Volume 34 Issue 1
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XU Xiao-Yan, QIAN Hai-Feng, CHEN Wei, JIANG Hong, FU Zheng-Wei. ESTABLISHMENT OF REAL-TIME PCR FOR ANALYZING mRNA ABUNDANCE IN CHLORELLA VULGARIS EXPOSED TO XENOBIOTICS[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(1): 139-143.
Citation: XU Xiao-Yan, QIAN Hai-Feng, CHEN Wei, JIANG Hong, FU Zheng-Wei. ESTABLISHMENT OF REAL-TIME PCR FOR ANALYZING mRNA ABUNDANCE IN CHLORELLA VULGARIS EXPOSED TO XENOBIOTICS[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(1): 139-143.
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ESTABLISHMENT OF REAL-TIME PCR FOR ANALYZING mRNA ABUNDANCE IN CHLORELLA VULGARIS EXPOSED TO XENOBIOTICS

  • College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032 China;
    2. Key Lab of Environment Remediation and Ecological Health, College of Environmental and Resource, Zhejiang University, Hangzhou 310029 China

  • Received Date: March 13, 2008
  • Rev Recd Date: December 25, 2008
  • Published Date: January 24, 2010
    Graphical Abstract
    • Abstract
  • Among the aquatic organisms,algae are one of the most susceptible organisms to pollution in water.Transcriptional abundance has been gradually used as a surrogate for gene expression in environmental biology.In the present study,real-time PCR assay was established to quantify the transcript abundances of three photosystem genes(psaB,psbC and rbcL) in Chlorella vulgaris,and the acute toxicity of three herbicides(glufosinate,atrazine and diclofop-methyl) was assessed using this method.The results demonstrated that real-time PCR is an efficient technique to assess the toxicity of xenobiotic compounds in aquatic system.
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