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LIU Li-Na, WANG Qi-Hua, ZHANG En-Dong, ZHANG Li-Jia. CRYOPRESERVATION OF ISOCHRY SIS ZHANJ IANGENSIS BY ENCAPSULATION 2VITRIFICATION[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 169-173.
Citation: LIU Li-Na, WANG Qi-Hua, ZHANG En-Dong, ZHANG Li-Jia. CRYOPRESERVATION OF ISOCHRY SIS ZHANJ IANGENSIS BY ENCAPSULATION 2VITRIFICATION[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 169-173.
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CRYOPRESERVATION OF ISOCHRY SIS ZHANJ IANGENSIS BY ENCAPSULATION 2VITRIFICATION

  • College of Life Sciences, Liaoning Normal University, Liaoning 116029

  • Received Date: August 21, 2006
  • Rev Recd Date: December 16, 2006
  • Published Date: March 24, 2007
    Graphical Abstract
    • Abstract
  • Isochrysis zhanjiangensis was preserved in liquid nitrogen by encapsulation-vitrification. The followingfactors affect the viability of the alga such as the composition and the concentration of loading solution and loading time, the time of dehydration, the concentration of washing solution and washing time were studied. The result showed that the high viability of 54 % was obtained when the beads containing the algal cells were loaded with 50 % PVS(PVS:30 %(W/V) glycerol, 20 %(W/V) ethylene glycol and 10 %(W/V) dimethyl sulfoxide inf/2 medium)for 4.5h at 20 ℃, then dehydrated with 100 % PVSfor 50 min at 0 ℃ prior to a direct plunge into liquid nitrogen and washed by 1mol/L sorbitol for 40min after warming in 40 ℃water bath. The method of encapsulation2vitrification do not need special equipments, simplifies the operation procedure of cryopreservation, so it is a potential method for preservation of I. zhanjiangensis and other algae.
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    • References (27)
  • [1]
    DayJ G. Cryo conservationof microalgae and cyanobacteria [J]. Cry-Letters (Supplement), 1998, 1: 7—14
    [1]
    [梁宏,王起华. 植物种质的玻璃化超低温保存. 细胞生物学杂志,2005,27:43—45]
    [2]
    Taylor R, Fletcher R. Cryopreservation of eukaryotic algaa review ofmethodologies [J]. Journal of Applied Phycology, 1999, 10: 481—501
    [2]
    [J]. Plant Cell Reports, 1999, 19: 150—155
    [3]
    Wang Q H, Zhang ED, Zhou C Y.A surveyof studieson the cyrop-reservation of algae germplasm [J]. Chinese Bulletin of Botany,2002, 19(1): 21—29 [王起华,张恩栋,周春影. 藻类种质超低温保存研究概况. 植物学通报,2002,19(1):21—29]
    [4]
    Hirata K, Phunchindawan M, Tukamoto J, et al. Cryopreservation ofmicroalgae using encapsulation dehydration [J]. Cry Letters, 1996,17: 321—328
    [5]
    Xiang L X, Shao J Z. Cryopreservation of Chlamydomonas renhardtiiby vitrification [J]. Chinese Journal of Cell Biology, 2001, 23(2):110—113 [项黎新,邵健忠. 衣藻细胞玻璃化法超低温保存技术的研究. 细胞生物学杂志,2001,23(2):110—113]
    [6]
    Cai X N, Chen S F, ChenJ, Liu S H. Cryopreservation of Chlorellavulgaris by vitrification [J]. Plant Physiology Communications,2004, 4(5):599—601 [蔡小宁,陈舒泛,陈俊,刘少华. 小球藻的玻璃化超低温保存法. 植物生理学通讯,2004,4(5):599—601]
    [7]
    Wang Q H, Li H, Zhang ED, Gao YP, Li D P. Cryopreservationofmarine golden2brown algae (Chrysophyceae) used as aquacultural dietby encapsulation2dehydration [J]. Oceanologia et Limnologia Sinica,2005, 36(2):172—178 [王起华,李贺,张恩栋,高艳萍,李大朋. 用包埋脱水法冰冻保存海洋饵料金藻. 海洋与湖沼,2005,36(2):172—178]
    [8]
