USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR

Ouyang Bin; Chang Jianbo

Volume 23 Issue 4

Feb. 2014

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ACTA HYDROBIOLOGICA SINICA > 1999 > 23(4): 324-329.

Ouyang Bin, Chang Jianbo. USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(4): 324-329.

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Ouyang Bin, Chang Jianbo. USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(4): 324-329.

Citation:

Ouyang Bin, Chang Jianbo. USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(4): 324-329.

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USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR

Insitute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072

Received Date: August 06, 1998
Rev Recd Date: June 16, 1999
Published Date: July 24, 1999

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Abstract

The present paper deals with methods and effects of using alizarincomplexone (ALC)and chlorydrate of tetracycline (CHTC) to mark larval and juvenile Gobiocypris rarus and Carassius auratus pengze Var. To immerse the two species in 50-100mg/ L ALC solutions for 6-24h, a well-defined mark on otolith was induced.A well-defined mark on otolith was also observed when immersing larval Carassiusauratus pengze Var in 150-250mg/L CHTC solutions for 12-24h. However, onlyimmersing -caused mortality was observed in G. rarus when immersed in CHTC solution. suggesting that TC may not be suitable for being used as marking solution.Under UV light,the mark of ALC in the otoliths is a scarlet-pink fluorescent zone,and that of CHTC is a yellow fluorescent zone.
- Alizarin complexone,
- Chlorhydrate of tetracycline,
- Mark,
- Larvae andjuvenile fish

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[1]	Hettler W F. Marking otoliths by immersion of marine fish larvae in tetracycline. Transactions of the American Fisheries Society, 1984, 113:370-373
[2]	Schmitt P D. Marking growth increments in otoliths of larval and juvenile fish by immersion in tetracycline to examine the rate of increment formation. U. S. National Marine Fisheries Service Fishery Bulletin, 1984, 82:237-241
[3]	Tsukamoto K. Mass-marking of ayu eggs and larvae by tetracycline-tagging of otoliths. Bulletin of the japanese Society of Scientific Fisheries, 1985, 51: 903-911
[4]	Wilson C A. Beckman D W, Dean J M. Calcein as a fluorescent marker of larval and juvenile fish.Transactions of the American Fisheries Society, 1987, 116:668-670
[5]	Tsukamoto K. Otolith tagging of ayu embryo with fluorescent substances. Nippon Suisan Gakkaishi, 1988,54:1289-1295
[6]	董双林、王志余、于信勇等.鲤鱼仔、稚和幼鱼早期耳右上日轮的初步观察.大连水产学院学报,1989,4(1):58-62
[7]	Beckman D W, Schulz R G. A simple method for marking fish otoliths with alizarin compounds.Transactions of the American Fisheries Society, 1996, 125, 146-149
[8]	Vigliola L. Validation of daily increment formation in otoliths for three Diplodus species in the Mediterranean sea. Journal of Fish Biology, 1997, 51, 349-360

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USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR

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