PANG Yan-bin, SUN Wei-hua, LI Shu-qiang. STUDIES ON CORTICAL CYTOSKELETAL PROTEINS OF DIFFERENT SPECIES OF TETRAHYMENA[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 241-244.
Citation: PANG Yan-bin, SUN Wei-hua, LI Shu-qiang. STUDIES ON CORTICAL CYTOSKELETAL PROTEINS OF DIFFERENT SPECIES OF TETRAHYMENA[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 241-244.

STUDIES ON CORTICAL CYTOSKELETAL PROTEINS OF DIFFERENT SPECIES OF TETRAHYMENA

  • Received Date: August 23, 1999
  • Rev Recd Date: September 29, 2000
  • Published Date: May 24, 2001
  • Cortical cytoskeletal proteins had been prepared by biochemical extraction with Tritonx-100, SDS-PAGE and scan, in order to study their difference of Tetrahymena thermophila BT, Tetrahymena thermophila BF and Tetrahymena shanghaiensis S1. The data statistics had been analyzed by a computer. The results showed that the difference between T. thermophila BT and T. thermophila BF was little. The T. shanghaiensis S1 had more protein patterns than those of two strains of T. thermophila. These proteins were 92KD, 72KD, 66KD, 32KD and 27KD. So the difference between Shanghaiensis S1 thermophila and the two strains of T. thermophila was greater. We think that it was because of the difference of cultive medium and conditions that affected the components and contents of proteins.
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    庞延斌等.镰游仆虫Euplotes harpa的皮层细胞骨架构造[J].华东师范大学学报(神经生物学与原生动物学专辑),1992,46—60[2] Vaudaux P E.Isolation and identification of specific cortical proteins in Tetrahymena pyriformis stain[J].J.Protozool,1976,23(3):458—464[3] Wolf J.Structural analysis of the isolated oral apparatus of Tetrahymena pyriformis[J].J.Cell Sci.,1970,6:679[4] Williams N E.et al.Oral filament proteins and their regulation in Tetrahymena pyriformis[J].J.Cell Res.,1986,164:295[5] Numata D.et al.Tetrahymena 14-nm filament forming protein has citrate synthase activity [J].Biochemical and Biophysical Research Communication,1991,174:1028[6] Howare E,Bushe J R.et al.Comparison of cortical protein in Tetrahymena vorax microstomes and macrostomes [J].J.Protozool,1982,29(2):222[7] Laemmli U K.Cleavage of structural proteins during the Assembly of the Head of Bacteriophage T4 [J].Nature,1970,227(15):680[8] 萨姆布鲁克等.分子克隆实验指南(第二版)[M].北京;科学技术出版社,1995[9] Elliot A M Biology of Tetrahymena[M].New York: Appleton,century crofts,Inc.,1971[10] Aaronson S,Baker H.Lipid and Sterol content of some Protozoa [J].J.Protozool,1961,8:274

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