RESPONSES OF GLUTATHIONE CYCLE ENZYM ES AND GLUTATHIONE M ETABOL ISM TO 1-HEXYL-3-M ETHYL IM IDAZOL IUM STRESS IN SCENEDESMUS OBL IQUUS

MA Jian-Min; CAI Lin-Lin; HU Ling-Wei; JIN Tong-Xia; LI Xiao-Yu; WANG Jian-Ji

Volume 33 Issue 4

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2009 > 33(4): 696-701.

MA Jian-Min, CAI Lin-Lin, HU Ling-Wei, JIN Tong-Xia, LI Xiao-Yu, WANG Jian-Ji. RESPONSES OF GLUTATHIONE CYCLE ENZYM ES AND GLUTATHIONE M ETABOL ISM TO 1-HEXYL-3-M ETHYL IM IDAZOL IUM STRESS IN SCENEDESMUS OBL IQUUS[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(4): 696-701.

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RESPONSES OF GLUTATHIONE CYCLE ENZYM ES AND GLUTATHIONE M ETABOL ISM TO 1-HEXYL-3-M ETHYL IM IDAZOL IUM STRESS IN SCENEDESMUS OBL IQUUS

Received Date: October 15, 2008
Rev Recd Date: April 01, 2009
Published Date: July 24, 2009

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Abstract

The effectsof ionic liquid 1-hexyl-3-methylimidazolium ([C6mim ]Br), with the concentrationsof 0, 1, 5, 10, 15 and 20 mg/L and exposed for the 24, 48, 72 and 96h respectively, on glutathione (GSH) and the enzymes in glutathione metabolism of Scenedesmus obliquus such as glutathione peroxidase (GPX), glutathione S-transferases (GST) and glutathione reductase (GR) were studied in the present study1 The results indicated that the GSH contents decreased with the concentration of [C6mim ]Br increasing at the 24, 48 and 72h, but it did not change when [C6mim ]Br = 1mg/L. The GSH contentswere similar to or lower than that of the control level at the 96h. The activities of GPX and GST increased markedly before 72h except for the activity of GST at the highest concentration (20 mg/L), but they both decreased to the control level at the 96h1 The activities of GR increased at the concentration of 1 mg/L and then decreased with the concentration increasing at the 24h, increased to the control level at the 48h, and then reached its peak at the 72h (when [C6mim ]Br≥10 mg/L the GR activities exceeded the control level), but at the 96h, it decreased markedly even weaker than the control level. GR was the enzyme that limited the reaction velocity in the system of glutathione cycle and the GST was the most active and sensitive enzyme. It could be used as sensitive biomarkers under the stress induced by [C6mim]Br. [C6mim ]Br induced oxidative stress of S. obliquus at the concentration of 1 mg/L and had environmental toxicity.
- Ionic liquid,
- Scenedesmus obliquus,
- Glutathione,
- Glutathione S-transferases,
- Glutathione peroxidase,
- Glutathione reductase

