Volume 28 Issue 1
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LIU Xiao-YunZHANG, Zhi-Feng, LIAO Cheng-Yi. STUDIES ON ULTRASTRUCTURE AND CYTOCHEMISTRY OF MIDGUT INPENAEUS CHINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 58-62.
Citation: LIU Xiao-YunZHANG, Zhi-Feng, LIAO Cheng-Yi. STUDIES ON ULTRASTRUCTURE AND CYTOCHEMISTRY OF MIDGUT INPENAEUS CHINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 58-62.
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STUDIES ON ULTRASTRUCTURE AND CYTOCHEMISTRY OF MIDGUT INPENAEUS CHINENSIS

  • Test Center of Ocean University of Zhongguo;
    2. College of Marine Life Sciences, Qingdao, 266003

  • Received Date: April 10, 2003
  • Rev Recd Date: September 09, 2003
  • Published Date: January 24, 2004
    Graphical Abstract
    • Abstract
  • The electronic microscope technique and the enzyme cyt ochemistry technique were used to studied the ultrastructure of the wall of the midgut of penaeus chinensis, and active site of phenoloxidasa (PO) and acid phosphatase (ACP) in the tissue cells of midgut well. Right midgut from penaeus chinensis were removed and making experiments using three methods: ①midgut tissue was cut into blocks of approximately 1mm 3, the blocks were fixed in 3% fresh glutaraldehyde solution and then were post-fixed in 1% osmic acid. Embeldded it in Epon821. Semit him sections of the midgut ware cut on the ULTRACUT E. Stained it with toluidine blue and examined under microscope. Ultrathin sections of the midgut were cut on the LKB-Nova ultramicrotome. ②The midgut tissue blocks were fixed in 2% glut araldehyde solutions, then the blocks cut into thin sections approximately 60nm and incubated in the ACP medium and post-fixed in 1% osmic acid, embedded it in Epon821 and cut it into ultrathin sections. ③the midgut tissue blocks of healt hy and virus-infectious penaeus chinensis were fixed in 1% Bakers formol calciu m-and cut it into thin sections approximately 100 nm, incubated it in the pheno l oxidase medius then deal the samples with common ultrasection technique. All abo ve ultrathin sections were observed under a Hitachi H-7000 electron microscope. The results showed: the epithelium of midgut includes light cells and da rk cells, mostly dark cells, both kinds of cells have the function of absorbing nutrient substances from the enterocolic cavity and transferring them to connect ive tissue. Cytochemistry reaction results showed: ACP positive reaction was sho wn in the rounded vesicles that are situated at the top of the light cells, also in the lysosomal bodies of both light and dark cells. The tops of the dark cell s were crowded with lots of ACP-negative zymogen granules that were mainly synt hesized and excreted by the dark cells. PO hardly have activity in health y wall of the midgut, while in the virus-infections penaeus chinensis, the PO- granules were found on the disassembled granular cells of the connective tissue and dissociate in the connective tissue. Besides maintaining the shapes of the epithelial cells and stoking nutriments, the connective itssue play role in the i mmune reaction of the penaeus chinensis.
