DNA DAMAGE AND ALTERATION OF EXPRESSION IN APOPTOSIS RELATED PROTEINS INDUCED BY OKADAICACID IN FL CELLS

The objective of this work was to evaluate deoxyribonucleic acid (DNA) damage and expression of Bcl-2, Bax, p53 proteins in okadaic acid treated human amniotic FL cells1 Cells were cultured for 4 h with 0, 20, 40, 60, 80 and 100 nmol okadaic acid (OA), or 100 nmol OA for periods of 2, 4 and 8 h1 Comet assay results indicated that DNA damage was a dose-dependent increase and western blot analysis showed that the expression of Bcl 2, Bax, p53 proteins was time and dose dependent decrease1 Therefore, it suggested that DNA damage and Bcl-2 might play a partial role in OA-induced FL cell apoptosis, and on the contrary, Bax and p53 were related to the proliferation of FL cell1