Volume 31 Issue 5
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
XING Ming-Luan, LOU Jian-Lin, XU Li-Hong. DNA DAMAGE AND ALTERATION OF EXPRESSION IN APOPTOSIS RELATED PROTEINS INDUCED BY OKADAICACID IN FL CELLS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(5): 661-665.
Citation: XING Ming-Luan, LOU Jian-Lin, XU Li-Hong. DNA DAMAGE AND ALTERATION OF EXPRESSION IN APOPTOSIS RELATED PROTEINS INDUCED BY OKADAICACID IN FL CELLS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(5): 661-665.
shu

DNA DAMAGE AND ALTERATION OF EXPRESSION IN APOPTOSIS RELATED PROTEINS INDUCED BY OKADAICACID IN FL CELLS

  • Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, Hangzhou 310058;
    2. Institute of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou 310058

  • Received Date: January 19, 2006
  • Rev Recd Date: September 18, 2006
  • Published Date: September 24, 2007
    Graphical Abstract
    • Abstract
  • The objective of this work was to evaluate deoxyribonucleic acid (DNA) damage and expression of Bcl-2, Bax, p53 proteins in okadaic acid treated human amniotic FL cells1 Cells were cultured for 4 h with 0, 20, 40, 60, 80 and 100 nmol okadaic acid (OA), or 100 nmol OA for periods of 2, 4 and 8 h1 Comet assay results indicated that DNA damage was a dose-dependent increase and western blot analysis showed that the expression of Bcl 2, Bax, p53 proteins was time and dose dependent decrease1 Therefore, it suggested that DNA damage and Bcl-2 might play a partial role in OA-induced FL cell apoptosis, and on the contrary, Bax and p53 were related to the proliferation of FL cell1
    • FullText(HTML)
    • References (12)
  • [1]
    Fern àndez J J, Candenas M L, Souto M L, et al. Okadaic acid,useful tool for studying cellular processes [J]. Curr Med Chem,2002,9: 229-262
    [2]
    Lago J, Santaclara F, Vieites J M, et al. Collapse of mitochondrial membrane potential and caspases activation are early events in okadaic acidtreated Cacocell [J]. Toxicon, 2005, 46(5): 579-586
    [3]
    Fujiki H, Sueoka E, Komori A, et al. Tumor promotion and TNFagene expression by the okadaic acid class tumor promoters [J]. Environ Carcinog Ecotoxicol Rev, 1997, 15: 1-40
    [4]
    Matias W G, Creppy E E. Transplacental passage of [3H]-okadaic acidin pregnant mice measured by radioactivity and high-performance liquid chromat ography [J]. HumExp Toxicol, 1996, 15(3): 226-230
    [5]
    Singh N P, McCoy M T, Tice R R, et al. A simple technique forquantitation of low levels of DNA damage in individual cells [J].Exp Cell Res, 1988, 175(1): 184-191
    [6]
    Moncada A, Cendan C M, Baeyens J M, et al. Effects of serine/threonine protein phosphatase inhibitors on morphine induced antinociception in the tail flick test in mice [J]. Eur J Pharmacol,2003, 465: 53-60
    [7]
    Le Hegarat L, Puech L, Fessard V, et al. Aneugenic potential ofokadaic acid revealed by the micronucleus assay combined with theFISH technique in CHOK1 cells [J]. Mutagenesis, 2003, 18(3):293-298
    [8]
    Xing M L, Chen J P, Wang X F, et al. Effect of okadaic acid onapoptosis in human amnion cells [J]. Acta Hydrobiologica Sinica,2007, 31(4): 606-608 [邢鸣鸾, 陈加平, 王晓峰, 等. 大田软海绵酸对 FL 细胞凋亡的影响. 水生生物学报, 2007, 31(4):606-608]
    [9]
    Traore A, Baudrimont I.Ambaliou S, et al. DNA breaks and cell cycle arrest induced by okadaic acid in Cacocells, a human colonicepithelial cell line [J]. Arch Toxico, 2001,75(2): 110-117
    [10]
    Bandyopadhyay S, Sengupta T K, Fernandes D J, et al. Taxol andokadaic acidinduced destabilization of bcl 2mRNA is associated withdecreased binding of proteins to a bcl 2 instability element [J].Biochem Pharmacol, 2003,66(7): 1151-1162
    [11]
    Milczarek G J, Chen W, Gupta A, et al. Okadaic acid mediates p53hyperphosphorylation and growth arrest in cells with wild type p53 butincreases aberrant mitoses in cells with nonfunctional p53 [J]. Carcinogenesis, 1999, 20(6): 1043-1048
    [12]
    Yoon S Y, Choi J E, Yoon J, et al. Okadaic acid induces JNK activation, bim overexpression and mitochondrial dysfunction in culturedrat cortical neurons [J]. Neurosci Lett, 2006, 394(3): 190-195
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return