Volume 31 Issue 6
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
Related Articles
ZHANG Jian-Dong, ZHOU Hui, CHEN Gang, SHI Gang, ANG Bao-Gui, PAN Chuan-Hao, WU Zao-He. OBSERVATION ON DEVELOPMENT OF BLOOD CELLS IN TRACHINOTUS OVATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(6): 780-786.
Citation: ZHANG Jian-Dong, ZHOU Hui, CHEN Gang, SHI Gang, ANG Bao-Gui, PAN Chuan-Hao, WU Zao-He. OBSERVATION ON DEVELOPMENT OF BLOOD CELLS IN TRACHINOTUS OVATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(6): 780-786.
shu

OBSERVATION ON DEVELOPMENT OF BLOOD CELLS IN TRACHINOTUS OVATUS

  • 1.

    Fisheries College, Guangdong Ocean University, Zhanjiang 524025;

  • 2.

    Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524025

  • Received Date: November 05, 2006
  • Rev Recd Date: June 03, 2007
  • Published Date: November 24, 2007
    Graphical Abstract
    • Abstract
  • Blood cells play important roles in the immune activities and circulating system of fish, the development of blood cells in liver, spleen, pronephros and kidney and the composition of blood cells in peripheral blood of Trachinotus ovatus were studied. Trachinotus ovatus (25.9) 28.0 cm BL, weight 420.5) 525.2g) were anatomized after one week rearing in lab, the liver, spleen, pronephros and kidney were obtained and gently smeared on glass slides, the glasses were stained with Wright. s-Giemsa dye and the blood cells in different development stages were observed by light microscope.The percentages of primitive erythrocytes in total blood cells counted of liver, spleen, pronephros and kidney were 0.05%, 3.84%, 0.89% and 0.95%, respectively. The percentages of primitive and immature lymphocytes in total blood cells counted of liver, spleen, pronephros and kidney were 0.15%, 2.85%, 2.72% and 3.21%, respectively. The percentages of primitive and immature granulocytes in total blood cells counted of liver, spleen, pronephros and kidney were 0.24%, 2.16%, 4.94% and 3.19%, respectively. The percentages of primitive and immature monocytes in total blood cells counted of liver, spleen, pronephros and kidney were 0.18%, 0.06%, 0.17% and 0.03%, respectively. The q-test results showed that erythrocytes developed mainly in spleen; lymphocytes developed mainly in kidney, pronephros and spleen; granulocytes developed mainly in pronephros; monocytes developed in liver, pronephros; spleen and kidney. The development and morphological changes of thrombocytes could not be distinctly distinguished in the smears of four tissues.The cell constitution in peripheral blood of Trachinotus ovatus were studied by smear and light microscope observation. On the Wright. s-Giemsa dye stained smears of peripheral blood, erythrocyte and four kinds of leucocyte: lymphocyte, thrombocyte,neutrophil and monocyte were recognized. Monocytes were the least leucocytes, less than 1% of total leucocytes. The percentages of lymphocytes, thrombocytes, and neutrophils in total leucocytes were (52.521.65)%, (38.231.86) %, (9.255.64) %, respectively.
    • FullText(HTML)
    • References (21)
  • [1]
    Zhu Y X, Cao G L. A study on the blood cell of Angui lla japonica[J] .Journal of Aquacul ture, 1997, (4): 18-20 [朱越雄, 曹广力.鳗鲡血液细胞的研究. 水产养殖, 1997, (4): 18-20;
    [2]
    Lin G H, Zhang F W, Hong Y Z, et al. Hematological study of twoyears old silver carp Hypophthalmichthys molirtix and bighead carp,Aristichthys nobilicx [J] .Acta Hydrobiologica Sinica, 1998, 22(1): 9-16 [林光华, 张丰旺, 洪一江, 等. 二龄鲢和鳙血液的比较研究. 水生生物学报, 1998, 22(1): 9-16;
    [3]
    Chen G, ZhouH, Zhang J D, et al. Hemat ological study and observation on development of blood cells in cobia Rachycentron canadum [J]. ActaHydrobiol ogica Sinica, 2005, 29(5), 564-570 [陈刚, 周晖, 张健东, 等. 军曹鱼血液指标及血细胞发生的观察. 水生生物学报, 2005, 29(5), 564-570;
    [4]
    Zhou Y, Guo W C, Yang Z G, et al. Microstructure and ultra-structure of the peripheral blood cells of European eel (Anguilla anguilla)[J] .Acta Zool ogica Sinica, 2002, 48(3): 393-401 [周玉, 郭文场,杨振国, 等. 欧洲鳗鲡外周血细胞的显微和超微结构. 动物学报, 2002, 48(3): 393-401;
    [5]
