Volume 32 Issue 1
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ZHENG Jin-Song, LIAO Xiao-Lin, TONG Jin-Gou, WANG Ding. A PILOT STUDY ON ISOLATION OF MICROSATELLITE DNA IN THE YANGTZE FINLESS PORPOISE (NEOPHOCAENA PHOCAENOIDES ASIAEORIENTALIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(1): 19-25.
Citation: ZHENG Jin-Song, LIAO Xiao-Lin, TONG Jin-Gou, WANG Ding. A PILOT STUDY ON ISOLATION OF MICROSATELLITE DNA IN THE YANGTZE FINLESS PORPOISE (NEOPHOCAENA PHOCAENOIDES ASIAEORIENTALIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(1): 19-25.
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A PILOT STUDY ON ISOLATION OF MICROSATELLITE DNA IN THE YANGTZE FINLESS PORPOISE (NEOPHOCAENA PHOCAENOIDES ASIAEORIENTALIS)

  • Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039

  • Received Date: October 24, 2005
  • Rev Recd Date: July 19, 2007
  • Published Date: January 24, 2008
    Graphical Abstract
    • Abstract
  • To the aim of developing specific microsatellite markers for the endangered Yangtze finless porpoise Neophocaena pho-caenoides asiaeorientalis,72 microsatellite DNA sequences were isolated using an improved FIASCO ( Fast Isolation by AFLP ofSequences Containing Repeats) protocol.According to Weber’s classification rules,these sequences were divided into three cate2gories: perfect repeat sequences ( 58.3 % of total ), imperfect repeat sequences ( 22.2 %), and compound repeat sequences(19.5 %). Thirty PCR primer pairs were designed and synthesized,and then screened by a sample panel of 12 random selectedYangtze finless porpoises.As a result,14 of themwere proved to be polymorphic exhibiting 2 to 13 alleles (mean 5.86 alleles/ lo-cus). Their average observed heterozygosity and expected heterozygosity were 0.560 and 0.709 respectively. These are the firstbatch of specific microsatellite markers for the Yangtze finless porpoise,and they should play great role in succedent conservationgenetics studies.
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    • References (40)
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