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  • [1]
    Rigdon R H, Mensik D J. Gastrointestinnal tract of penaeus aztecusives, 1891(Decapoea, Natantia), Aistologicstudy [J]. Grustaceana, 1976, 30(2): 164-168[2] Thomas A B, Donald VL. A Handbook of normal penaseid shrimp histology [M]. Prinled in the united states of Amenrica by Allen Press. Inc. Lawrence, Kansas 1988, 86-89[3] Gao J, yang Z, Ma S. A preliminary study of the development of the digestive system of Penaeus orientalis kishinouye [J]. Journal of shandong college of oceanology, 1986, 16(4): 18-23.[高杰,杨泽,马NB059.中国对虾(Penaeus chinensis)消化系统发生的初步研究. 山东海洋学院学报,1986,16(4):18-23][4] Wang h L, Liao C Y, Zang Z F. Study on the genesis of the digestive system of prawn Penaeus chinensis[J]. Journal of ocean university of qingdao, 1997, 27(3): 339-346.[王海林,廖承义,张志峰. 中国对虾(Penaeus chinensis)消化系统发生的研究. 青岛海洋大学学报,1997,27(3):339-346][5] Zhang Z F, Ma A J, Yu L. Ultrastructure of the midgut in the larvae of Penaeus chinensis [J]. Oceanologia et Limnologia Sinica, 1999, 30(2): 145-150.[张志峰,马爱军,于利. 中国对虾幼体中肠的超微结构. 海洋与湖沼,1999,30(2):145-150][6] Luo R X, Jang Y X, Li G Y. Studies on the phenoloxidase activities in homocytes of the shrimp [J]. Experiments and Technigues, 1996, 6:1-3.[罗日祥,姜玉香,李光友. 中国对虾血细胞中酚氧化酶活力研究. 实验与技术,1996,6:1-3][7] Li T D, Yu J, Yu K K. Studies on the phenoloxidese activities in the serum of Penaeus chinensis [J]. Transactions of Oceanology and limnology, 1998, 1: 51-56.[李天道,于佳,俞开康. 中国对虾血清中酚氧化酶活力研究. 海洋湖沼通报,1998,1:51-56][8] Zhong C S. Enzyme histochemistry and cytochemistry Shanghai Medical university of Shanghai Press, 1987, 111-112.[钟慈声,酶细胞化学技术. 上海:上海医科大学出版社,1987,111-112][9] Coles J A, Pipe R K. Phenoloxidase activity in the haemolymph and haemocytes of the marine massel mytilus edulis[J]. Fish shellfish immuol. 1994,4:337-352[10] Liu X Y, Sheng X Z, Jiang M. Studies of midgut cecum of penaeus chinensis [J]. Marine Fisheries Research, 1998, 19(1):1-7.[刘晓云,绳绣珍,姜明. 中国对虾中肠盲囊的研究. 海洋水产研究,1998,19(1):1-7][11] Soderhall K. β 1,3-Glucan enhancement of Protease activity in crayflish hemocyte lysate [J]. Comp. Biochem. Physiol., 1983, 74B:221-224[12] Soderhall K. Separation of the hemocyte populations of Carcinus maenas and other marine decapods [J]. Dev. Comp. Immune., 1983, 7:229-239[13] Soderhall K. The properties and purification of a Blaberus craniifer plasma protein which enhances the activation of haemocyte prophenoloxidase by a β 1,3-Glucan [J]. Insect Biochem., 1988, 18: 323-330[14] Meng F L, Zhang Y Z, Kong J, et al. The research review of prophenoloxidase activating system in crustacean. Ocenologia et Limnologia Sinica, 1999, 30(1): 110-115.[孟凡伦,张玉臻,孔健,等. 甲壳动物中的酚氧化酶原激活系统研究评价. 海洋与湖沼,1999,30(1):110-115][15] Li G R, Zhang S C, Li H Y, et al. Advance on the study of phenoloxidase Ⅰ [J]. Marine Sciences, 2003, 27(4):4-8.