TUDIES ON THE DIFFERENCES OF GENE EXPRESSION IN SCOPHTHALMUS MAXIMUS L.MUSCLE OF LARGE VERSUS SMALL SCALED INDIVIDUALS

In order to understand the molecular basis of fish growth and development, the technique of mRNA differential display was used to analyze the differences in gene expression of muscle tissue in Scophthalmus maximus L muscle of large versus small scaled individuals.Only bands that can be repeated in polyacrylamide gel electrophoresis of duplicate PCR products were used for statistic analysis.Our results showed that the differential expressed gene between the 2 fish flocks was very obvious.18 kinds of primer subsets amplified 527 clear bands of cDNA fragment.In them, the number of differential expressed cDNA fragment is 16 (3.04 %);both fish flocks coincident expression 506 (96.02 %)bands.The differentially expressed cDNA fragments must be related to the growth and development of fish.The screen of these differentially expressed genes will help us to understand the molecular mechanism in Scophthalmus maximus L.growth and development.