Song Ping, Li Xiaoying, Xiong Quanwei. A MAP OF RESTRICTION ENDONUCLEASES OF MEGELOBRAMA AMBLYCEPHALA YIH[J]. ACTA HYDROBIOLOGICA SINICA, 1996, 20(2): 119-126.
Citation: Song Ping, Li Xiaoying, Xiong Quanwei. A MAP OF RESTRICTION ENDONUCLEASES OF MEGELOBRAMA AMBLYCEPHALA YIH[J]. ACTA HYDROBIOLOGICA SINICA, 1996, 20(2): 119-126.
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A MAP OF RESTRICTION ENDONUCLEASES OF MEGELOBRAMA AMBLYCEPHALA YIH

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    • Abstract
  • The mitochondrial DNA (mtDNA) of Megelobrama amblycephala were digested by 11 restriction endonucleases: BamH Ⅰ, Bgl Ⅰ, Bgl Ⅱ, EcoR Ⅰ, Hpa Ⅰ, Kpn Ⅰ, Pst Ⅰ, Sac Ⅰ,Sal Ⅰ, Xba Ⅰ, Xho Ⅰ into different fragment: 2, 3, 2, 3, 3, 1, 0, 1, 0, 0, 0 respectively. Estimated by agarose gel electrophoresis, the size of mtDNA from Megelobrama amblycephala is 16020 ±356 bp. When comparing the double restriction fragments with standard molecular weight of Mark Ⅰ (Hind Ⅲ digested λDNA) and Mark Ⅱ(EcoR Ⅰand Hind Ⅲ digested λDNA), we constructed the enzymes physical map.
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