[李国荣,张士璀,李红岩,等. 酚氧化酶研究概况Ⅰ. 海洋科学,2003,27(4):4-8][16] Li G Y. Review of disease and immune mechanism in penaeus chinensis[J].Marine Sciences, 1995, 4:1-3.[李光友. 中国对虾疾病与免疫机制. 海洋科学,1995,4:1-3][17] Liu X Y, zhang Z F,Ma H m. Study on enzyme cyto-chemistry of blood cell in penaeus chinensis[J]. Journaal of ocean university of Qingdao, 2002, 32(2): 259-265.[刘晓云,张志峰,马洪明. 中国对虾血细胞酶细胞化学的初步研究. 青岛海洋大学学报,2002,32(2):259-265][18] Guan H S. Immunology, cell cultivation and disease studies of marine cultural animals[M]. Jinan: Shandon Science and Technology Press, 1999: 1-31. [管华诗主编. 海水养殖动物的免疫、细胞培养和病理研究. 济南: 山东科学技术出版社,1999,1-31][19] Cai S L, Wang C M, Yang C H. Studies of pathogeny of white and black spot disease on penaeid shrimp[J]. Journal of Fisheries of China, 1996, 20(4):325-331.[蔡生力,王崇明,杨丛海, 等. 对虾白黑斑的病原病因研究. 水产学报,1996,20(4):325-331][20] Wang L, Li G Y. Advance in the studies of humoral immunity in crustaceans [J]. Marine Sciences, 1992, 3:18-19.[王雷,李光友. 甲壳动物的体液免疫研究进展. 海洋科学,1992,3:18-19][21] Liu X Y, Zhang Z F, Yu L et al. Studys on the phenoloxidase activites in the tissue cell of Fenneropenaus Chinensis[J]. 2002,12(140):89-92.[刘晓云, 张志峰, 于力, 等. 中国对虾组织细胞中酚氧化酶的研究. 高技术通讯,2002,12(140):89-92][22] Liu X Y, Zhang Z F Jiang M et al. The phenoloxidase in lymphatic organ of Penaeus Chinensis [J]. Journal of Fisheries of Chinensis[J], 2002,26(3):285-288.[刘晓云, 张志峰, 姜明,等. 中国对虾淋巴器的酚氧化酶. 水产学报,2002,26(3):285-288]

    Rigdon R H, Mensik D J. Gastrointestinnal tract of penaeus aztecusives, 1891(Decapoea, Natantia), Aistologicstudy [J]. Grustaceana, 1976, 30(2): 164-168[2] Thomas A B, Donald VL. A Handbook of normal penaseid shrimp histology [M]. Prinled in the united states of Amenrica by Allen Press. Inc. Lawrence, Kansas 1988, 86-89[3] Gao J, yang Z, Ma S. A preliminary study of the development of the digestive system of Penaeus orientalis kishinouye [J]. Journal of shandong college of oceanology, 1986, 16(4): 18-23.[高杰,杨泽,马NB059.中国对虾(Penaeus chinensis)消化系统发生的初步研究. 山东海洋学院学报,1986,16(4):18-23][4] Wang h L, Liao C Y, Zang Z F. Study on the genesis of the digestive system of prawn Penaeus chinensis[J]. Journal of ocean university of qingdao, 1997, 27(3): 339-346.[王海林,廖承义,张志峰. 中国对虾(Penaeus chinensis)消化系统发生的研究. 青岛海洋大学学报,1997,27(3):339-346][5] Zhang Z F, Ma A J, Yu L. Ultrastructure of the midgut in the larvae of Penaeus chinensis [J]. Oceanologia et Limnologia Sinica, 1999, 30(2): 145-150.[张志峰,马爱军,于利. 中国对虾幼体中肠的超微结构. 海洋与湖沼,1999,30(2):145-150][6] Luo R X, Jang Y X, Li G Y. Studies on the phenoloxidase activities in homocytes of the shrimp [J]. Experiments and Technigues, 1996, 6:1-3.[罗日祥,姜玉香,李光友. 中国对虾血细胞中酚氧化酶活力研究. 实验与技术,1996,6:1-3][7] Li T D, Yu J, Yu K K. Studies on the phenoloxidese activities in the serum of Penaeus chinensis [J]. Transactions of Oceanology and limnology, 1998, 1: 51-56.[李天道,于佳,俞开康. 