| Citation: |
FENG Shao-Long, KONG Zhi-Ming, WANG Wu-Xiang, WANG Xin-Ming, PENG Ping-An. GENOTOXICOLOGICAL STUDIES OF TWO PESTICIDES TO TADPOLES AND FROGS OF RANA NIGRINACULATA HALLOWEII BY MICRONUCLEI TEST AND SINGLE CELL GEL ELECTROPHORESIS ASSAY[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 52-57.
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Wang R F, Jin Z Y, Kong X S, et al. The background content of inorganic chemicals and mutagenicity assessment in vivo of the water from fuxian lake[J].Zoological Research,1999,20(2):99-103[王蕊芳,金志玉,孔祥生,等.抚仙湖无机污染物化学背景值及动物体内致突变性评定.动物学研究,1999,20(2):99-103][2] Chen, J J and Xia, Y Z. The micronucleus test of tadpoles Rana nigronaculata Hallowell,A system for detection of mutagens in fresh water [J].Acta Hydrobiologica Sinica, 1993,17(4):298-307[陈军建,夏宜铮,青蛙蝌蚪微核试验--一种水体诱变剂检测系统的建立.水生生物学报,1993,17(4):298-307][3] Geng D G, Zhang D S, Cheng W, et al. Effects of four herbicides on micronuclei and nuclear anomalies in tadpole erythrocytes of Bufo bufo gargarizans[J].Chinese Journal of Zoology,2000,35(1):12-16 .[耿德贵,张大生.四种除草剂对中华大蟾蜍蝌蚪红细胞微核及核异常的影响. 动物学杂志,2000,35(1):12-16][4] EPA of China, The criterion of environmental monitoring techniques, part IV-biomonitoring (aquatic environment)[M].Beijing: The press of environmental sciences of China,1986.[中国环保局,环境监测技术规范,第四册-生物监测(水环境部分).北京:中国环境科学出版社,1986][5] Ralph S, Petras M. Caged amphibian tadpoles and in situ genotoxicity monitoring of aquatic environments with the alkaline single cell gel electrophoresis (comet) assay[J].Mut. Res.,1998,413:235-250[6] Croft B A, Brown AWA. Response of arthropod natural enemies to insecticides[J].A. Rev. Entomol.,1975,20:285-336[7] DeBach P, Rosen D. Biological control by natural enemies [M].London:Cambridge University Press,1991,271-273[8] Chen W L, He J L. A quick and sensitive method of detecting DNA damage-comet assay[J].The Foreign Medicine:the Hygiene.1998,25(2):101-104[陈玮琳,何继亮.快速敏感检测DNA断裂的方法-彗星试验.国外医学卫生学分册,1998,25(2):101-104][9] He J, Liu Y Q. The advancement and application of single cell gel electrophoresis assay [J].The Foreign Medicine: the Hygiene,1997,24(2):85-89[何惧,刘玉清.单细胞凝胶电泳技术的研究进展与应用.国外医学卫生学分册,1997,24(2):85-89][10] Verschaeve L, Gilles J. Single Cell Gel Electrophresis assay in the earthworm for the detection of genotoxic compounds in Soils [J].Bull. Environ. Contam. Toxicol.,1995,54:112-119[11] Wang M S, Scheemzer P. A introduce on the single cell gel electrophoresis assay [J].Carcinogenesis Teratogenesis and Mutagenesis,1996, 8(2):112[王民生,Schmezer, P.碱性单细胞微量凝胶电泳技术简介.癌变*畸变*突变,1996,8(2):112][12] Mitchelmore C L,Chipman, J K. DNA strand breakage in aquatic organisms and potential value of the comet assay in environmental monitoring [J].Mut. Res.,1998,399:135-147[13] Goethem F V, Lison D, Kirsch-Volders M. Comparative evaluation of in vitro micronucleus test and the alkaline single cell gel electrophoresis assay for the detection of DNA damaging agents: gents: genotoxic effects of cobalt powder, tungsten carbide and cobalt-tungsten carbide[J].Mut. Res.,1997,392:31-43[14] Collins A R, Ai-guo, M, Duthie S J. The kinetcs