ZHU Tao, OUYANG Hui, JIN Shi-Wei, XU Ying, SONG Bi-Yu, XU Xu-Dong. ENHANCEMENT OF DIBENZOFURAN DEGRADATION BY JANIBACTER SP. IN AN ACTIVATED SLUDGE REACTOR AND GENETIC ANALYSES OF THE DEGRADING CAPABILITY[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(4): 499-502.
Citation: ZHU Tao, OUYANG Hui, JIN Shi-Wei, XU Ying, SONG Bi-Yu, XU Xu-Dong. ENHANCEMENT OF DIBENZOFURAN DEGRADATION BY JANIBACTER SP. IN AN ACTIVATED SLUDGE REACTOR AND GENETIC ANALYSES OF THE DEGRADING CAPABILITY[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(4): 499-502.

ENHANCEMENT OF DIBENZOFURAN DEGRADATION BY JANIBACTER SP. IN AN ACTIVATED SLUDGE REACTOR AND GENETIC ANALYSES OF THE DEGRADING CAPABILITY

  • Received Date: November 03, 2005
  • Rev Recd Date: November 08, 2006
  • Published Date: July 24, 2007
  • Dibenzofuran (DF) is one of the model compounds for the study of biodegradation of dioxin-like pollutants. This paper presents studies of the enhancing effect of a Janibacter strain on the degradation of DF by activated sludge and analyses of its DF-degrading genes. When 1% or 5 % Janibacter cells were added to the activated sludge reactor, DF of 56 mg/L was almost completely degraded after d8h or 24h, while in the control reactor, 38 % of the DF remained after 48h. A stretch of 4018-bp DNA region containing dfdA1A2A3A4 cluster was cloned with PCR and sequenced and found to be almost identical to that from Janibacter strain YK3, except 5 sbustitutions. To test whether the dfdA cluster is located on a large plasmid, we examined 27 single colonies grown on rich medium plates at 37℃ with PCR and found all tested colonies lost the dfdA cluster at high temperature. This result indicated that the gene cluster was readily cured from the cells, hence it must be located on an extra-chromosomal genetic element. Mutants abolished of the gene cluster were unable to grow in medium with DF as the sole carbon source. The implications of these results to the application of DF-degrading strains in activated sludge reactors are discussed.
  • [1]
    Wang X Y. Microbes degrading dioxin and related toxins[J]. The Bulletin of Microbiology, 2001, 28:101-104[王修垣.降解二 英及其相关毒物的微生物.微生物学通报, 2001, 28: 101-104]
    [2]
    Wittich R. Degradation of dioxin like compounds by microorganisms[J]. Appl Microbiol Biotechnol, 1998, 49: 489-499v[3] Mohn W, Tiedje J. Microbial reductive dehalogenation[J]. Microbiol Rev, 1992, 56: 482-507
    [4]
    Wittich R, Wilkes H, Sinnwell V, et al. Metabolism of dibenzop dioxin by Sphingomonas sp. strain RW1[J]. Appl Environ Microbiol,1992, 58:1005-1010
    [5]
    Ishiguro T, Ohtake Y, Nakayama S, et al. Biodegradation of diben zofuran and dioxins by Pseudomonas aeruginosa and Xanthomonasmaltophilia[J]. Environ Technol, 2000, 21:1309-1316
    [6]
    Iida T, Mukouzaka Y, Nakamura K, et al. Isolation and characteri-zation of dibenzofuran degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofurandegrading Rhodococcus species[J]. Biosci Biotechnol Biochem, 2002, 66:1462-1472
    [7]
    Iida T,Mukouzaka Y,Nakamura K, Kudo T. Plasmid -borne genes code for an angular dioxygenase involved in dibenzofuran degradationby Terrabacter sp. strain YK3[J]. Appl Environ Microbiol, 2002,68: 3716-3723

Catalog

    Article views (958) PDF downloads (494) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return