Zhu Bin, Chang Jianbo, Tan Xichang, Yu Gongliang, Xiao Congxue, Wu Zhiqiang. APPLICABILITY OF MICOSATELLITE DNA PRIMERS OF LAKE STURGEON FOR THE PARENTAGE ANALYZING OF CHINESE STURGEON[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(6): 547-553.
Citation:
Zhu Bin, Chang Jianbo, Tan Xichang, Yu Gongliang, Xiao Congxue, Wu Zhiqiang. APPLICABILITY OF MICOSATELLITE DNA PRIMERS OF LAKE STURGEON FOR THE PARENTAGE ANALYZING OF CHINESE STURGEON[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(6): 547-553.
Zhu Bin, Chang Jianbo, Tan Xichang, Yu Gongliang, Xiao Congxue, Wu Zhiqiang. APPLICABILITY OF MICOSATELLITE DNA PRIMERS OF LAKE STURGEON FOR THE PARENTAGE ANALYZING OF CHINESE STURGEON[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(6): 547-553.
Citation:
Zhu Bin, Chang Jianbo, Tan Xichang, Yu Gongliang, Xiao Congxue, Wu Zhiqiang. APPLICABILITY OF MICOSATELLITE DNA PRIMERS OF LAKE STURGEON FOR THE PARENTAGE ANALYZING OF CHINESE STURGEON[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(6): 547-553.
Institute of hydrobiology, The Chinese Academy of Sciences; 2. State Key Laboratsry of Freshwater Ecology and Biotechnology, Wuhan 430072; 3. Department of Zoology, University of Toronto, Toronto, Ontario M4Y 1R5, Canada
Four miscrosatellite loci of lake sturgeon (Acipenser fulvescens) were amplified from the selected individuals of Chinese sturgeon ( A .sinensis) in random.All loci were amplified well and homologous sequences were obtained from all samples.One locus was monomorphic, and the others polymorphic.Among them, two loci were detected having over three alleles.Each individual of matured Chinese sturgeon captured in 1999 could be distinguished with the fingerprinting maps of the two microsatellite loci.This indicates that the two polymorphic microsatellite loci could be used for analyzing the parentage relationships of Chinese sturgeon, and thus differentiating individuals of the artificial released and the natural propagated ones.
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