CHEN Chang fu, CHEN Chao ran. IMMUNOGENICITY OF CRUDE LIPOPOLYSACCHARIDE OF THREE PATHOGENIC BACTERIA OF FISH TO SILVER CRUCIAN CARP, CARESSIUS AURATUS GIBELIO[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 483-488.
Citation: CHEN Chang fu, CHEN Chao ran. IMMUNOGENICITY OF CRUDE LIPOPOLYSACCHARIDE OF THREE PATHOGENIC BACTERIA OF FISH TO SILVER CRUCIAN CARP, CARESSIUS AURATUS GIBELIO[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 483-488.

IMMUNOGENICITY OF CRUDE LIPOPOLYSACCHARIDE OF THREE PATHOGENIC BACTERIA OF FISH TO SILVER CRUCIAN CARP, CARESSIUS AURATUS GIBELIO

  • Received Date: October 19, 2001
  • Rev Recd Date: November 29, 2001
  • Published Date: September 24, 2002
  • The silver crucian carp)Caressius auratus gibelio)were immunized with crude lipopolysaccharide(LPS)extracted from Cytophaga columnaris、Aeromonas hydrophila and Vibrio anguillarum .The relative percent survival(RPS)of silver crucian carp were compared by examing the agglutinating antibody titers,cross agglutinating antibody titers of serum and the phagocytic activity of phagocytes in the blood and head kidney challenged with live A. hydrophila .The experimental results showed that the agglutinating antibody titers and cross agglutinating antibody titers of immunized silver crucian carp were higher than the control group,and the phagocytic activity of phagocytes in the blood and head kidney of silver crucian carp was significantly boosted and had a high level cross RPS.The crude LPS of three pathogenic bacteria were thought to have a common protective antigens to causing a cross RPS to silver crucian carp.
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    陈昌福,李静,吴志新.不同方法提取两种鱼类病原菌脂多糖的化学成分分析[J].华中农业大学学报,1996,15(5):481-483[2] 陈昌福,罗宇良,曾妍雄,等.不同血清型柱状嗜纤维菌脂多糖蛋白质成分及其对鳜的免疫原性影响的研究[J].华中农业大学学报,2000,19(5):483 -486[3] 吴志新,陈昌福.三种爱德华氏菌脂多糖对日本NFDD1NFDD2免疫原性的比较 [J].水生生物学报,1998,22(增刊):126-131[4] 陈昌福.柱状嗜纤维菌的血清型与其菌苗抗原性的关系[J].华中农业大学学报,1997,16(6):585-588[5] Pacha R E,Poter S.Characteristics of myxobacteria isolated from the surface of freshwater fish[J].Appl Microbiol,1968 ,16:1901-1906[6] 陈昌福,李静.翘嘴鳜细菌性败血症病原菌的分离及致病力的研究[J].华中农业大学学报,1996,15(4):119-121[7] 楠田理一,佐古浩,川合研儿.病鱼から分离されたVibrio属细菌の分类学的研究-Ⅰ.形态学的,生物学的,ならびに生化学的性状にょゐ检讨[J].鱼病研究,1979,13(3):123-137[8] 吉水守,木村乔久,板村捻.サケ科鱼类の肠内细菌丛に关する研究-Ⅰ .饲育鱼の肠内细菌と细菌丛[J].日本水产学会志,1976,42(1):91-99[9] Whistler R L,BeMiller J N,Wolfrom M L,et al.Methods in carbohydrate chemistry[M].New York:Academic Press,1965[10] Graham S,Jeffries A H,Secombes C J.A nevel assay to ditect macrophage ba ctericidal activity in fish :factous influencing the killing of Aeromonas salm onicida[J].J Fish Dis.,1988,11,389-396[11] Klein D.,Ono H.,OhUigin C,et al.Exten Sive MHC variability in cichlid fi shes of Lake Malawi[J]. Nature, 1993,364:330-334[12] 稻田善和,筑紫康博,迁村明夫,谷口顺彦.ビブリォ病に对するァュの免疫的形质の评价[J].水产育种,1996,23(1):29-39[13] 董仕,谷口顺彦.RAPD-PCRおよびァィザィムパタ-ンによるギンブナ一腹子のクロ-ン性の证明[J].日本水产学会志,1996.62(6):891-896

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