STUDY ON THE HUGUANG MICROBIAL ADDITIVE Ⅱ.ESSENTIAL TECHNICAL PARAMETERS IN BATCH CULTURE OF PHOTOSYNTHETIC BACTERIA

Huguang microbial additive is comprised of strains of photosynthetic bacteria,including Rhodopseudomonas and Rhodospirillum. The essential technical parametersin batch culture,such as media,illumination,temperature,pH,aeration and inocula-tion ratio etc. were studied.In general,after incubation for 3-5 days under ap-propriate conditions,the culture became deep red in colur and the cell number inc-reased to about 109/ml.When preserved at room temperature for 6 months, themicrobial additive remained still effective:and when incubated at 40℃ and in darkcondition for 15 days most cells survive in culture.A simple method for quality assay of the microbial additive is presented.