant catfish Pangasianodon gigas growth hormone-encoding cDNA cloning and sequencing by one-sided polymerase chain reaction [J].Gene, 1994, 149:271-276[7] Lao H H,Bai J J,Ye X, et al. Molecular clonging and sequence anslysis of growth hormone cDNAs from two species of gurnards [J].Journal of Agricultural Biotechnology,2001,9(4):343-347[劳海华,白俊杰,叶星,等.团头鲂和广东鲂生长激素cDNA的分子克隆和序列分析[J].农业生物技术学报,2001,9(4):343-347][8] Larhammar D, Risinger C. Molecular genetic aspects of tetraploidy in the common carp Cyprinus carpio [J]. Mol. Phylogenet. Evol,1994,3(1):59-68[9] Male R, Nerland A H, Lorens J B,et al.The complete nucleotide sequence of the Atlantic salmon growth hormone I gene [J]. Biochim.Biophys.Acta,1992,1130(3):345-348[10] Buchner J, Rudolph R. Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli [J].Biotechnology, 1991,9(2):157-162[11] Fine M, Sakal E, Vashdi D, et al. Recombinant carp (Cyprinus carpio) growth hormone:expression,purification, and determination of biological activity in vitro and in vivo [J]. Gen. Comp. Endocrinol,1993,89(1):51-61

    Bai J J, Ma J, Jian Q, et al. Cloning of cDNA for common carp GH and its expression in prokaryocyte. Chinese Journal of Biochemistry and Molecular Biology [J].1999,15(3):409-412[白俊杰,马进,简清,等.鲤鱼(Cyprinus carpio)生长激素基因克隆及原核表达[J].中国生物化学与分子生物学报,1999,15(3):409-412][2] Forbes S H,Knudsen K L,North T W, et al.One of two growth hormone genes in coho salmon is sex-linked [J].Proc. Natl.Acad.Sci.USA 1994,91:1628-1631[3] Rand-Weaver M, Noso T, Kawauchi H.Isolation and characterization of somatolactin, a new protein related to growth hormone and prolactin from Atlantic cod(Gadus morhua)pituitary glands [J].Biochemistry,1991,30(6):1509-1515[4] Law S M,Cheng K W, Fung T K, et al.Isolation and characterization of two distinct growth hormone cDNA from the goldfish,Carassius auratus [J]. Arch of Biochem. Biophys, 1996,330(1):19-23[5] Du S J, Delvin R H, Hew C L. Genomic structure of growth hormone gene in chinook salmon (Oncorhynchus tshawytscha):presence of two functional genes, GHⅠ and GHⅡ, and a male-specific pseudogene, GH-psi. DNA [J].Cell Biol, 1993,8:739-751[6] Lemaire C, Warit S, Panyim S. Giant catfish Pangasianodon gigas growth hormone-encoding cDNA cloning and sequencing by one-sided polymerase chain reaction [J].Gene, 1994, 149:271-276[7] Lao H H,Bai J J,Ye X, et al. Molecular clonging and sequence anslysis of growth hormone cDNAs from two species of gurnards [J].Journal of Agricultural Biotechnology,2001,9(4):343-347[劳海华,白俊杰,叶星,等.团头鲂和广东鲂生长激素cDNA的分子克隆和序列分析[J].农业生物技术学报,2001,9(4):343-347][8] Larhammar D, Risinger C. Molecular genetic aspects of tetraploidy in the common carp Cyprinus carpio [J]. Mol. Phylogenet. Evol,1994,3(1):59-68[9] Male R, Nerland A H, Lorens J B,et al.The complete nucleotide sequence of the Atlantic salmon growth hormone I gene [J]. Biochim.Biophys.Acta,1992,1130(3):345-348[10] Buchner J, Rudolph R. Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli [J].Biotechnology, 1991,9(2):157-162[11] Fine M, Sakal E, Vashdi D, et al. Recombinant carp (Cyprinus carpio) growth hormone:expression,purification, and determination of biological activity in vitro and in vivo [J]. Gen. Comp. Endocrinol,1993,89(1):51-61

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