LIANG Yong, XU Ying, YANG Fang-xing, WANG Jian-wei. INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP(CYPRINUS CARPIO)AND WUCHANGFISH(MEGALOBRAMA AMBLYCEPHALA YIH)[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(4): 317-321.
Citation: LIANG Yong, XU Ying, YANG Fang-xing, WANG Jian-wei. INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP(CYPRINUS CARPIO)AND WUCHANGFISH(MEGALOBRAMA AMBLYCEPHALA YIH)[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(4): 317-321.
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INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP(CYPRINUS CARPIO)AND WUCHANGFISH(MEGALOBRAMA AMBLYCEPHALA YIH)

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  • Vitellogenin was induced in immature common carp(Cyprinus carpio L.)and immature Wuchangfish(Megalobrama amblycephala Yih)by intraperitoneal injection of xenobiotic estrogen 17α-ethinyloestradiol. By using the anion-exchange chromatography on a DEAE-Sephrarose CL-6B column, the vitellogenin in plasma from two cyprinid fish species was effectively purified. The results of SDS-PAGE show that the molecular weights of vitellogenin monomer in two cyprinid fish species are around 170KD and 150KD respectively.
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