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CHEN Si-Jia, YANG Fang, ZHENG Wen-Jie, BAI Yan, HUANG Zhi, ZHOU Yan-Hui. EFFECTS OF SELENIUM STRESS ON ANTIOXIDASE SYSTEM OF SPIRULINA PLATENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(5): 706-711.
Citation: CHEN Si-Jia, YANG Fang, ZHENG Wen-Jie, BAI Yan, HUANG Zhi, ZHOU Yan-Hui. EFFECTS OF SELENIUM STRESS ON ANTIOXIDASE SYSTEM OF SPIRULINA PLATENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(5): 706-711.
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EFFECTS OF SELENIUM STRESS ON ANTIOXIDASE SYSTEM OF SPIRULINA PLATENSIS

  • Department of Chemistry, Jinan University, Guangzhou 510632, China;
    2. Insititute of Hydrobiology, Jinan University, Guangzhou510632, China;
    3. Department of Biology, Jinan University, Guangzhou 510632, China

  • Received Date: September 22, 2005
  • Rev Recd Date: December 24, 2006
  • Published Date: September 24, 2007
    Graphical Abstract
    • Abstract
  • Selenium (Se) is an essential element for antioxidation reactions in human and animals. It acted as an antioxidant at low concentrations, but as a pro-oxidant at higher ones, consequently it exerted dual effects on organisms. Previous works have shown that Spirulina platensis (S. platensis) possesses a good tolerance to high levels of Se and it is a good carrier for Se biotransformation. However, there is less information on Seinduced oxidative stress and antioxidant systems in S. platensis. In this paper, we investigated the effects of Se stress on the antioxidase system and lipid peroxidization of S. platensis. Therewere five test sets in the experiment. Se was added in the logarithm growth time of S. platensis from the 5thto 10thday respectively. The accumulative concentrations of Se in test sets are all 1000 mg/ L, but the time of Se addition was different. The results showed that the activities of antioxidase in S. platensis under Se stress were higher than those in the control group or as high as them. GPX changed enormously. The activity of GPX in test set 褀as 2015 times as high as control, and then dropped with the decrease of Se stress intensity. The trend of SOD was similar to that of GPX, but its response to Se stress was not sensitive. POD, CAT and APX increased with the drop of Se stress intensity. It was only in the experimental group 裻hat the content of MDA was higher than that in the control group. The ability of S. platensis to scavenge hydroxy radical had a negative correlation with the MDA content. Their changes indicated the dual effects of Se on the antioxidant system of S. platensis. The concept of Se stress intensity can give a good explanation to the experimental results.
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    • References (15)
  • [1]
    Hartikainen H, Xue T L, Piironen V. Selenium as an ant- i oxidantand pro -oxidant inryegrass [J]. Plant and Soil, 2000, 225:193-200
    [2]
    Chen T F, Cui X F, Yang F, et al. Stepwise addition of selenium forthe production of high selenium -enriched Spirulina platensis and theeffects on the photosynthetic pigments and proteins [J]. Food and Fer-mentation Industries,2005,31(8): 48-51[陈填烽, 崔小峰, 杨芳,等. 分次加硒法培养高富硒量螺旋藻及其对藻体光合色素和蛋白质含量的影响. 食品与发酵工业, 2005, 31(8): 48-51]
    [3]
    Lin K F, Xu X Q, Jin X, et al. Eco -toxicological effects of seleniumstress on wheat (Triticum aestium L.-and its critical value [J].Journal of Agro-Environment Science, 2004, 23(6): 1082-1085[林匡飞, 徐小清, 金霞, 等. Se 对小麦的生态毒理效应及临界指标研究. 农业环境科学学报,2004, 23(6): 1082-1085]
    [4]
    Lin K F, Xu X Q, Jin X, et al. Eco -toxicological effects of seleniumand its critical value on Oryzasativa [J]. Chinese Journal of AppliedEcology, 2005,16(4): 678-682[林匡飞, 徐小清, 金霞, 等. 硒对水稻的生态毒理效应及临界指标研究. 应用生态学报,2005, 16(4):678-682]
    [5]
    Zheng W J, Yu J, Yang F, et al.Se stress and Se bio- organizationin two species of Spirulina at different growing phases [J]. EcologicScience, 2003,22(4): 305-309[郑文杰, 余景, 杨芳, 等. 两种螺旋藻在不同生长阶段的硒胁迫和生物有机化效应. 生态科学, 2003, 22(4): 305-309]
    [6]
    Rong Z X, Liu H Z, Bao J Q, et al.Microanalysis of the activity ofglutathione peroxidase in whole blood of rat [J]. Prog. Biochem.Biophys, 1994, 21(4): 362-366[荣征星, 刘慧中, 鲍景奇, 等.小鼠全血中谷胱甘肽过氧化物酶活力的微量测定法. 生物化学与生物物理进展, 1994, 21(4): 362-366]
    [7]
    Beauchamp C, Fridovich I. Superoxide disumtase: improved assaysand an assay applicable to acrylamide gel [J]. Annal. Biochem,1971, 44:276-278
    [8]
    Bewley T D. Physiological aspects of desiccation tolerance [J]. An -nal Rev Plant Physiol, 1979,30:195-238
    [9]
    Chance B, Maehly A C. Assay of catalase and peroxidase [J]. Meth -ods in Enzymology,1955, 2: 764-775
    [10]
    Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbatespecific peroxidase in spinach chloroplasts [J]. Plant Cell Physiol,1981, 22:867-880
    [11]
    Boqinoc X H. Plant Biochemical Analytical Method [M]. Beijing:Science Press. 1981, 197-201[波钦诺克 X H. 植物生物化学分析方法. 北京: 科学出版社. 1981, 197-201]
    [12]
    Lin Z F, Li S S, Lin G Z, et al. Superoxide dismutase activity andlipid peroxidation in relation to senescence of rice leaves [J]. ActaBotanicaSinica, 1984, 26(6): 605-615[林植芳, 李双顺, 林桂珠, 等. 水稻叶片的衰老与超氧物歧化酶活性及脂质过氧化作用的关系. 植物学报, 1984,26(6): 605-615]
    [13]
    Zheng W J, He H Z, Huang Z, et al. Effects of the stress of selen- ium or tellurium onthe growth of Spirulina platensis and S. maximum[J]. Marin Sciences, 2003, 27(10): 73-78[郑文杰, 贺鸿志, 黄峙, 等. 硒碲胁迫对两种螺旋藻生长的影响. 海洋科学, 2003,27(10): 73-78]
    [14]
    Hou S F, Xue T L, Tan J A. Glutathione peroxidase and its functionin higher plant [J]. Science Bulletin, 1994, 39(6): 553-556[侯少范, 薛泰麟, 谭见安. 高等植物中的谷胱甘肽过氧化物酶及其功能. 科学通报, 1994, 39(6): 553-556]
    [15]
    Tang X X, Li Y Q, Dong B X. Biological study of toxicity oforganophosphorus pesticides of microalgae.?. Primitive study ofperoxidase inhibitors produced by monocrotophos stress in Dicrateriasp. [J]. MarinScience Bulletin, 1996, 15(1):27-32[唐学玺, 李永祺, 董宝贤. 有机磷农药对海洋微藻致毒性的生物学研究.?. 久效磷诱导叉鞭金藻产生过氧化物酶抑制因子的初步研究. 海洋通报, 1996, 15(1):27-32]
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