AMMONIA EXCRETION AND HEAMOLYMPH NITROGENOUS CONTENTS OF MITTEN CRAB ( ERIOCHEIR SINENSIS) AT DIFFERENT ENVIRONMENTAL pH

YU Min; WANG Shun-Chang; LU Yun

Volume 32 Issue 1

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2008 > 32(1): 62-67.

YU Min, WANG Shun-Chang, LU Yun. AMMONIA EXCRETION AND HEAMOLYMPH NITROGENOUS CONTENTS OF MITTEN CRAB ( ERIOCHEIR SINENSIS) AT DIFFERENT ENVIRONMENTAL pH[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(1): 62-67.

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AMMONIA EXCRETION AND HEAMOLYMPH NITROGENOUS CONTENTS OF MITTEN CRAB ( ERIOCHEIR SINENSIS) AT DIFFERENT ENVIRONMENTAL pH

School of Life Science, Anhui University, Anhui 230039;
2. Department of Chemistry and Biology, the Normal College of Huainan, Huainan 232001

Received Date: December 19, 2006
Rev Recd Date: April 25, 2007
Published Date: January 24, 2008

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Abstract

Abstract

Crustacean nitrogenous excretion was easily affected by environmental factors, such as ambient ammonia,salinity andpH. In order to investigate the effects of pH on ammonia excretion and heamolymph nitrogenous contents of Chinese mitten crab( Eriocheir sinensis),matured female crabs were exposed to pH 415,610,715,910 and 1015 for 24h,the ammonia excretion rateand haemolymph nitrogenous contents were determined after exposure. The results showed that there was no significant differenceof the ammonia excretion of E. sinensis frompH 415 to pH 910,but the inhibit effects on the ammonia excretion were observed atpH 1015 ( p < 0105). The haemolymph pH kept relatively constant at the levels of (7179 ±0102) - (7182 ±0101) frompH 415to pH 910, but increased significantly to 8. 2 ±0. 05 when exposed to pH 1015 ( p < 0105). The content of haemolymphammonia,urea and urate were also affect by environmental pH. The content of haemolymph ammonia increased from (4106 ±012)μg/ mL at pH 715 to (5190 ±0161)μg/ mL at pH 1015 and decreased to (1186 ±013)μg/ mL at pH 415. The haemolymph ureaand urate also affected by environmental pH,the content of urea and urate increased from (119 ±0112)μmol/ mL and (01139 ±6 6 水生生物学报 32卷01003)μmol/ mL at pH 715 to (2147 ±0114)μmol/ mL and (01172 ±01012)μmol/ mL at pH 1015, respectively.At pH 415,theconcentrations of haemolymph were (1181 ±0108)μmol/ mL and (01096 ±01002)μmol/ mL,significantly lower than that of pH715. The high pH exposure also affected the metabolisms of protein and amino acids. The total haemolymph protein and haemo2cyanin concentrations exhibited unchanged from pH 415 to pH 910,but decreased significantly at the pH 1015. The content ofhaemolymph protein and haemocyanin were (76194 ±3108) mg/ mL and (0189 ±0101) mmol/ mL at pH 715, decreased to(57118 ±8149) mg/ mL and (0153 ±0103) mmol/ mL at pH 1015,respectively. In contrast, the total haemolymph free aminoacids increased significantly at pH 1015 ( p < 0105). The results indicated that exposure E. sinensis to high environmental pHcaused decrease in ammonia excretion rates,and elevated the contents of haemolymph ammonia,urea,urate and total free aminoacids,which might be contributed to the detoxification of ammonia.
- Eriocheir sinensis,
- pH,
- Ammonia nitrogen,
- Haemolymph

