LIANG Yong, XU Ying, YANG Fang-xing, WANG Jian-wei. INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP(CYPRINUS CARPIO)AND WUCHANGFISH(MEGALOBRAMA AMBLYCEPHALA YIH)[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(4): 317-321.
Citation: LIANG Yong, XU Ying, YANG Fang-xing, WANG Jian-wei. INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP(CYPRINUS CARPIO)AND WUCHANGFISH(MEGALOBRAMA AMBLYCEPHALA YIH)[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(4): 317-321.

INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP(CYPRINUS CARPIO)AND WUCHANGFISH(MEGALOBRAMA AMBLYCEPHALA YIH)

  • Received Date: August 21, 2001
  • Rev Recd Date: September 03, 2001
  • Published Date: July 24, 2002
  • Vitellogenin was induced in immature common carp(Cyprinus carpio L.)and immature Wuchangfish(Megalobrama amblycephala Yih)by intraperitoneal injection of xenobiotic estrogen 17α-ethinyloestradiol. By using the anion-exchange chromatography on a DEAE-Sephrarose CL-6B column, the vitellogenin in plasma from two cyprinid fish species was effectively purified. The results of SDS-PAGE show that the molecular weights of vitellogenin monomer in two cyprinid fish species are around 170KD and 150KD respectively.
  • [1]
    Copeland P A et al. Vitellogenin levels in male and female rainbow trout, Salmo gairdneri Richardson, at various stages of the reproductive cycle [J].Comp. Biochem. Physiol. B. 1986, 83: 487-493[2] SoléM et al. vitellogenin induction and other biochemical responses in carp, Cyprinus carpio, after experimental injection with 17α-ehynylestradiol[J]. Arch. Environ. Contam. Toxicol. 2000, 38: 494-500[3] Purdom C E et al. Estrogenic effects of effluents from sewage treatment works[J].Chem. Ecol. 1994, 8: 275-285[4] Jean M W et al. In vitro vitellogenin production by carp(Cyprinus carpio)hepatocytes as a screening method for determining(Anti)estrogenic activity of xenobiotics[J]. Toxicology and Applied Pharmacology. 1999, 157: 68-76[5] Endocrine Disrupter Screening and Testing Advisory Committee(EDSTAC)finnal report [R]. 1998, EPA/742/R-98/003[6] Heppell S A et al. "Universal" assay of vitellogenin as a biomarker for envrionmental estrogens [J]. Environ. Health Perspect. 1995, 103: 9-15[7] Charles R et al. An in vivo testing system for endocrine disruptors in fish early life stages using induction of vetellogenin [J]. Environ Toxicol Chem. 1999 18: 227-347[8] Janssen P A H et al. Environmental pollution caused elevated concentrations of oestradiol and vitellogenin in the female flounder, Platichthys flesus(L.)[J] .Aquatic Toxicology. 1997 39: 195-214[9] Oliana Carnevali et al. Comparative studies of fish, amphibian, and reptilian vitellogenins [J].J. Experimental Zoology. 1991, 259: 18-25[10] Louise G P et al. Fathead minnow(Pimephales promelas)vitellogenin: purification, characterization and quantitative immunoassay for the detection of estrogenic compounds [J]. Comp Biochem Physiol. 1999, 123C: 113-125[11] Christer Silversand et al. Isolation, immunochemical detection and observations of the instability of vitellogenin from four teleosts [J]. J. Experimental Zoology. 1993, 267: 587-597[12] Fancois B et al. Two-step purification method of vitellogenin from three teleost fish species: rainbow trout(Oncorhynchus mykiss), gudgeon(Gobio gobio)and chub(Leuciscus cephalus)[J].Journal of Chromatography B.2000, 737: 3-12[13] Bradford M M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding [J].Anal. Biochem. 1976, 72: 248-254[14] Laemmli, U.K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4 [J]. Nature. 1970, 227: 680-685
  • Cited by

    Periodical cited type(8)

    1. 王大伟,邢盈,蒋成宇,胡淼,赵金良. 放养密度对鳜幼鱼HPI轴激素、应激酶活力及呼吸频率的影响. 淡水渔业. 2025(02): 26-32 .
    2. 李小红,付成,付世建. 焦虑对雌性成年斑马鱼热耐受和游泳能力的影响. 水生生物学报. 2024(09): 1566-1572 . 本站查看
    3. 龙珍满,朱峰跃,郭杰,俞立雄,郑永华,段辛斌. 捕食胁迫对“四大家鱼”幼鱼生理反应的影响. 渔业科学进展. 2023(03): 111-123 .
    4. 付翔,付成,樊捷,肖玲韬,付世建. 个性对中华倒刺鲃群体运动特征和食物占有能力的影响. 水生生物学报. 2022(06): 838-846 . 本站查看
    5. 吴雪阳,郭红会,况宇,张策,杨慧,汤蓉,张曦,李大鹏,李莉. 氨氮胁迫下不同食性鱼类仔鱼抗氧化和非特异性免疫的差异响应. 水生生物学报. 2022(08): 1237-1248 . 本站查看
    6. 周龙艳,李秀明,付世建. 中华倒刺鲃和胭脂鱼游泳行为、应激和免疫能力对短期禁食的响应. 生态学报. 2022(17): 7288-7295 .
    7. 蔡露,Katopodis Christos,金瑶,黄应平,韩德举,胡望斌,陈小娟,陶江平,侯轶群. 中国鲤科鱼类游泳能力综合分析和应用. 湖泊科学. 2022(06): 1788-1801 .
    8. 王亚,付成,胡月,付世建. 四种鲤科鱼类对捕食胁迫行为响应的种间比较. 水生生物学报. 2021(05): 1154-1163 . 本站查看

    Other cited types(2)

Catalog

    Article views PDF downloads Cited by(10)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return