WANG Qiu-Rong, Toshio Takeuchi, Hirofumi Furuita. STUDIES ON THE ROLE OF PEPTIDASE AND POWDER SOYBEAN LECITHIN IN MICROPARTICLE DIETS FOR RED SEA BREAM PAGRUS MAJOR LARVAE[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(6): 734-741.
Citation: WANG Qiu-Rong, Toshio Takeuchi, Hirofumi Furuita. STUDIES ON THE ROLE OF PEPTIDASE AND POWDER SOYBEAN LECITHIN IN MICROPARTICLE DIETS FOR RED SEA BREAM PAGRUS MAJOR LARVAE[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(6): 734-741.

STUDIES ON THE ROLE OF PEPTIDASE AND POWDER SOYBEAN LECITHIN IN MICROPARTICLE DIETS FOR RED SEA BREAM PAGRUS MAJOR LARVAE

Funds: 

The project sponsored by Taiyo Yushi Co., Ltd.Yokohama, Japan

  • Received Date: December 19, 2005
  • Rev Recd Date: May 19, 2006
  • Published Date: November 24, 2006
  • This study investigates the effects of exogenous enzyme supplementation on the digestibility of microparticle diets (MD). Powder soybean lecithin and gluten were used instead of paste soybean lecithin for evaluating its effect on the leaching rate inMD. Three kinds of MD (MD-T;MD-S;MD-U)were prepared.Based on formulation of MD-S, MD-T included powder soybean lecithin instead of paste soybean lecithin and gluten as a binder.MD-U was supplied with 0.1 % of peptidase.The other ingredients were the same in the three diets.Within the first 15 minutes of submersion in water, the leaching rate for MD-T (35.5%) was lower than for MD-S (46.8 %)and MD-U (45.8%).The first feeding red sea bream larvae were fed the three above mentioned MD and live food (LF)as a control.The trial lasted until 20 days after hatching.Final survival rate was highest in LF treatment (86.3%), followed by MD-T(20.7 %)treatment, which was significantly higher (P <0.05)than the results for MDS (13.3%)andMD-U(13.6 %)treatments.Final total length (TL)of larvae fed LF was with 6.14 ±0.49mm significantly larger (P <0.05)than for those fed withMD, which reached at sizes ranged from 4.23 ±0.30mm (MD-S)to 4.46 ±0.30mm (MD-T).There were no significant differences in growth among MD treatments.Histological analysis of digestive epithelium of larvae showed thick and well-developed intestine folds at 12 days after hatching in both of LF andMD treatments.However, the intestine epithelium of larvae fed onMD appeared thin and most cell desquamation were observed at 18 days after hatching, indicating limited digestive capacity after 12 days after hatching and led to sharp decline of survival rate inMD treatments thereafter.Daily increments for protein, DNA and RNA as well as the ratio of RNA/DNA were higher in larvae fed LF compared to the respective values obtained for those fedMD.Larvae fedMD-T showed higher value of the above parameters than those fedMD-S andMD-U, suggesting larvae had a better nutritional condition in MD-T treatment than those inMD-S andMD-U treatments.The results indicated that supplementation of peptidase could not facilitate the digestion of MD for red sea bream larvae.However, using powder soybean lecithin and gluten could reduce the leaching rate from MD and consequently improved larval survival and growth.
  • [1]
    Cousin J C B, Baudin-Laurencin F, Gabaudan J.Ontogeny of enzymatic activiti es in fed and fasting turbot, Scophtalmus maximus L[J].J.Fish Biol., 1987, 30 :15-33
    [2]
    Wang C, Xie S.Advances in nut rition and feed for postlarvae and juveniles[J].Acta Hydrobiologica Sinica, 2004, 28(5):557-562
    [3]
    Lauff M, Hofer R.Proteolytic enzymes in fish development and theimportance of di etary enzymes [J].Aquaculture, 1984, 37 :335-346
    [4]
    Kolkovski S, Tandler A, Kissi l G, Gertler, A.The effect of dietaryexogenous digestive enzymes on ingestion assimilation, growth andsurvival of gi lthead seabream Sparus aurata, Sparidae, Linnaeus larvae[J].Fish Physiol.Biochem., 1993, 12 :203-209
    [5]
    Pedersen B H, Hjelmeland K.Fate of t rypsin and assimilation efficiencyin larval herring Clupea harengus following digestion of copepods[J].Mar.Biol., 1988, 97 :467-476
    [6]
    Zambonino-Infante J L, Cahu C L, Peres A, Quazuguel P, Le GallM.M.Sea bass Dicentrarchus labrax larvae fed different Artemia ratios :growth, pancreas enzymatic response and development of digestive functions [J].Aquaculture, 1996, 139 :129-138
    [7]
    Cahu C, Zambonino-Infante J L.Is the digestive capacity of marinefish larvae sufficient for compound diet feeding ? Aquacult.Int.,1997, 5:151-161
    [8]
    Kurokawa T, Shiraishi M, Suzuki T.Qualification of exogenous protease derived from zooplankton in the intestine of Japanese sardineSardinops melanoticus larvae [J].Aquaculture, 1998, 161 :491-499
    [9]
    Linder P, Eshel A, Kolkovski S, Tandler A, Harpaz S.Proteolysisby juvenile sea bass Dicentrarchus labrax gastrointest inal enzymes as amethod for the evaluation of feed proteins [J].Fish Physiol.Biochem., 1995, 14 :399-407