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[1]	Wen X B,Chen L Q,Ai C X,Zhou Z L. Effects of temperature andbody weight on fasting metabolism of the juvenile Eriocheir sinensis[J]. Acta Hydrobiologia Sinica,2003,27 (4):357-361 [温小波,陈立侨,艾春香,周忠良. 温度和体重对中华绒螯蟹幼蟹饥饿代谢的影响. 水生生物学报,2003,27 (4):357-361]
[2]	Boyd C E.Water quality in ponds for aquaculture [M].Birmingham:Birmingham Publishing AL. 1990,482
[3]	Feng YQ. Summary of acid rain’s S482tatus,causes of natural forma2tion and counter measures research in China [J]. Yunnan GeographicEnvironment Research,2004,16 (1):25-28 [冯砚青. 中国酸雨状况和自然成因综述及防治对策探究. 云南地理环境研究,2004,16 (1):25-28] 1 期于敏等:中华绒螯蟹在不同pH下氨氮排泄和血淋巴含氮成分的变化 65
[4]	Zanotto F P,Wheatly M G. The effect of ambient pH on electrolyteregulation during the postmoult period in freshwater crayfish Procam2barus clarkia [J]. J Exp Biol,1993,178:1-19
[5]	Allan GL,Maguire GB. Effects of pH and salinity on survival,growthand osmoregulation in Penaeus monodon Fabricius [J]. Aquaculture,1992,107:33-47
[6]	Morgan D O,McMahon B R. Acid tolerance and effects of sublethalacid exposure on ion regulation and acid2base status in two crayfishProcambarus clarkii and Orconectes rusticus [J]. J Exp Biol, 1982,97:241-252
[7]	Jin S D,Zhao X H. Influence of low temperature,salinity2lowering andpH on metamorphosis of Chinese mitten2handed crab [J]. Journal ofDalian Fisheries University,2001,16:249-256 [金送笛,赵晓红.低温、降盐度及pH 对中华绒螯蟹幼体变态的影响. 大连水产学院学报,2001,16:249-256]
[8]	Regnault M. Nitrogen excretion in marine and freshwater Crustacea[J]. Biol Rev,1987,62:1-24
[9]	Wright P A. Nitrogen excretion:three end products,many physiologi2cal roles [J]. J Exp Biol,1995,198:273-281
[10]	Pequeux A,Gilles R. Na +fluexes across isolated perfused gills of theChinese crab Eriocheir sinensis [J]. J Exp Biol,1981,92:173-186
[11]	Chen J C, Kou C T. Nitrogenous excretion in Macrobrachium rosen2bergii at different pH levels [J]. Aquaculture,1996,144:155-164
[12]	Schmitt A S C,Santos E A. Haemolymph nitrogenous constituents andnitrogen efflux rates of juvenile shrimp, Penaeus paulensis ( PereZ2Farfante), exposed to ambient ammonia2N [J]. Aquature Research,1999,30:1-11
[13]	Lee W C,Chen J C. Hemolymph ammonia,urea and uric acid levelsand nitrogenous excretion of Marsupenaeus japonicus at different salin2ity levels [J]. J Exp Mar Biol Ecol,2003,288:39-49
[14]	Chen J C,Chen C T,Cheng S Y. Nitrogen excretion and changes ofhemocyanin, protein and free amino acid levels in the hemolymph ofPenaeus monodon exposed to ambient ammonia2N at different salinitylevels [J]. Mar Ecol Prog Ser,1994,110:85-94
[15]	Nickerson K W,Van Holde K E. A comparison of molluscan andarthropod hemocyanin. I. Circular dichroism and absorption spectra[J]. Comp Biochem Physiol,1971,39B:855-872
[16]	Weihrauch D,Becker W,Postel U, et al. Active excretion of ammoniaacross the gills of the shore crab Carcinus maenas and its relation toosmoregulatory ion uptake [J]. J Comp Physiol,1998,168B:364-376
[17]	Kormanik GA,Cameron J N. Ammonia excretion in the seawater bluecrab ( Callinectes sapidus ) occurs by diffusion, and not Na + / NH+4exchange [J]. J Comp Physiol,1981,141B:457-462
[18]	Wright P A,Wood CM. An analysis of branchial ammonia excretion inthe freshwater rainbow trout:Effects of environmental pH change andsodium uptake blockade [J]. J Exp Biol,1985,114:329-353
[19]	Hanlon D P. The distribution of arginase and urease in marine inverte2brates [J]. Comp Biochem Physiol,1975,52B:261-264
[20]	Chen J C,Chen J M. Arginase specific activity and nitrogenous excre2tion of Penaeus japonicus exposed to elevated ambient ammonia [J].Mar Ecol Prog Ser,1997,153:197-202
[21]	Regnault M. Effect of air exposure on nitrogen metabolism in the crabCancer pagurus [J]. J Exp Zool,1992,264:372-380
[22]	Linton S M,Greenaway P. Urate deposits in the gecarcinid land crabGecarcoidea natalis are synthesised de novo from excess dietary nitro2gen [J]. J Exp Biol,1997,200:2347-2354
[23]	Durand F,Chausson F,Regnault M. In creases in tissue free aminoacid levels in response to prolonged emersion in marine crabs:an am2monia2detoxifying process efficient in the intertidal Carcinus maenusbut not the subtidal Necora puber [J]. J Exp Biol,1999,202:2191-2202

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AMMONIA EXCRETION AND HEAMOLYMPH NITROGENOUS CONTENTS OF MITTEN CRAB ( ERIOCHEIR SINENSIS) AT DIFFERENT ENVIRONMENTAL pH

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