of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells [J].Mut. Res.,1995,336:69-77[15] Singh N P, Stephens R E. Microgel electrophoresis: Sensitivity, mechanisms, and DNA electrostretching [J].Mut. Res.,1997,383:167-175[16] Abd-Allah G A, El-Fayoumi R I, Smith M J, et al. A comparative evaluation of aflatoxin B1 genotoxicity in fish models using the Comet assay [J].Mut. Res.,1998,446:181-188[17] Feng S L, Lou Y, Zhong Y, et al. Single cell gel electrophoresis assay in the earthworm for measuring the genotoxicity of pesticides to DNA [J].Journal of Nanjing University(Natural Sciences),2000,36(5):649-652[封少龙,罗屿,钟远,等.应用单细胞凝胶电泳技术测定农药对蚯蚓的DNA损伤.南京大学学报(自然科学),2000,36(5):649-652][18] Li G, Chen X L, Chen D. et al. The application and studies of micronucleus assays with fish to screen mutagen in water [J].Acta Hydrobiologica Sinica.2002,26(2):74-81[李谷,程晓莉,陈丹,等.鱼微核试验筛检水体诱变物的应用与研究. 水生生物学报,2002,26(2):74-81][19] Li B. The advancement on the research and development of insecticide industry [J].Pesticides,2000,39(4):6-9[李斌.杀虫剂研发进展.农药,2000,39)4):6-9][20] Xu X L, Han L J, Wang X P, et al. Control Effect of Imidacloprid 20 SL in field to three aphides [J].Pestcides,2000,39(5):26-29[许小龙,韩丽娟,王小平,等.吡虫啉20SL新剂型对三种蚜虫的田间防治效果.农药,2000,39(5):26-29][21] Xuan R C, Wang Q Q, Zheng W, et al. Study on the adsorption of imidacloprid in soils and the interaction mechanism [J].Acta Scientiae Circumstantiae,2000,20(2):198-201[宣日成,王琪全,郑巍,等.吡虫啉在土壤中的吸附及作用机理研究.环境科学学报,2000,20(2):198-201][22] Jiang X Y, Wang K Y,Yi M J, et al. Studies on the Toxicity of Four species aphid and selectivity of natural enemy to synergistic imidacloprid[J].Pesticides,2000,39(9):26-27[姜兴印,王开运,仪美芹,等.增效吡虫啉对4种蚜虫的毒力和对天敌的选择性.农药,2000,39(9):26-27]
Wang R F, Jin Z Y, Kong X S, et al. The background content of inorganic chemicals and mutagenicity assessment in vivo of the water from fuxian lake[J].Zoological Research,1999,20(2):99-103[王蕊芳,金志玉,孔祥生,等.抚仙湖无机污染物化学背景值及动物体内致突变性评定.动物学研究,1999,20(2):99-103][2] Chen, J J and Xia, Y Z. The micronucleus test of tadpoles Rana nigronaculata Hallowell,A system for detection of mutagens in fresh water [J].Acta Hydrobiologica Sinica, 1993,17(4):298-307[陈军建,夏宜铮,青蛙蝌蚪微核试验--一种水体诱变剂检测系统的建立.水生生物学报,1993,17(4):298-307][3] Geng D G, Zhang D S, Cheng W, et al. Effects of four herbicides on micronuclei and nuclear anomalies in tadpole erythrocytes of Bufo bufo gargarizans[J].Chinese Journal of Zoology,2000,35(1):12-16 .[耿德贵,张大生.四种除草剂对中华大蟾蜍蝌蚪红细胞微核及核异常的影响. 动物学杂志,2000,35(1):12-16][4] EPA of China, The criterion of environmental monitoring techniques, part IV-biomonitoring (aquatic environment)[M].Beijing: The press of environmental sciences of China,1986.[中国环保局,环境监测技术规范,第四册-生物监测(水环境部分).北京:中国环境科学出版社,1986][5] Ralph S, Petras M. Caged amphibian tadpoles and in situ genotoxicity monitoring of aquatic environments with the alkaline single cell gel electrophoresis (comet) assay[J].Mut. Res.,1998,413:235-250[6] Croft B A, Brown AWA. Response of arthropod natural enemies to insecticides[J].A. Rev. Entomol.,1975,20:285-336[7] DeBach P, Rosen D. Biological control by natural enemies [M].London:Cambridge University Press,1991,271-273[8] Chen W L, He J L. A quick and sensitive method of detecting DNA damage-comet assay[J].The Foreign Medicine:the Hygiene.1998,25(2):101-104[陈玮琳,何继亮.