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CHEN WenBo, LIU LiLi, LI WenSheng, LIN HaoRan. ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM HEPATOPANCREATIC cDNA LIBRARY OF COMMON CARP(CYPRINUS CARPIO) AND CHARACTERIZATION OF LB-Fabp mRNA[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 376-384.
Citation: CHEN WenBo, LIU LiLi, LI WenSheng, LIN HaoRan. ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM HEPATOPANCREATIC cDNA LIBRARY OF COMMON CARP(CYPRINUS CARPIO) AND CHARACTERIZATION OF LB-Fabp mRNA[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 376-384.
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ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM HEPATOPANCREATIC cDNA LIBRARY OF COMMON CARP(CYPRINUS CARPIO) AND CHARACTERIZATION OF LB-Fabp mRNA

  • State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275

Funds: 

The National Basic Research Program (973 program, No.2004CB117402)

  • Received Date: May 21, 2008
  • Rev Recd Date: January 11, 2009
  • Published Date: May 24, 2009
    Graphical Abstract
    • Abstract
  • A representative cDNA library obtained from hepatopanereas of common carp (Cyprinus carpio) was constructed, and 1016 expressed sequence tags were generated assembling into 115 contiguous sequences and 282 singletons. 215 (54.15%) of the assembled sequences were annotated based upon matching known genes in the GenBank public Databas-es. Functional categorization and comparative analyses of the sequence provided gene identities, expression information and a solid base for further research on common carp hepatopancreas. High expression of liver basic fatty acid binding protein (Lb-Fabp) (11/1016 ESTs) in the library was detected. Two types of Lb-Fabpa and Lb-Fabpb had an open reading frame of 381 bp encoding 126 amino acids, mRNA expression of Lb-Fabps in embryos, larva and adult common carp tissues were determined using semi-quantitative RT-PCR followed by Southern Blot analysis. Lb-Fabp mRNA was expressed with high levels in hepatopancreas, middle intestine and posterior intestine. Low levels of Lb-Fabp mRNA expression were observed in testis and skin, while much lower levels in muscle, brain, ovary, kidney, spleen, gill and heart. No Lb-Fabp mRNA expression was observed in fat and anterior intestine. Lb-Fabp mRNA was first observed in neurula stage, and expressed higher in the following stages. The expression pattern of Lb-Fabp mRNA suggests that common carp Lb-FABPs play an im-portant role in lipid metabolism after the morphogenesis of hepatopancreas and intestine.
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    • References (26)
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