HUANG Xiao-Hong, CHEN Hong-Hui, LIU Jian-Chang, JIN Yan-Dong, XIAO Qi-Tao. EFFECTS OF SOME HEAVY METAL ON THE N-ACETYL-β-D-GLUCOSAMINIDASE FROM ERIOCHEIR SINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(6): 850-855.
Citation: HUANG Xiao-Hong, CHEN Hong-Hui, LIU Jian-Chang, JIN Yan-Dong, XIAO Qi-Tao. EFFECTS OF SOME HEAVY METAL ON THE N-ACETYL-β-D-GLUCOSAMINIDASE FROM ERIOCHEIR SINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(6): 850-855.

EFFECTS OF SOME HEAVY METAL ON THE N-ACETYL-β-D-GLUCOSAMINIDASE FROM ERIOCHEIR SINENSIS

  • Received Date: August 23, 2007
  • Rev Recd Date: May 11, 2008
  • Published Date: November 24, 2008
  • The effects of heavy metal ions(Cu2+,Pb2+,Zn2+ and Ag+)on N-Acetyl-β-D-glucosaminidase from Eriocheir sinensis were studied.The results showed that Cu2+,Pb2+ and Zn2+ inhibited the activity of enzyme in vary degree.Cu2+ and Zn2+ were both reversible non-competitive inhibitors.The inhibition constants of free enzyme(KI) of Cu2+ and Zn2+ were 1.25 mmol/L and 8.10mmol/L,respectively.Pb2+ was reversible mixed-type inhibition.The inhibition constants of free enzyme(KI) and enzyme-substrate complex(KIS) of Pb2+ were determined to be 10.44mmol/L and 2.18mmol/L,respectively.Ag+ activated the enzyme at low concentrations,but it inhibited the enzyme at higher concentrations as a reversible un-competitive inhibitor.The inhibition constants(KIS) was 204.51mmol/L.
  • [1]
    X iY Y, Wang L, Y ang X Q, et a l. Th e accumulation ofH g2+ inmajor t issues and organ s ofE rioch eir sin en sis [J]. Acta Zoolog icaSin ica, 2001, 47( sup): 92-95 [席玉英, 王兰, 杨秀清, 等. 汞在中华绒螯蟹主要组织器官中的积累. 动物学报, 2001, 47( sup ): 92-95]
    [2]
    Yu F J. Study tox ic effects of two heavymetals of Pb and Cd onE riocheir sinensis [D]. Shangha:i E as t Ch ina Normal Un iversity.2005 [于丰军. 铅和镉两种重金属对中华绒螯蟹的毒性效应研究. 上海: 华东师范大学. 2005]
    [3]
    Xu Z K, H e Q Y. Th e emphases, keystones and examp les ofE rio2cheir sinensis nu isance2free breed [M]. Scien tific and T echn icalDocumen ts Pub lish ingH ou se. 2005 [徐在宽, 何群益. 中华绒螯蟹无公害养殖重点、难点与实例. 科学技术文献出版社.2005]
    [4]
    Funke B, Sp ind ler K D. Characterization of ch itin ase from BrineShrimpA rtem ia [J] 1Comp B ioch em Phys io,l 1989, 94B ( 4 ):691-695
    [5]
    HuangX H, Chen H H, Hu angY F. Prelim inary stud ies on isola2t ion, pu rification and some p roperties of the B2N2A cety l2D2glu2cosam in idase from E riocheir sin en sis [J]. Acta H yd robiologicaSinica, 2007, 31 ( 4): 563-569 [黄小红, 陈宏惠, 黄一帆. 中华绒螯蟹( E rioch eir sinensis) N2乙酰2B2D2氨基葡萄糖苷酶的分离纯化及性质的初步研究. 水生生物学报, 2007, 31 ( 4 ):563-569]
    [6]
    H uangX H, Ch enQ X, YouM S, et al. Isolat ion, pu rification andpartial characterization of the B2N2Acetyl2D2glu cosam in idase fromth e pupae ofH elicoverpa a rmig era [J], Acta E ntomolog ica Sini2ca, 2005, 48( 4 ): 498-502 [黄小红, 陈清西, 尤民生, 等. 棉铃虫N2乙酰2B2D2氨基葡萄糖苷酶的分离纯化及性质的初步研究. 昆虫学报, 2005, 48( 4 ): 498-502]
    [7]
    WangL, YangX Q, WangQ, et a l. The accumu lation of Cd2+ andthe effect on est in five t issues and organ s of E rioch eir sin en sis [J]. Acta Z oologica Sin ica, 2001, 47 ( sup): 96-100 [王兰, 杨秀清, 王茜, 等. 镉在中华绒螯蟹五种组织器官的积累及对酯酶同工酶的影响. 动物学报, 2001, 47 ( sup): 96-100]

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