快速敏感检测DNA断裂的方法-彗星试验.国外医学卫生学分册,1998,25(2):101-104][9] He J, Liu Y Q. The advancement and application of single cell gel electrophoresis assay [J].The Foreign Medicine: the Hygiene,1997,24(2):85-89[何惧,刘玉清.单细胞凝胶电泳技术的研究进展与应用.国外医学卫生学分册,1997,24(2):85-89][10] Verschaeve L, Gilles J. Single Cell Gel Electrophresis assay in the earthworm for the detection of genotoxic compounds in Soils [J].Bull. Environ. Contam. Toxicol.,1995,54:112-119[11] Wang M S, Scheemzer P. A introduce on the single cell gel electrophoresis assay [J].Carcinogenesis Teratogenesis and Mutagenesis,1996, 8(2):112[王民生,Schmezer, P.碱性单细胞微量凝胶电泳技术简介.癌变*畸变*突变,1996,8(2):112][12] Mitchelmore C L,Chipman, J K. DNA strand breakage in aquatic organisms and potential value of the comet assay in environmental monitoring [J].Mut. Res.,1998,399:135-147[13] Goethem F V, Lison D, Kirsch-Volders M. Comparative evaluation of in vitro micronucleus test and the alkaline single cell gel electrophoresis assay for the detection of DNA damaging agents: gents: genotoxic effects of cobalt powder, tungsten carbide and cobalt-tungsten carbide[J].Mut. Res.,1997,392:31-43[14] Collins A R, Ai-guo, M, Duthie S J. The kinetcs of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells [J].Mut. Res.,1995,336:69-77[15] Singh N P, Stephens R E. Microgel electrophoresis: Sensitivity, mechanisms, and DNA electrostretching [J].Mut. Res.,1997,383:167-175[16] Abd-Allah G A, El-Fayoumi R I, Smith M J, et al. A comparative evaluation of aflatoxin B1 genotoxicity in fish models using the Comet assay [J].Mut. Res.,1998,446:181-188[17] Feng S L, Lou Y, Zhong Y, et al. Single cell gel electrophoresis assay in the earthworm for measuring the genotoxicity of pesticides to DNA [J].Journal of Nanjing University(Natural Sciences),2000,36(5):649-652[封少龙,罗屿,钟远,等.应用单细胞凝胶电泳技术测定农药对蚯蚓的DNA损伤.南京大学学报(自然科学),2000,36(5):649-652][18] Li G, Chen X L, Chen D. et al. The application and studies of micronucleus assays with fish to screen mutagen in water [J].Acta Hydrobiologica Sinica.2002,26(2):74-81[李谷,程晓莉,陈丹,等.鱼微核试验筛检水体诱变物的应用与研究. 水生生物学报,2002,26(2):74-81][19] Li B. The advancement on the research and development of insecticide industry [J].Pesticides,2000,39(4):6-9[李斌.杀虫剂研发进展.农药,2000,39)4):6-9][20] Xu X L, Han L J, Wang X P, et al. Control Effect of Imidacloprid 20 SL in field to three aphides [J].Pestcides,2000,39(5):26-29[许小龙,韩丽娟,王小平,等.吡虫啉20SL新剂型对三种蚜虫的田间防治效果.农药,2000,39(5):26-29][21] Xuan R C, Wang Q Q, Zheng W, et al. Study on the adsorption of imidacloprid in soils and the interaction mechanism [J].Acta Scientiae Circumstantiae,2000,20(2):198-201[宣日成,王琪全,郑巍,等.吡虫啉在土壤中的吸附及作用机理研究.环境科学学报,2000,20(2):198-201][22] Jiang X Y, Wang K Y,Yi M J, et al. Studies on the Toxicity of Four species aphid and selectivity of natural enemy to synergistic imidacloprid[J].Pesticides,2000,39(9):26-27[姜兴印,王开运,仪美芹,等.增效吡虫啉对4种蚜虫的毒力和对天敌的选择性.农药,2000,39(9):26-27]
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