    Li C L, Cao F J, Liu C W, et al. Observation on development of blood cell in Plectorhinchus cinctus [J] .Journal of tropical oceanography, 2002, 21(3): 7) 15 [李长玲, 曹伏君, 刘楚吾, 等. 花尾胡椒鲷肾脏和脾脏血细胞发育的研究. 热带海洋学报, 2002, 21(3): 7-15;
    [6]
    Li C L, Cao F J, Liu C W, et al. Observation on development of haemocytes in Boleophthalmus pectinirostris [J] .Journal of Fisheries Sciences of China, 2001, 8(1): 10-14 [李长玲, 曹伏君, 刘楚吾,等. 大弹涂鱼血细胞发生的研究. 中国水产科学, 2001, 8(1): 10-14;
    [7]
    Li C L, Cao F J, Liu C W, et al. Microstructure of pepipheral blood cells in Lutjanus russell [J] .Journal of Zhanjiang Ocean University ,2001, 21(3): 5-9 [李长玲, 曹伏君, 刘楚吾, 等. 勒氏笛鲷外周血细胞显微结构的观察. 湛江海洋大学学报。2001, 21(3): 5-9;
    [8]
    Yuan S Q, Zhang Y A, Yao W J, et al. Micro and ultra-structure of peripheral blood cells of the mandarin fish, Siniperca chuatsi (Basilawsky) [J] .ActaHydrobiologica Sinica, 1998, 22(1): 39-47 [袁仕取, 张永安, 姚卫建, 等. 鳜鱼外周血细胞显微和亚显微结构的观察. 水生生物学报, 1998, 22(1): 39-47;
    [9]
    Huang J, Yan G L, Jiang J P, et al. Observation on the microstructure and ultra-structure of the blood cells of Anguilla anguil la [J] 1Journal of Fuj ian Teachers University (Natural Science), 1999, 15(1): 90-94 [黄键, 颜桂利, 江剑平, 等. 欧洲鳗鲡血细胞显微和超微结构的观察. 福建师范大学学报(自然科学版), 1999, 15(1): 90-94;
    [10]
    Chen G, Zhou H, Ye F L, et al. The observation on development of blood cells in american red fish (Sciaenops ocellatus) [J] .Journal of Tropical Oceanography, 2006, 25(2): 60-67 [陈刚, 周晖, 叶富良,等. 美国红鱼血细胞观察. 热带海洋学报, 2006, 25(2): 60-67;
    [11]
    Xiao Y J, Jin Y P, Zheng C F, et al. Observat ion on the peripheral blood cellsmorphology of Mi sgurnus anguillicaudatus [J] .Journal of Fuj ian Teachers University (Natural Science), 2001, 17(4): 93-96[肖义军, 金雅平, 郑翠芳, 等. 泥鳅外周血细胞形态的观察.福建师范大学学报(自然科学版), 2001, 17(4): 93-96;
    [12]
    Chen X Y. Heamocyt ogenesis in southern catfish Silurus meridional is[J] .Journal of Southwest China Normal University (Natural Science), 2000, 25(3): 281-287 [陈晓耘. 南方鲶幼鱼血细胞发生的研究. 西南师范大学学报, 2000, 25(3): 281-287;
    [13]
    Roger B, Bobbie B, Julio C, et al1 A cytochemical, light and electron microscopic study of the peripheral blood leucocytes of hybrid surubim catfish (Pseudoplatyst oma corruscansx Pseudoplatystoma fasciatum)[J] .Comp Clin Path, 2003, 12: 61-68;
    [14]
    Ivet e K, Mizue I, Wilson da Silva, et al1 Cytochemical aspect s of the peripheral blood cells of Oreochromis (Tilapia) niloticus .(Linnaeus,1758) (Cichlidae, Teleost ei)-Part II [J] .Braz .J. Vet. Res .Anim .Sci1, 2001, 38 (6): 273-277;
    [15]
    Lin G H, Zhang FW, Hong Y J, et al1 Comparative hematological studies in Megalobrama amblycephala and Carassius auratus cavieri[J]1 Acta Zoologic Sinica, 1996, 42(3): 260-268 [林光华, 张丰旺, 洪一江, 等. 团头鲂和日本白鲫血液的比较研究. 动物学报, 1996, 42(3): 260-268;
    [16]
    Xiao Y J, Jin Y P, Zheng C F, et al. Observation on the developmentof blood cells in head kidney, spleen and liver of Misgurnus anguillicaudatus[J] .Journal of Fujian Teachers University (NaturalScience), 2001, 17(3): 65-69 [肖义军, 金雅平, 郑翠芳, 等.泥鳅头肾、脾脏和肝脏中血细胞发生的观察. 福建师范大学学报(自然科学版), 2001, 17(3): 65-69;
    [17]
    Zhang H F, Wang Y X, Lin L, et al. Studies on hematic properties and biochemical indices of Epinephelus coi oides [J] .Journal of South China Normal University (Natural Science Edition), 2004, (1): 102-107 [张海发, 王云新, 林蠡, 等. 斜带石斑血液性状及生化指标的研究. 华南师范大学学报(自然科学版), 2004, (1): 102-107;
    [18]
    Gu S Y, Yin N, Zhao Q L, et al. Microstructure of peripheral blood cells of the Fugu obscurus [J] .Reserv oir Fisheries, 2002, 20(6): 1-2 [顾曙余, 殷宁, 赵清良, 等. 暗纹东方外周血细胞的显微结构观察. 水利渔业, 2002, 20(6): 1-2;
    [19]
    Zhou H, Chen G, Zhang J D, et al. Observation on development of blood cells in Epinephelus coioides [J]1 Journal of Guangdong Ocean Universit, 2006, 26(4): 3-7 [周晖, 陈刚, 张健东, 等. 斜带石斑血细胞发生的观察. 广东海洋大学学报, 2006, 26(4): 3-7;
    [20]
    Qiu Y C. Fish toxicity experiment methods to detect water pollution[M] .Beijing: China Environmental Science Press. 1992, 100-104[邱郁春. 水污染鱼类毒性实验方法. 北京: 中国环境科学出版社. 1992: 100-104;
    [21]
    Chen F P. Inspection of haematology [M] .Beijing: People. s Medical Publishing House. 2003: 16-22 [陈方平. 血液学检验. 北京: 人民卫生出版社. 2003: 16-22]
    • Related Articles