    [10]
    Carter C G, houlihan D F, McCarthy I A.Feed utilization efficienciesof At lanti c salmon Salmosalar L.part:effect of a single supplementaryenzyme [J].Comp.Biochem.Physiol., 1992, 101 :369-374
    [11]
    Carter C G, houlihan D F, Buchanan B, McCarthy L A.Growth andfeed utilization efficiencies of seawater At lanti c salmon Salmo salarL.fed a diet containing supplementary enzymes [J].Aquacult.Fish.Manage.1994, 25 :37-46
    [12]
    Kolkovski S, Tandler A, Izquierdo M S.Effect of live food, dietarydigestive enzymes on the effi ciency of microdiets for seabass Dicentrarchuslabrax larvae [J].Aquaculture, 1997, 148 :313-322
    [13]
    Kolkovski S, Yackey C, Czeny S, Dabrowski K.The effect of microdietssupplementation of dietary digestive enzymes and hormones ongrowth and enzyme activity in yellow perch juveniles [J].NorthAmeric.J.Aquacult., 2000, 62 :130-134
    [14]
    Ló pez-Alvarado J, Langdon C J, Teshima S I, Kanazawa A.Effect ofcoating and encapsulation of crystalline amino acids on leaching inlarval feeds [J].Aquaculture, 1994, 122 :335-346
    [15]
    Takeuchi T, Wang Q R, Furuita H, Hirota T, Ishida S, HayasawaH.Development of microparticle diets for Japanese f lounder Paralichthysolivaceus larvae [J].Fish.Sci., 2003, 69:547-554
    [16]
    Ogino C, Yang G Y.Requirement of rainbow trout f or diet ary zinc[J].Nippon S uisan Gakkaishi, 1978, 44:1015-1018
    [17]
    Ogino C, Takeuchi L, Takeda H, Watanabe T.Avai lability of dietaryphosphorus in carp and rainbow trout [J].Nippon SuisanGakkaishi, 1979, 45 :1527-1532
    [18]
    Wang Q R,Takeuchi T, Hirota T, Ishida S, Miyakawa H, HayasawaH.Application of microparticle diets for Japanese flounder Paralichthysolivaceus larvae [J].Fish.Sci., 2004, 70:611-619
    [19]
    Nakano H.Techniques for studying on the early life history of fi shes-13.Techniques for quantifying nucleic acid in fish larvae and juveniles[J].Aquabiology, 1988, 54 :23-26 (in Japanese)7 40 ACTA HYDROBIOLOGICA SINICA 30卷
    [20]
    Tanangonan J B, Nakano H, Tanaka M.Changes in DNA, RNA,and protein content during early growth and development of Japanesef lounder, Paralichthys olivaceus [J].Suisanzoshoku, 1998, 46(2):243-252
    [21]
    Bogut I, Opacak A, Stevic I.The influence of polyzymes added tothe food on the growth of carp fingerlings Cyprinus carpio [J].Aquaculture, 1995, 129 :252
    [22]
    Lee P S, Southgate P C, Fielder D S.Assessment of two mi croboundartificial diets for weaning Asian sea bass Lates calcarifer [J].AsianFish.Sci., 1996, 9 :115-120
    [23]
    Walford J, Lim T M, Lam T J.Replacing live foods with microencapsulateddiets in the rearing of seabass Lates calcarifer larvae :dothe larvae ingest and digest protein-membrane microcapsule ? [J].Aquaculture, 1991, 92 :225-235
    [24]
    Gwak W S, Tanaka M.Developmental change in RNA :DNA ratios offed and starved laboratory-reared Japanese f lounder larvae and juveniles,and its application to assessment of nutritional condition forwild fish [J].J.Fish Biol.2001, 59 :902-915
    [25]
    Gwak W S, Tsusaki T, Tanaka M.Nutritional condit ion, as evaluatedby RNA/ DNA ratios, of hatchery-reared Japanese flounder fromhatch to release [J].Aquaculture, 2003, 219:503-514
    [26]
    Summerf elt R C, Hall G E.The Age and growth of fi sh, Iowa:TheIowa State University Press.1987, 45-64
    [27]
    Buckley L J.RNA-DNA ratio :an index of larval f ish growth in thesea [J].Mar.Biol., 1984, 80 :291-298
    [28]
    Westerman M, Holt G J.RNA :DNA ratio during the critical periodand early larval growth of red drum Sciaenops ocellatus [J].Mar.Biol.1994, 121 :1-9
    [29]
    Hovenkamp F, Witte J J.Growth, otolith growth and RNA :DNA ratios of larval plaice Pleuronectes platessa in the north sea 1987 to 1989[J].Marine Ecology Progress Series, 1991, 70 :105-116
    [30]
    Clemmesen C, Doan T.Does otolith structure reflect the nutritionalcondition of a fish larva?Comparison of otolith structure and biochemical index (RNA :DNA ratio)determined on cod larvae [J].MarineEcology Progress Series, 1996, 138:33-39
    [31]
    Folkvord A, Ystanes L, Johannessen A, Moksness E.RNA :DNAratios and growth of herring (Clupea harengus) larvae reared inmesocosms [J].Mar.Biol., 1996, 126 :591-602
    [32]
    Takii K, Nakamura M, Takaoka O, Furuta S I, Kumai H.Nucleicacid content and chemical composition of red sea bream, from larvaejust after hat ching to juveniles [J].Suisanzoshoku, 1992, 40 (3):285-290
    [33]
    Sato C, Kimura R, Nakata K, Umeda S, Suzuki M.RNA/DNA ratio of first-feeding larvae of Japanese Sardine [J].Fish.Sci.,1995, 61 :538-539
    [34]
    Clemmesen C.The effect of food availability, age or size on theRNA :DNA rat io of individually measured herring larvae :laboratorycalibrat ion [J].Mar.Biol., 1994, 118 :377-382

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