    Matsumoto T, Sakai A, T akahashi C, Y amada K.Cryopreservation of invitro grown apical meristemsof wasabi (wasabia japonica) by encapsulation vitrification method [J]. Ctyo-lettters, 1995, 16: 189—196
    [9]
    Wu X M, Tang H R. Research advances in cryopreservation of plantgermplasm by encapsulation vitrification method [J]. Chinese Bulletinof Botany, 2005, 22(2):238—245 [吴雪梅,汤浩茹. 包埋玻璃化法超低温保存植物种质的研究进展. 植物学通报,2005,22(2):238—245]
    [10]
    Liang H, Wang Q H. The cryopreservation of plant germplasm by vit2rification [J]. Chinese Journal of Cell Biology, 2005, 27:43—45
    [11]
    Liang Y, Sun S C, Wei J G. Culture techniques of organisms used asfood in mariculture [M].Qingdao: Qingdao Ocean University Press.1998, 26—27 [梁英,孙世春,魏建功. 海水生物饵料培养技术. 青岛:青岛海洋大学出版社. 1998,26—27]
    [12]
    Wang Q H, Shi R F, Cheng A H. Cryopreservationof 3 golden2brownalgae(Chrysophyta) used asfood in mariculture [J]. Journal of Fishery Sciences of China, 1999, 6(2):89—92 [王起华,石若夫,程爱华. 三种饵料金藻超低温保存技术的研究. 中国水产科学,1999,6(2):89—92]
    [13]
    Mclachlan J. Growth media2marine [A]. In:Stein J R, Handbook ofphycological methods [C]. London: Cambridge University Press.1973, 25—51
    [14]
    Lu J S, Lai L.Biostatistics [M].Beijing: Higher Education Press ofChina.2003, 68—92 [陆健身,赖麟. 生物统计学. 北京:高等教育出版社. 2003,68—92]
    [15]
    Sakai A, K obayashi S, Oiyama I. Crypreservation of nucellar cells ofnavel orange (Citrification Osh. var. brasiliensis Tanaka) by vitrifica2tion [J]. Plant Cell Reports, 1990, 9: 30—33
    [16]
    Guo YX, Liu YJ.Apreliminary studyof suspension cell cryo2preser2vation of rhodiola fastigiata by vitrification [J]. Acta Botanica Borea2li2occidentalia Sinica, 2006, 26(8): 1605—1611 [郭燕霞,刘玉军. 长鞭红景天悬浮培养细胞的玻璃化法超低温保存研究.西北植物学报,2006,26(8): 1605—1611]
    [17]
    Yan Q F, Huang C N. Cryopreservatio of plant tissues and cells byvitrification [J]. Chinese Journal of Cell Biology, 1994, 16 (3):117—122 [严庆丰,黄纯农. 植物组织和细胞的玻璃化冰冻保存. 细胞生物学杂志,1994,16(3):117—122]
    [18]
    Karlsson J O M, T oner M.Longterm storage of tissues by cryopreservation: Critical issues [J]. Biomaterials, 1996, 17(3): 243—256
    [19]
    Liu H Q, Liu W G, G ong Q H, et al. Protoplasts of the algae Por2phyra yezoenisis by vitrification [J].Plant Science, 2004, 166:97—102
    [20]
    Tsukazaki H, Mii M, T okuhara K, Ishikawa K. Cryopreservatio ofDoritaenopsis suspension culture by vitrification [J]. Plant Cell Re2ports, 2000, 19: 1160—1164
    [21]
    Hirai D, Sakai A. Cryopreservatio of in vitro-growm axillary shoot tipmeristems of mint (Mentha spicata L. ) by encapsulation vitrification
    [22]
    Hirai D, Shirai K, Shirai S, Sakai A. Cryopreservation of in vitro-grown meristems of strawberry (Fragaria ⅹananassa Duch. ) by en2capsulation vitrification [J]. Euphytica, 1998, 101: 109—115
    [23]
    Qiao C W, LaamanenJ, Uosukainen M, ValkonenJ P T. Cryopreser vation of in vitro-grown shoot tips of raspberry (Rubus ldaeus L. ) byencapsulation vitrification and encapsulation dehydration [J].PlantCell Reports, 2006, 24: 280—288
    [24]
    Canavate J P, Lubian L M. Some aspects on the cryopreservation ofmicroalgae used as food for marine species [J]. Aquaculture, 1995,136: 277—290
    [25]
    Ma Z Z, Zhang J H. Studies on ultralow temperature maintencespecies technique of microalgae for Mariculture [J]. Journal of Fish2ery Sciences of China, 1997, 4(4): 13—17 [马志珍, 张继红. 海产饵料微藻超低温保存技术的研究. 中国水产科学, 1997, 4(4): 13—17]
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