Volume 26 Issue 5
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PANG Yan bin, LI Shu qiang. STUDIES ON THE EFFECT OF CYTOCHALASIN B ON MAJOR CORTICAL CYTOSKELETAL PROTEINS OF TETRAHYMENA THERMOPHILA DURING CONJUGATION[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 501-504.
Citation: PANG Yan bin, LI Shu qiang. STUDIES ON THE EFFECT OF CYTOCHALASIN B ON MAJOR CORTICAL CYTOSKELETAL PROTEINS OF TETRAHYMENA THERMOPHILA DURING CONJUGATION[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 501-504.
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STUDIES ON THE EFFECT OF CYTOCHALASIN B ON MAJOR CORTICAL CYTOSKELETAL PROTEINS OF TETRAHYMENA THERMOPHILA DURING CONJUGATION

  • Biology Department, East China Normal University, Shanghai 200062

  • Received Date: November 19, 2001
  • Rev Recd Date: January 19, 2002
  • Published Date: September 24, 2002
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    • Abstract
  • The effect of cytochalasin B on cortical cytoskeletal proteins of the complementary mating types of Tetrahymena thermophila BF1 and BF5 during their conjugation was carried out, especially during the initial stage of fusion membrane formation stage and the stage of pronuclei exchange. We found that after the treatment of CB, 146KD cortical cytoskeletal protein disappeared, while it existed in the control group, and the percent of 27KD、43KD、47KD and 174KD declined, but the percent of 32KD、41KD、51KD and 54KD kept almost unchanged. Thus we concluded that 27KD、43KD、47KD、146KD and 174KD may be the microfilament proteins.
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    • References (10)
  • [1]
    James J Geyer, John A Kloetzel. Cellular dynamics of conjugation in the ciliate Euplotes aediculatus: 1.cytoskeletal elem ents[J]. Journal of morphology, 1987, 192: 27-42
    [2]
    Vaudaux P E, et al. Cytoskeletal proteins of the cell surface in Te trahymena Ⅱ Turnover of major proteins[J]. Exp. Cell Res.,1979, 1 23: 321-331
    [3]
    Laemmli U K. Cleavage of Structural proteins during the assembly of the head of bacteriophage T4[J]. Nature)lond.), 1970, 277: 680-685
    [4]
    Sambrook J, Fritsch E F and Maniatis T. Molecular cloning a laborat ory Manual 2nd ed [M]. New York: Cold Spring Harbor Laboratory Press, 1989
    [5]
    Kloetzel J A and Sapra G K. Cytochalasin B inhibits separation of c onjugating Euplotes[J]. J. protozool, 1974, 21: 418
    [6]
    Orias E. Ciliate conjugation[M]. Orlando: Academic Press, 1986
    [7]
    Hamilton E P, Orias E, Suhr-Jessen PB. Fertilization failures indu ced by inhibitors of microtubule assembly [J]. J. Cell Biol., 1978, 79, F932
    [8]
    Numata O, Sugai T, and Watanabe Y. Control of germ cell nuclear beh avior at fertilization by Tetrahymena intermediate filament protein [J]. Nat ure)London), 1985, 314: 192-194
    [9]
    Orias J, Hamilton E, Orias E. A microtubule meshwork associated wit h gametic pronucleustransfer across a cell-cell junction [J]. Science, 19 83, 222: 181-183
    [10]
    Li Shuqiang, Pang Yanbin. Studies on the influence of Colchicine and CB on the major cortical cytoskeletal proteins of Tetrahymena thermophila at th e late stage of Conjugation[J]. Acta Hydrobiologica Sinica, Submitted.
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