Volume 33 Issue 3
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ZHANG Ting, LU MaiXin, YE Xing, QUAN YingChun, GAO FengYing, HUANG ZhangHan, BAI Jun-Jie. THE GENETIC STRUCTURE OF FOUR POPULATIONS OF OREOCHROMIS AUREUS BY MICROSATELLITE DNA ANALYSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 498-508.
Citation: ZHANG Ting, LU MaiXin, YE Xing, QUAN YingChun, GAO FengYing, HUANG ZhangHan, BAI Jun-Jie. THE GENETIC STRUCTURE OF FOUR POPULATIONS OF OREOCHROMIS AUREUS BY MICROSATELLITE DNA ANALYSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 498-508.
shu

THE GENETIC STRUCTURE OF FOUR POPULATIONS OF OREOCHROMIS AUREUS BY MICROSATELLITE DNA ANALYSIS

  • Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, China;
    2. Fishery College of Guangdong Ocean University, Zhanjiang 524025, China

  • Received Date: August 14, 2007
  • Rev Recd Date: December 18, 2008
  • Published Date: May 24, 2009
    Graphical Abstract
    • Abstract
  • Genetic diversity and phylogenetic relationships among four breed varieties of Oreochromis aureus (83), O. au-reus (02), O. aureus (05) and red O. aureus were studied using microsatellite analysis technology. 19 pairs of microsatel-lite DNA primers of Oreochromis niloticu registered in GenBank which were selected and proved to amplify successfully on Oreochromis aureus by pre-experiment were used. The PCR reaction condition especially concentration of Mg2+and annealing temperature were adjusted for each pair of primers. Amplified PCR products were electrophoresed in PAGE and stained according to the silver stain procedures and then imagined by Alphalmager. A total of 82 alleles were obtained from the four populations. The number of alleles for each locus ranged from 2-8, and the average alleles was 4.3 each locus, Average observed heterozygosity and average expected heterozygosity were calculated by POPGENE3.2 software. The results showed that the observed value of average heterozy-gosity of the four O. aureus populations ranged from 0.154 to 0.391. The expected value of average heterozygosity ranged from 0.181 to 0.428, and the average polymorphism information contents ranged from 0.1513 to 0.3882, respectively. The lowest genetic diversity level was found in the O. aureus (02). The highest genetic similarity index (0.9422) and the lowest genetic distance (0.0596) were found between O. aureus (05) and O. aureus (83), which indicated a closer phy-logenetic relationship between these two populations. On the other hand, the lowest genetic similarity index (0.6977) and the highest genetic distance (0.3599) existed between O. aureus (83) and red O. aureus, which suggested a farthest phy-logenetic relationship between these two populations. The evaluation results of the genetic deviation index D revealed that there were loci departed from Hardy-Weinberg equilibrium in all the four O. aureus populations. Results of null allele anal-ysis by MicroChecker showed that there were loci showing evidences for null alleles in the populations except red O. aureus population, including one locus for O. aureus (83), two for O. aureus (02) and seven for O. aureus (05). Such results suggested that locus with evidence for null alleles in the populations except red O. aureus might be one of the reasons cau-sing deviation of Hardy-Weinberg equilibrium. Phylogenetie relationship of the four populations was analyzed by using UPGMA methods based on their genetic distances and the results showed that O. aureus (83) and O. aureus (05) popula-tions were clustered in the first group, the O. aureus (02) in the second group, while the red O. aureus was in a separate group.
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    • References (25)
  • [1]
    Xu P,Zhou L H,Tian L P,et al.Isolation of microsatellite markers from Fenneropenaeus chinensis ESTs[J].Journal of Fisheries of China,2003,27(3):213-219[徐鹏,周令华,田丽萍,等.从中国对虾ESTs中筛选微卫星标记的研究.水产学报,2003,27(3):213-219]
    [2]
    Li Z Z,Wu T T,eta].Genetic mark of Oreochromis aureus and Oreochromis niloticus[J].Journal of Gansu Agricultural University,2000,35(1):29-32[李志忠,吴婷婷.奥利亚罗非鱼和尼罗罗非鱼的遗传标记.甘肃农业大学学报,2000,35(1):29-32]
    [3]
    Zhou Y Y,Ye X,Lu M X,et al.AFLP analysis of two tilapia hybridization combinations and their hybrids F《,1》[J].Journal of Agricultural Biotechnology,2007,15 (3):537-538[周远扬,叶星,卢迈新,等.两个罗非鱼杂交组合亲本群体与子一代的AFLP分析.农业生物技术学报,2007,15(3):537-538]
    [4]
    Cao Y,Xia D Q,Wu T T,et al.Studies on the genetic variation in mitochondrial DNA of Oreocheromis niloticus and 0.aureus[J].Journal of Fisheries of China,1997,21 (4):360-365[曹萤,夏德全,吴婷婷,等.尼罗非鲫和奥利亚非鲫线粒体DNA遗传差异的研究.水产学报,1997,21(4):360-365]
