Volume 29 Issue 1
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
LIU Yan-Ming, ZHANG Qi-Ya, YUAN Xiu-Ping. STUDIES ON ABUNDANCE AND MORPHOLOGICAL DIVERSITY OF VIRIOPLANKTON IN THE DONGHULAKE, WUHAN[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(1): 1-6.
Citation: LIU Yan-Ming, ZHANG Qi-Ya, YUAN Xiu-Ping. STUDIES ON ABUNDANCE AND MORPHOLOGICAL DIVERSITY OF VIRIOPLANKTON IN THE DONGHULAKE, WUHAN[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(1): 1-6.
shu

STUDIES ON ABUNDANCE AND MORPHOLOGICAL DIVERSITY OF VIRIOPLANKTON IN THE DONGHULAKE, WUHAN

  • State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072 Graduate school of the Chinese Academy of Sciences, Beijing 100039

  • Received Date: May 17, 2004
  • Rev Recd Date: June 10, 2004
  • Published Date: January 24, 2005
    Graphical Abstract
    • Abstract
  • Virioplankton,viruses in aquatic ecosystem,are now considered to be an abundant and dynamic component in the aquatic microbial communities that can regulate the biomass production and species composition of heterophic bacteria,cyanobacteria,and phytoplankton,influence biogeochemical cycling,and mediate gene transfer between microorganisms in aquatic ecosystems.Lake Donghu located in Wuhan city was a eutrophic shallow lake,which contains three trophic regions:hypereutrophic,eutrophic and mesophic region. To assess the ecological role of virioplankton in Lake Donghu,it is necessary to determine the abundance and the morphotype diversity of virioplankton. The virioplankton abundance was measured by transmission electron microscopy (TEM) direct counts and epifluorescence microscopy (EM) direct counts using SY BRG old stain in Lake Donghu. All data were analyzed by SAS811 (SATA) software packages (SAS Institute Inc.). Water column virioplankton abundances were revealed highest numbers (9.74 × 108mL-1) in eutrophic region and lowest numbers(7.68 × 108mL-1) in mesotrophic region by TEM.Virioplankton direct counts of hypereutrophic and eutrophic regions were significantly higher (P <0.05) than those of mesotrophic region.Virioplankton abundances counted by EMwere significantly higher than those counted by TEM (P <0.01),up to 2.72 times on average. The analysis of morphological diversity of virioplankton by TEM showed that most virioplankters observed were similar to bacteriophages and cyanophages which had diverse tails and hexagonal heads. According to morphotype,most of them belong to Siphoviridae (Tail is noncontratile,long),Myoviridae (Tail is contractile,long and complex,consisting of a central tube and a contratile sheath separated from the head by a neck) and Podoviridae (Tail is short about 20 nm and noncontratile). In addition,filovirus,baculovirus,bullet2shaped virus,rod-shaped virus, and other viruses,were also detected. Some virus-like particles observed with clear capsomer were similar to human and animal virus. In water samples,virioplankton abundance detected was much more than those of bacterioplankton,cyanobacteria and algae. The results suggest that virioplankton played vital roles in water environment and ecosystem of Lake Donghu.
    • FullText(HTML)
    • References (23)
  • [1]
    Shao L G,You YJ,Zeng M X,et al.The nutrients-enriched water inthe East Lake of Wuhan and phosphorus-combating strategies[J].J.of Wuhan Yejin Uni.of Sci.& Tech,1999,22(2):139-141[劭林广,游映玫,曾明秀,等.武汉东湖水体富营养化现状及其控磷 对策.武汉冶金科技大学学报,1999,22(2):139-141]
    [2]
    G ong ZJ,Xie P.Impact of eutrophicationon biodiversityof the macrozoobenthos community in a Chinese shallow lake [J].J.Freshwater Ecology,2001,16(2):171-177
    [3]
    Liu D S,Luo Q F.Distribution and role of dentrifying,nitrifying,nitrosetion and ammonifying bacteria in East Lake [J].Environmental Science,2002,23(3):29-35[刘东山,罗启芳.东湖氮循环细菌分布及其作用[J].环境科学,2002,23(3):29-35]