  • Related Articles

    [1]HU Shao-Qiu, DUAN Rui, ZHANG Dong-Xu, BAO Jiang-Hui, LÜ Hua-Fei, DUAN Ming. CLASSIFICATION OF 3D POINT CLOUD MODELS OF FISH BASED ON POINT TRANSFORMER APPROACH[J]. ACTA HYDROBIOLOGICA SINICA, 2025, 49(2): 022515. DOI: 10.7541/2024.2024.0053
    [2]GUO Xiao-Ye, LI Yan-Hua, HAN Dan-Xiang. ANALYSIS OF LIPIDOMES OF THE SYNECHOCYSTIS SP. PCC 6803 CELLS[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(2): 376-386. DOI: 10.7541/2021.2019.254
    [3]ZHU Lin, WANG Hou-Peng, ZHU Zuo-Yan, SUN Yong-Hua, YE Ding. MOLECULAR CLONING AND IDENTIFICATION OF NANOS3 IN BLUNT SNOUT BREAM (MEGALOBRAMA AMBLYCEPHALA)[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(3): 457-464. DOI: 10.7541/2019.056
    [4]SHI Jun, CHU Wu-Ying, ZHANG Jian-She. THE FUNCTIONAL STUDIES OF MUSCLE-SPECIFIC MICRORNAS[J]. ACTA HYDROBIOLOGICA SINICA, 2015, 39(6): 1224-1230. DOI: 10.7541/2015.159
    [5]Shahid Mahboob, Bilal Hussain, Zahid Iqbal, Abdul Shakoor Chaudhry. ESTIMATION OF VOLATILE CONSTITUENTS IN THE FISH FLESH FROM WILD AND FARMED CIRRHINA MRIGALA AND CYPRINUS CARPIO[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 484-491.
    [6]HUANG Wen-Min, XING Wei, LI Dun-Hai, LIU Yong-Ding. EFFECTS OF EXOGENOUS DMSO AND ASA ON FORMATION OF REACTIVE OXYGEN SPECIES AND ANTIOXIDANT SYSTEMS OF TOBACCO SUSPENSION CELLS UNDER MC-RR STRESS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(6): 869-870.
    [7]XIE Zhi-Gang, NIU Cui-Juan. EFFECTS OF PARTIAL AND COMPLETE FOOD DEPRIV ATION ON COMPENSATORY GROWTH OF JUVENILE SOFT -SHELLED TURTLE ( PELODISCUS SINENSIS):TEMPORAL PATTERNS IN GROWTH RA TE AND CHANGES IN BODY COMPOSITION[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 214-219.
    [8]CHEN Gang, ZHOU Hui, ZHANG Jian-Dong, WU Zao-He. HEMATOLOGICAL STUDY AND OBSERVATION ON DEVELOPMENT OF BLOOD CELLS IN COBIA, RACHYCENTRON CANADUM[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(5): 564-570.
    [9]CHENG Xiao-Li, CHENG Ying-Hui, LIU Hui, LU Wen-Qing. EFFECT OF CHLORINATED DRINKING WATER EXTRACTSON LIPID PEROXIDATION IN HEPG2 CELLS[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(1): 38-42.
    [10]Wu Jihua, Liang Yanling, Sun Xida. NEWLY RECORDED SPECIES OF FREE-LIVING NEMATODES FROM CHINA (CHROMADORIDA,ENOPLIDA ARAEOLAIMIDA)[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 320-321.

Catalog

    Get Citation
    PDF
    XML
    Article views (1028) PDF downloads (488) Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return