    [5]
    Wang T,Li Z,Xia D.Analysis of the gene polymorphism of O.aureus and O.niloticus by using RAPD[J].Developmental & Reproductive Biology,1999,2(19):19-24
    [6]
    Zhang T,Lu M X,Ye X,et al.Selection of microsatellite loci of Oreochromis aureus[J].Journal of Guangdong Ocean University,2007,27(3):6-10[张庭,卢迈新,叶星,等.奥利亚罗非鱼微卫星位点的筛选.广东海洋大学学报,2007,27(3):6-10]
    [7]
    Xu S B,Tao Y F,Yang Z Q.A simple and rapid methods used for silver staining and gel preservation[J]. Hereditas, 2002,24(3):335-336[许绍斌,陶玉芬,杨昭庆.简单快速的DAN银染和胶保存方法.遗传, 2002,24(3):335-336]
    [8]
    Botstein D,White R L,Skolnick M.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].Am J Hum Genet,1980,32:314-331
    [9]
    Nei M,Roychandhury A K.Sampling variances of heterozygosity and genetic distance[J]. Genetics,1974,76(2):379-390
    [10]
    Van O C,Hutchinson W F,Willis D P M,et al.Micro-checker:software for identifying and correcting genetyping errors in microsatellite data[J].Molecular Ecology Notes,2004,4:535-538
    [11]
    Nei M,Maruyama T,Chakraborty R.The bottleneck effect and genetic variability in Populations[J].Evolution,1975,29:1-10
    [12]
    Xiao T Y,Zhang X W,Zhang H Y,et al.RAPD analysis of genetic diversity on genomic DNA among 4 species of pelteobagrus of Dongting Lake[J].China Biotechnology,2004,24 (3):85-89[肖调义,张学文,章怀云,等.洞庭湖四种黄颡鱼基因组DNA遗传多样性的RAPD分析.中国生物工程杂志,2004,24(3):85-89]
    [13]
    Li J L,Mang L,Shi Y F.Evaluation of genetic diversity of Mongolian horse and thoroughbred horse using microsatellite marker[J].Acta Veterinaris et Zootechnica Sinic,2005,36(1):6-9[李金莲,芒来,石有斐.利用微卫星标记对蒙古马和纯血马遗传多样性的研究.畜牧兽医学报,2005,36(1):6-9]
    [14]
    Ma H Y,Jiang Y L,Guo J F,et al.Genetic diversity analysis on Pseudobagrus fulvhtraco in Dongping Lake using microsatellite markers[J].Acts Laser Biology Sinica,2006,15(2):136-139[马洪雨,姜运良,郭金峰,等.利用微卫星标记分析东平湖黄颡鱼的遗传多样性.激光生物学报,2006,15(2):136-139]
    [15]
    Lu S Q,Liu Z,Liu H Y,et al.Microsatellite DNA analysis of genetic diversity and the phylogenetic relationships of four breed varieties of Carassius sp.[J].Journal of Fishery Sciences of China, 2005,12(4):371-376[鲁双庆,刘臻,刘红玉,等.鲫鱼4群体基因组DNA遗传多样性及亲缘关系的微卫星分析.中国水产科学,2005,12(4):371-376]
    [16]
    Liang L Q,Chang Y M,Dong C Z,et al.Genetic analysis for Hu cho taimen in Wusuli River with microsatellites[J].Journal of Fisheries of China,2004,28(3):241-244[梁利群,常玉梅,董崇智,等.微卫星DNA标记对乌苏里江哲罗鱼遗传多样性的分析.水产学报,2004,25(3):241-244]
    [17]
    Du C B,Sun X W,Lou Y D,et al.The genetic heterozygosity analysis to wild carp and two cultivated strains of common carp using microsatellite technique[J].Journal of Shanghai Fisheries University,2000,9(4):285-289[杜长斌,孙孝文,楼允东,等.应用微卫星技术对野鲤和两种鲤选育品系的遗传多样性分析.上海水产大学学报,2000,9(4):285-289]
    [18]
    Liao X L,Yu X M,Tan D Q,et al.Microsatellite DNA analysis of genetic diversity of grass carp in Yangtze River system[J].Acts Hydrobiologica Sinica,2005,29 (2):113-119[廖小林,俞小牧,谭德清.等.长江水系草鱼遗传多样性的微卫星DNA分析.水生生物学报,2005,29(2):113-119]
    [19]
    Li J L,Zhou Z J.Introduction and research of blue tilapia in China mainland[J].Journal of Zhejiang Ocean University (Natural Science),2000,19(3):261-265[李家乐,周志金.中国大陆奥利亚罗非鱼的引进和研究.浙江海洋学院学报(自然科学版),2000,19(3):261-265]
    [20]
    Xu Y D,Zhong J X,Zheng S L,et al.RAPD study of testes genomic DNA from T.aurea and T.nilotica[J].Journal of Xiamen University (Natural Science),1999,38(5):793-796[许玉德,钟建新,郑森林,等.尼罗罗非鱼和奥利亚罗非鱼性腺的RAPD分析.厦门大学学报,1999,38(5):793-796]
    [21]
    Xia D Q,Cao Y,Wu T T,et al.A study on genetic variation of tilipias fish with RAPD analysis and it's application to heterosis[J].Journal of Fisheries of China,1999,23(1):27-32[夏德全,曹萤,吴婷婷,等,用RAPD分析对罗非鱼遗传变异的研究及其对杂种优势的应用.水产学报,1999,23(1):27-32]
    [22]
    Plosky Y,Cahner A,Haberfeld A.DNA Fingerprint bands applied to linkage analysis with quantitative trait loci in chickens[J].Animal Genetics,1993,24:105-110
    [23]
    Baroiller J F,D'Cotta H.Environment and sex determination in farmed fish[J].Comparative Biochemistry and Physiology,2001,130:399-499
    [24]
    Ge X P,Xia D Q,Yu J H.Research progress of fish sex determination[J].Journal of Fishery,Sciences of China,2002,9 (4):371-374[戈贤平,夏德全,俞菊华.鱼类性别决定的研究进展.中国水产科学,2002,9(4):371-374]
    [25]
    Zhu J,Min K H.The general study of China tilapia breeding development[J].Fisheries Science and Technology Industry,2003,1:6-8[朱健,闵宽洪.我国罗非鱼育种研究发展概况.渔业科技产业,2003,1:6-8]
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