HUANG Yan-Yan, OUYANG Shan, WU Xiao-Ping, LIU Huan-Zhang. PHYLOGENY OF THE UNIONIDAE BASED ON PARTIAL MITOCHONDRIAL 16S rRNA SEQUENCES[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(3): 258-263.
Citation: HUANG Yan-Yan, OUYANG Shan, WU Xiao-Ping, LIU Huan-Zhang. PHYLOGENY OF THE UNIONIDAE BASED ON PARTIAL MITOCHONDRIAL 16S rRNA SEQUENCES[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(3): 258-263.

PHYLOGENY OF THE UNIONIDAE BASED ON PARTIAL MITOCHONDRIAL 16S rRNA SEQUENCES

  • Received Date: January 20, 2002
  • Rev Recd Date: March 26, 2002
  • Published Date: May 24, 2003
  • This study presents the mitochondrial 16S rRNA partial sequences representing 13 genera of Chinese freshwater mussel Unionidae. Alignments were conducted with the help of Clustal X.After alignment,there were 305-320 sites. Sequence variations were analyzed with Mega 2.0 software,one hundred and eight sites were variable,among which there were seventy seven phylogenetically informative sites."Pairwise distances" were computed among the 13 genera with Mytilus edulis designated as outgroup. Only one molecular phylogenetic tree was obtained using Neighbore Joining method of Mega 2.0.The bootstrap analyses (1000 replications) were performed to test the confidence of nodes. According to our results,Unionidae recorded from China group to three clades: a basal Hyriopsis + Solenaia + Lamprotula + Ptychorhynchus clade,an Acuticosta + Unio + Ar2conaia + Cuneopsis + Lanceolaria + Schistodesmus + Arconaia calde and an Anodonta + Cristaria clade. Therefore,Chinese unionids should be divided into three subfamilies: Ambleminae( Hyriopsis + Solenaia + Lamprotula + Ptychorhynchus ),Anodontinae( Anodonta + Cristaria ) and Unioninae ( Acuticosta + Unio + Arconaia + Cuneopsis + Lanceolaria + Schistodesmus + Arconaia ),which also demonstrates the existence of Ambleminae in China. Thereinto,the analysis of mitochondrial 16S rRNA indicates that Solenaia and Lepidodesma,which were placed Anodontinae before,should be moved to the subfamily Ambleminae and Unioninae respectively. To more accurately reflect the phylogeny, Hyriopsis, Lamprotula and Ptychorhynchus should be moved to the subfamily Ambleminae.
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    He S P,The phylogeny of the Glyptostrnoid fishes(Teleostei: Siluriformes, Sisoridae)[J]. Cybium,1996,20(2):115-159[2] Wang W,He S P,NAKAJIMA T,The RAPD analysis for cyprinid fish(APHOCYPRIS AND HEMIGRAMMOCYPRIS)[J]. Acta Hydrob.Sinica,2001,25(3):358-361.[王伟,何舜平,中岛经夫.日本产和中国产中华细鲫的RAPD分析. 水生生物学报,2001,25(3):358-361][3] Yi L,Wang W,Li M Y,et al,A study on the geographical differentiation of Rhodeus ocellatus based on RAPD analysis [J]. Acta Hydrobiologica Sinica,2001,25(3):301-304.[易犁,王伟,李墨怡,等.基于RAPD分析的高体(鱼旁)(鱼皮)地理分化研究. 水生生物学报,2001,25(3):301-304][4] Lü X M,Wang Y X,Zhang Y P,Divergence and phylogeny of mitochondrial cytochrome b gene from species in Nycticebus [J]. Zoolog-ical Research. 2001,22(2):93-98.[吕雪梅,王应祥,张亚平. 蜂猴线粒体细胞色素b基因变异特点及系统发育分析. 动物学研究,2001,22(2):93-98][5] Jiang H Y,Lu P H,Li Y M,Study of the relationship among five species of muscicapidae by mtDNA sequence [J]. Heeditas. 2000,22(1):21-24.[姜海英,陆佩洪,李悦民.(翁鸟)科五种鸟线粒体DNA 序列变化与亲缘关系的研究.