    [4]
    Lei A P,Shi ZX,Wei Y X, et al.Diversity of the phytoplankton in Donghu Lake,Wuhan[J].Acta Hydrobiologia Sinica,2003,27 (2):179-184[雷安平,施之新,魏印心等.武汉东湖浮游藻类物种多样性的研究.水生生物学报,2003,27(2):179-184]
    [5]
    Wommack K E,Colwell R R.Virioplankton:viruses in aquatic ecosystems[J].Microbiol.Mol.Biol.Rev,2000,64:69-1144水生生物学报 29 卷
    [6]
    Bergh O,Borsheim K Y,Bratbak G, et al.High abundance of viruses found in aquatic environments[J].Nature,1989,340:467-468
    [7]
    Fuhrman J A.Marine viruses and their biogeochemical and ecological effects[J].Nature,1999,399:541- 547
    [8]
    Proctor L M, Fuhrman J A.Viral mortality of marine bacteria and cyanobacteria[J].Nature,1990,343:60-62
    [9]
    Suttle C A,Chan A M,Cottrell M T.Infection of phytoplankton byviruses and reduction of primary productivity[J].Nature,1990,347:467-469
    [10]
    Thingstad T F,Heldal M,Bratbak G,et al.Are viruses important partners in pelagic food webs[J].Trends Ecol.E,1993,8:209-212
    [11]
    Wilhelm S W,Suttle CA.Viruses and nutrient cycles in the sea:viruses play critical roles in the structure andfunction of aquatic food webs[J].Bioscience,1999,49:781-788
    [12]
    Zhang Q Y.Virioplankton[J].Acta Hydrobiologia Sinica,2002,26(6):691-696[张奇亚.浮游病毒.水生生物学报,2002,26(6):691-696]<>br[13] Zhang C Y.The efficiency of two2step concentration method for recovery of viruses from water[J].China Environmental Science,1989,9(6):463-465[张楚瑜.两步浓缩法回收水中病毒的有效性.中国环境科学,1989,9(6):463-465]
    [14]
    Tang X C,Song L R,Sheng Y W, et al.Study of phycophages from Microcystis· spin Lake Dianchi,south2west China[J].Journal of Microbiology,2003,23(3):50-55[汤显春,宋立荣,沈银武,等.滇池微囊藻病毒溶藻的研究.微生物学杂志,2003,23(3):50-55]<>br[15] Brsheim K Y,Bratbak G,Heldal M.Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy[J].Appl Environ Microbiol,1990,56:352 -356
    [16]
    Chen F,LuJ R,Binder BJ,et al.Applicationof digital image analysisand flow cytometry to enumerate marine viruses stained with SY BRG old[J].Appl Environ Microbiol,2001,67:539-545
    [17]
    Jiang S,Steward G,Jellison R, et al.Abundance,Distribution,and Diversity of Viruses in Alkaline,Hypersaline Mono Lake,California[J].Microb Ecol,2004,47(1):9 -17
    [18]
    Cochla W P,WiknerJ,Steward GF, et al.Spatial distributionof viruses,bacteria and chlorophyll2a in neritic,Oceanic and estuarine environments[J].Mar Ecol Prog Ser,1993,92:77-87
    [19]
    Hara S,Terauchi K,K oike I.Abundance of viruses in marine waters:assessment by epifluorescence and transmission electron microscopy[J].Appl Environ Microbiol,1991,57:2731-2734
    [20]
    Wommack K E,Hill R T,Kessel M,et al.Ditribution of viruses in theChesapeake Bay[J].Appl Environ Microbiol,1992,58:2965-2970
    [21]
    Hara S, K oike I,Terauchi K, et al.Abundance of viruses in deepoceanic waters[J].Mar Ecol Prog Ser,1996,145:269-277
    [22]
    Maranger R,Bird D F,Juniper S K.Viral and bacterial dynamics inAretic sea ice during the spring algal bloom near Resolute[J], N.W.T., Canada.Mar Ecol Prog Ser,1994,111:121-127
    [23]
    Thingstad T F,Lignell R.Theoretical modelsfor the control of bacterialgrowth rate,abundance,diversity and carbon demand[J].Aquat Microb Ecol,1997,13:19-27
    [24]
    Gu ZL.Bioremediation of eutrophied lakes in China[J].Rural EcoEnvironment,2002,18(1):42-45[顾宗濂.中国富营养化湖泊的生物修复.农村生态研究,2002,18(1):42-45]
    [25]
    Liang S,Qian HL,Qi YZ.Problemon the red tide in Coastal China.Sea[J].Ecologic Science,2000,19 (4):44-50[梁松,钱宏林,齐雨藻.中国沿海的赤潮问题[J].生态科学,2000,19(4):44-50]
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views (967) PDF downloads (650) Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return