中国对虾血清中酚氧化酶活力研究. 海洋湖沼通报,1998,1:51-56][8] Zhong C S. Enzyme histochemistry and cytochemistry Shanghai Medical university of Shanghai Press, 1987, 111-112.[钟慈声,酶细胞化学技术. 上海:上海医科大学出版社,1987,111-112][9] Coles J A, Pipe R K. Phenoloxidase activity in the haemolymph and haemocytes of the marine massel mytilus edulis[J]. Fish shellfish immuol. 1994,4:337-352[10] Liu X Y, Sheng X Z, Jiang M. Studies of midgut cecum of penaeus chinensis [J]. Marine Fisheries Research, 1998, 19(1):1-7.[刘晓云,绳绣珍,姜明. 中国对虾中肠盲囊的研究. 海洋水产研究,1998,19(1):1-7][11] Soderhall K. β 1,3-Glucan enhancement of Protease activity in crayflish hemocyte lysate [J]. Comp. Biochem. Physiol., 1983, 74B:221-224[12] Soderhall K. Separation of the hemocyte populations of Carcinus maenas and other marine decapods [J]. Dev. Comp. Immune., 1983, 7:229-239[13] Soderhall K. The properties and purification of a Blaberus craniifer plasma protein which enhances the activation of haemocyte prophenoloxidase by a β 1,3-Glucan [J]. Insect Biochem., 1988, 18: 323-330[14] Meng F L, Zhang Y Z, Kong J, et al. The research review of prophenoloxidase activating system in crustacean. Ocenologia et Limnologia Sinica, 1999, 30(1): 110-115.[孟凡伦,张玉臻,孔健,等. 甲壳动物中的酚氧化酶原激活系统研究评价. 海洋与湖沼,1999,30(1):110-115][15] Li G R, Zhang S C, Li H Y, et al. Advance on the study of phenoloxidase Ⅰ [J]. Marine Sciences, 2003, 27(4):4-8.[李国荣,张士璀,李红岩,等. 酚氧化酶研究概况Ⅰ. 海洋科学,2003,27(4):4-8][16] Li G Y. Review of disease and immune mechanism in penaeus chinensis[J].Marine Sciences, 1995, 4:1-3.[李光友. 中国对虾疾病与免疫机制. 海洋科学,1995,4:1-3][17] Liu X Y, zhang Z F,Ma H m. Study on enzyme cyto-chemistry of blood cell in penaeus chinensis[J]. Journaal of ocean university of Qingdao, 2002, 32(2): 259-265.[刘晓云,张志峰,马洪明. 中国对虾血细胞酶细胞化学的初步研究. 青岛海洋大学学报,2002,32(2):259-265][18] Guan H S. Immunology, cell cultivation and disease studies of marine cultural animals[M]. Jinan: Shandon Science and Technology Press, 1999: 1-31. [管华诗主编. 海水养殖动物的免疫、细胞培养和病理研究. 济南: 山东科学技术出版社,1999,1-31][19] Cai S L, Wang C M, Yang C H. Studies of pathogeny of white and black spot disease on penaeid shrimp[J]. Journal of Fisheries of China, 1996, 20(4):325-331.[蔡生力,王崇明,杨丛海, 等. 对虾白黑斑的病原病因研究. 水产学报,1996,20(4):325-331][20] Wang L, Li G Y. Advance in the studies of humoral immunity in crustaceans [J]. Marine Sciences, 1992, 3:18-19.[王雷,李光友. 甲壳动物的体液免疫研究进展. 海洋科学,1992,3:18-19][21] Liu X Y, Zhang Z F, Yu L et al. Studys on the phenoloxidase activites in the tissue cell of Fenneropenaus Chinensis[J]. 2002,12(140):89-92.[刘晓云, 张志峰, 于力, 等. 中国对虾组织细胞中酚氧化酶的研究. 高技术通讯,2002,12(140):89-92][22] Liu X Y, Zhang Z F Jiang M et al. The phenoloxidase in lymphatic organ of Penaeus Chinensis [J]. Journal of Fisheries of Chinensis[J], 2002,26(3):285-288.[刘晓云, 张志峰, 姜明,等. 中国对虾淋巴器的酚氧化酶. 水产学报,2002,26(3):285-288]
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