遗传,2000,22(1):21-24][6] Geller J B,Carlton J T, Powers D A.Interspecific and intrapopulation variation in mitochondrial ribosomal DNA sequences of Mytilus spp. (Bivalvia:Mollusca)[J]. Molecular Marine Biology and Biotechnology,1993,2:44-50[7] Lydeard C,Mulvey M, Davis G M.Molecular systematics and evolution of reproductive traits of North American freshwater unionacean mussels (Mollusca:Bivalvia) as inferred from 16S rRNA gene sequences [J]. Philosophical Transactions of the Royal Society of London,1996,351(1347):1593-1603[8] Auseul F M.Short protocols in Molecular Biology(2nd Ed) [M]. London:John Wiley & Sons.1992[9] Thompson J D,Gibson T J,Plewniak F,et al.The ClustalX windows interface:flexible strategies ed for multiple sequences alignment aided by quality analysis,tools [J]. Nucleic Acids Research,1997,25(24):4876-4882[10] Kumar S,Tamura K,Jakobsen I B, et al.MEGA 2.0:Molecular Evolutionary Genetics Analysis software [M]. Arizona State University,Tempe,Arizona,USA.2001[11] Flesenstein J.Confidence limits on phylogenies:An approach using the bootstrap [J]. Evolution,1985,39:783-791[12] Ortmann A E.Notes upon the families and genera of the Najades [J]. Annals of the Carnegie Museum,1912,8:222-365[13]Hannibal H A.synopsis of the Recent and Tertiary freshwater Mollusca of the California Province,based upon an ontogenic classification [J]. Proceedings of the Malacological Society of London,1912,10:112-211,pls.5-8[14] Haas F.Superfamily Unionacea.In:Treatise on Invertbrate Paleontology,PartN,Mollusca,6(R.C.Moore,ed),Bivalvial:N411-N470[M].Geological Society of America,Inc.,Boulder,Colorado.1969b[15] Heard W H Guckert R H.A re-evalution of the recent Unionacea(Pelecypoda)of North America[J]. Malacologia,1971,10:333-355[16] Liu Y Y,Economic Fauna Sinica-Freshwater Mollusk [M]. Beijing: Science Press 1979,66-116.[刘月英.中国经济动物志-淡水软体动物,北京:科学出版社,1979,66-116][17] Modell H.Das naurliche system der najadern [J]. Archiv für Molluskenkunde,1964,93:71-106[18] Brandt R A M.The non-marine aquatic Mollusca of Thailand[J]. Archiv für Molluskenkunde,1974,105(I-IV):1-423[19] Heude R P.Conchyliogie fluviatile de la province de Nanking [M]. Paris,1875-1886[20] Simpson C T.Synopsis of the naiads,of pearly freshwater mussels[J]. Proceedings of the U.S.National Museum,1990,22:501-1075[21] Haas F.Superfamilia Unionacea[M].In: Das tierrich (H.Wermuth,ed.),1969a,88:Berlin-1,Germany[22] Moore R C.Treatise on invertebrate Palaeontology[M]. Part N.2.Mollusca 6 bivalvia. Geological Soc. Am.Inc.And Univ.Kansas,1969[23] Davis G M. Genetic relationship among some North America Unionidae:sibling species,convergence,and cladistic relationships[J]. Malacologia,1984,25(2):629-648[24] Simpson C T.A descriptive catalogue of the naiades or pearly freshwater mussels[M].Privately published by Bryant Walker,Detroit,Michigan,1914[25] Wu X P,Liang Y L,Wang H Z.A comparative study on glochidial morphology of Unionidae(Bivalvia)I. Unio douglasiae,Cuneopsis pisciulus,Acuticosta Chinensis and Acuticosta ovata [J]. Acta Hydrobiologica Sinica,1999,23(2):141-145.[吴小平,梁彦龄,王洪铸.蚌科钩介幼虫比较形态学研究,I四个种幼虫的形态. 水生生物学报,1999,23(2):141-145][26] Wu X P,Liang Y L,Wang H Z,Ouyang S.A comparative study on glochidial morphology of Unionidae(Bivalvia)Ⅱ. Lanceolata,Lamprotula,Hyriopsis and Cristaria [J]. Acta Hydrobiologica Sinica,2000,24(3):252-256.[吴小平,梁彦龄,王洪铸,欧阳珊. 蚌科钩介幼虫比较形态学研究,Ⅱ六个种幼虫的形态.水生生物学报,2000,24(3):252-256]
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