DETECTION OF SPRING VIREMIA OF CARP VIRUS)SVCV)GENE USING REVERSE-POLYMERASE CHAIN-REACTION)RT-PCR)

Spring viremia of carp virus(SVCV)is a rhabdovirus of fish that causing serious mortalities of carp. A rapid, sensitive and specific detection method was being set up for diagnosis of SVCV used RT PCR and semi nested PCR by the authors. The target regions of the SVCV glycoprotein gene were amplified using R2-F1 and R2-F1 primers. The products were 714bp segment in RT-PCR and 606bp segment in semi nested PCR, respectively. These primers could not amplified RNA of IHNV, VHSV and PFRV. The sensitivity has a detection limit of 1000 TCID50.