RESTRICTION MAP OF MITOCHONDRIAL DNA FROM MEGALOBRAMA AMBLYCEPHALA LIVER AND PRELIMINARY LOCALIZATION OF 12S rRNA GENE OF THE mtDNA

Wuchang Fish (M. amblycephala) liver mtDNA was cleaved by the restriction endonuclease. Upon complete digestion numbers of restriction fragments of BamHI, BglⅡ, BglⅠ, EcorⅠ, HindⅢ and HpaⅡ were determined to be 2,2,3,3,3 and 7 respectively. Molecular weight of the mtDNA was found to be 10×106 dalton or 16.6 kb. A restriction Map of five restriction endonucleases was constructed. 12S rRNA gene of the mtDNA was preliminarily localized by molecular hybridization using 15S rRNA gene from yeast mtDNA as the probe.