Volume 33 Issue 4
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CAO Hong, ZHOU Li, GUI Jian-Fang. EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR2OLD　CHINESE STURGEON PITUITARY[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(4): 581-588.
Citation: CAO Hong, ZHOU Li, GUI Jian-Fang. EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR2OLD　CHINESE STURGEON PITUITARY[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(4): 581-588.
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EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR2OLD　CHINESE STURGEON PITUITARY

  • State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan430072;
    2. Graduate School of the Chinese Academy of Sciences, Beijing100039

Funds: 

the National Major Basic Research Program (2004CB117401)

  • Received Date: June 25, 2008
  • Rev Recd Date: November 30, 2008
  • Published Date: July 24, 2009
    Graphical Abstract
    • Abstract
  • Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and for profiling geneexpression. In order to isolate specific functional genes involved in reproduction and endocrine regulation and to reveal their evolutionary mechanisms in Chinese sturgeon (Acipenser sinensis Gray), a chondrostean fish with a history of 140 million years, we constructed its pituitary cDNA library from a 4 year-old male1 A total of 944 random cloneswere sequenced and compared with sequences in GenBank database1 Among all the 944 EST clones, 802 (84196%) clones were identified as 461 known genes, and additional 142 (15104%) as unknown genes. Functional categorization indicated that the most abundantly expressed functional gene was the proopiomelanocortin (POMC), which accounted for almost10117% of the overall expression, indicating its important function in the pituitary. Interestingly, the expression patterns of 7 unknown geneswere analyzed in various tissues, such as heart, liver, spleen, kidney, muscle, testes, ovary and pituitary. Three different categories of expression patternswere observed from them1 Several unknown ESTs, such as EG009334, EG009337, EG009338 and EG009340, were detected to be pituitary-specific, orpituitary and ovary-specific genes. Further　studies on their functions will be very useful for better understanding the mechanisms of sturgeon reproduction　biology and endocrinology.
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    • References (36)
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