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[1]	Brennecke J F, Maginn E J. Ionic Liquids: Innovative Fluids for Chemical Processing [J]. AIChE J, 2001, 47: 2384-2389
[2]	Swatloski R P, Holbrey J D, Memon S B, et al. Using Caenorhabditis elegans to probe toxicity of 12alkyl232methylimizolium chloride based ionic liquids [J]. Chem Commun,2004,668-669
[3]	Stepnowski P, SkladanowskiA C, Ludwiczak A, et al. Evaluating the cytotoxicity of ionic liquids using human cell line Hela [J]. Human Exp Toxicol, 2004, 23: 513-517
[4]	Ranke J, M?lter K, Stock F, et al. Biological effects of imidazolium ionic liquidswith varying chain lengths in acuteVibrio fischeri andWST21 cell viability assays [J]. Ecotoxicol Environ Saf, 2004, 58: 396-404
[5]	Docherty KM, Kulpa C F Jr. Toxicity and antimicrobial activity of imidazolium and pyridinium ionic liquids [J]. Green Chem, 2005, 7: 185-189
[6]	Couling D J, Bernot R J, Docherty K M, et al. Assessing the factors responsible for ionic liquid toxicity to aquatic organisms via quantitative structure2property relationship modeling [J]. Green Chem, 2006, 8: 82-90
[7]	EN. Water quality, freshwater algal growth2inhibition tests with Suenedesmus subspicatus and Selenastrum capricornutum (ISO 10253, 1993). European Committee for Standardization, Brussels
[8]	KobraeiM E, White D S. Effects of 2, 42D ichlorohenoxyacctic acid on Kentucky algae: simultaneous laboratory and field toxicity testings [J]. Arch Environ Contam Toxicol,1996, 31:571-580
[9]	LewisM A. Use of freshwater plants for phytotoxicity testing: a review [J]. Environ Pollut, 1995, 87: 319-336
[10]	Ma J Y, Zheng R, Xu L, et al. Differential sensitivity of two greenalga, Scenedesmus obliquus and Chlorella pyrenoidosa to 12 pesticides [J]. Ecotoxicol Environ Saf, 2002, 52: 57-61
[11]	XiongL, Wu ZB, Kuang Q J, et al. Studies on the toxicity of cypermethrin to Scenedesmus obliquus [J]. Acta Hydrobiologica Sinica, 2002, 26 (1) : 66-73 [熊丽, 吴振斌, 况琪军, 等.氯氰菊酯对斜生栅藻的毒性研究. 水生生物学报, 2002, 26 (1) : 66-73]
[12]	Rennenberg H, Brunold C. Significance of glutathione metabolism in plants under stress [J]. Prog Bot, 1994, 55: 142-156
[13]	Venugopal N B R K, Ramcsh T V D D, Reddy D S, et al.Effect of cadmium on antioxidant enzyme activities and lipid peroxidation in a freshwater field crab, Barytelphisa nuerini [J]. Bull Environ Contam Toxicol, 1997, 59: 132-138
[14]	Gensemer R W, Dixon D G, Greenberg B M. Using chlorophyll a fluorescence to detect the onsetof anthracene photoinduced toxicity in Lemna gibba, and the mitigating effects of a commercial humic acid [J]. LimnolOceanogr, 1999, 44 (322) : 878-888
[15]	ParrL B, Perkins R G, Mason C F, Reduction in photosynthesis efficiency of Chladophora glomerata induced by overlying canopies ofLemna sp. [J]. W ater Res, 2002, 36: 1735-1742
[16]	Pernak J, Goc I, Mirska I. Antimicobial activitiesof protic ionic liquids with lactate anion [J]. Green Chem,2004, 6:323-329
[17]	Cho CW, Jeon Y C, Pham T P T, Vijayaraghavan K, Yun Y S. The ecotoxicity of ionic liquids and traditional organic solvents on microalga Selenastrum capricornutum [J]. Ecotoxicol Environ Saf, 2008, 71: 166-171
[18]	Bonh?te P, DiasA P, Papageorgiou N, et al. Hydrophobic highly conductive anbient2temperaturemolten salts [J]. Inorg Chem,1996, 35: 1168-1178
[19]	FawellJ K, MitchellR E, EverettD J, et al. The toxicityof cyanobacterial toxins in the mouse: I. Microcystin-LR [J]. Human Exp Toxicol, 1999, 18: 162-167
[20]	BradfordM N. A rapid and sensitive method for the quantitation of microgram quantitiesof protein utilizing the principle of protein dye2binding [J]. Anal B iochem, 1976, 72: 248-255
[21]	Pang Z J, ZhouM, Chen Y. Researchmethod of free radical iatrology [M]. Beijing: People Sanitation Press. 2000, 168-169[庞占军, 周玫, 陈媛. 自由基医学研究方法. 北京: 人民卫生出版社. 2000, 168-169]
[22]	Li C X, Jiang L N, Shao Y, et al. Biostatistics (the third edition) [M]. Beijing: Science Press. 2005, 100 [李春喜, 姜丽娜, 邵云, 等. 生物统计学 (第三版). 北京: 科学出版社.2005, 100]
[23]	PotterD W, Tran TB. Apparent rates of glutathione turnover in rat tissues [J]. ToxicolAppl Pharmacol, 1993, 120: 186-192
[24]	Nagalakshmi N, Prasad M N V. Responses of glutathione cycleenzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus [J]. Plant Science, 2001, 160: 291-299
[25]	Zirong X, Shijun B. Effects ofwaterborne Cd exposure on glutathione metabolism in Nile tilapia (Oreochrom is niloticus) liver[J]. Ecotoxicol Environ Saf, 2007, 67: 89-94
[26]	Sen C K. Glutathione homeotasis in response to exercise training and nutritional supplements [J]. M ol CellB iochem, 1999, 196:31-42
[27]	Wang Y, Chen Y G, Ge Z Z, et al. Influence of TBT on ROS, antioxidant enzymes and detoxification system enzyme in rat liver[J]. China Environmental Science, 2005, 25 (4) : 428-431[王燕, 陈永刚, 葛郑增,等. TBT对大鼠 ROS、抗氧化酶和解毒系统酶的影响. 中国环境科学, 2005, 25 (4) : 428-431]
[28]	JiL, Stratman FW, LardyH A. Enzymatic down regulation with exercise in rat skeketal muscke [J]. Arch B iochem B iophys,1988, 263: 137-145
[29]	Haberland A, Mader K, StosserR, et al. Comparison ofmalondialdchydc and hydrogen peroxide modified CuZnSOD by EPR spectroscopy [J]. Agents Actions, 1993, 40: 166-170
[30]	Ma JM, CaiL L, Jin TX, et al. Effectsof 1-hexyl-3-methylimidazolium on antioxidant enzyme activities and liquid peroxidation of Scenedesmus obliquus [J]. Journal of Wuhan Botanical Research, 2008, 26 (5) : 509-513 [马剑敏,蔡林林,靳同霞,等. 溴化 12己基 232甲基咪唑对斜生栅藻抗氧化酶和膜脂过氧化的影响. 武汉植物学研究, 2008, 26 (5) : 509-513]

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RESPONSES OF GLUTATHIONE CYCLE ENZYM ES AND GLUTATHIONE M ETABOL ISM TO 1-HEXYL-3-M ETHYL IM IDAZOL IUM STRESS IN SCENEDESMUS OBL IQUUS

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