NIE Liu-wang, SHAN Xiang-nian, WANG Ming, GUO Chao-wen, LU Xiao-xuan. THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOUR Sox GENES IN THE TRIONYX SINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 245-250.
Citation: NIE Liu-wang, SHAN Xiang-nian, WANG Ming, GUO Chao-wen, LU Xiao-xuan. THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOUR Sox GENES IN THE TRIONYX SINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 245-250.

THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOUR Sox GENES IN THE TRIONYX SINENSIS

  • Received Date: April 06, 2000
  • Rev Recd Date: December 19, 2000
  • Published Date: May 24, 2001
  • The Sox genes of Trionyx sinensis were amplified and cloned using PCR. The four different fragments of Sox gene HMG-box DNA were selected and sequenced. The results indicated that the Sox gene is conservative in phylogeny, in which the amino acid sequence encode by TSSox4(The Sox of T. Sinensis)is identical to those encode by LF4(bird)or differ from SOX4(human)and Sox4(mouse)only on one amino acid; The amino acid sequences encode by TSSox5 have an amino acid difference from those encode by LF5(bird); TSSox1 has about 75% identical to SRY HMG-box at the nucleotide level, and is most resemblance among four TSSox. Phylogenetic analysis of available 32 Sox sequences including SRY/Sry sequence suggests that there wolud be a high degree of divergence between any possible immediate common ancestor of the T. Sinensis Sox sequence and the SRY(Sry)sequences.
  • [1]
    Janzen J F.Environmental sex determination in reptiles: Ecology,Evolution,and Experimental design[J].The Quarterly Review of Biology,1991,66(2):149—179[2] Spotile L D,Kaufer N F,Theriot E.Sequence analysis of the ZFY and sox genes in the turtle,chelydra serpentina[J].Molecular Phylogenetic and Evolution,1994,3(1):1—9[3] Western P S,Harry J L,Graves J.a,et al.Temperature-dependent sex determination: upregulation of SOX9 expression after commitment to male development[J].Development-Dynamics,1999,214(3):171—177[4] 聂刘旺,郭超文,汪鸣.中华鳖性别决定机制的研究[C],安徽省第三届“兴皖之光”青年学术年会论文集合肥;安徽科技出版社,1998,59—64[5] 聂刘旺,单祥年,郭超文.两种龟类Sox基因的PCR扩增及SSCP分析的研究[J].应用与环境生物学报 1999,5(4);378—381[6] Sambrook J,Frisch E F,Maniatis T.Molecular cloning—a laboratory manual(2 nd ed)[M] Cold Spring Harbor Laboratory Press.1989,[7] Capel B.Sex in the 90s: SRY and the switch to male pathway[J].Annu.Rev.Physial.1998,60:497—521[8] Elreavey K.Vilain E.Kaufer NF.et al: A regular cascade hypothesis for mammalian sex determination: SRY represses a negative regulator of male development[J].PNAS of USA,1993,90:3368[9] Vilain E,McCobe E.R Mammalian sex determination: From gonads to brain[J].Mol.Genet.Metab.1998,65(2):74—84[10] Schilham MW,Moerer P,Cumano A.HC Clevers.,Sox4 facilitates thymocyte differentiation[J].European Journal of Immunology,1997,27(5):1292—1295[11] Ahn S G.,Cho G H.,Jeong S Y.et al.Identification of cDNAs for Sox4,an HMG-Box\protein,and a novel human homology of yeast splicing factor SSF-1 differentially regulated during apoptosis induced by prostaglandin A2/deltal2-PGJ2 in Hep3B cells[J] Biochemical And Biophysical Research Communications,1999,260(1):216—221

    Janzen J F.Environmental sex determination in reptiles: Ecology,Evolution,and Experimental design[J].The Quarterly Review of Biology,1991,66(2):149—179[2] Spotile L D,Kaufer N F,Theriot E.Sequence analysis of the ZFY and sox genes in the turtle,chelydra serpentina[J].Molecular Phylogenetic and Evolution,1994,3(1):1—9[3] Western P S,Harry J L,Graves J.a,et al.Temperature-dependent sex determination: upregulation of SOX9 expression after commitment to male development[J].Development-Dynamics,1999,214(3):171—177[4] 聂刘旺,郭超文,汪鸣.中华鳖性别决定机制的研究[C],安徽省第三届“兴皖之光”青年学术年会论文集合肥;安徽科技出版社,1998,59—64[5] 聂刘旺,单祥年,郭超文.两种龟类Sox基因的PCR扩增及SSCP分析的研究[J].应用与环境生物学报 1999,5(4);378—381[6] Sambrook J,Frisch E F,Maniatis T.Molecular cloning—a laboratory manual(2 nd ed)[M] Cold Spring Harbor Laboratory Press.1989,[7] Capel B.Sex in the 90s: SRY and the switch to male pathway[J].Annu.Rev.Physial.1998,60:497—521[8] Elreavey K.Vilain E.Kaufer NF.et al: A regular cascade hypothesis for mammalian sex determination: SRY represses a negative regulator of male development[J].PNAS of USA,1993,90:3368[9] Vilain E,McCobe E.R Mammalian sex determination: From gonads to brain[J].Mol.Genet.Metab.1998,65(2):74—84[10] Schilham MW,Moerer P,Cumano A.HC Clevers.,Sox4 facilitates thymocyte differentiation[J].European Journal of Immunology,1997,27(5):1292—1295[11] Ahn S G.,Cho G H.,Jeong S Y.et al.Identification of cDNAs for Sox4,an HMG-Box\protein,and a novel human homology of yeast splicing factor SSF-1 differentially regulated during apoptosis induced by prostaglandin A2/deltal2-PGJ2 in Hep3B cells[J] Biochemical And Biophysical Research Communications,1999,260(1):216—221

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