ZHAO Kai, YANG Gong-She, LI Jun-Bing, HE Shun-Ping. PHYLOGENETIC STRUCTURE OF SCHIZOPYGOPSIS PYL ZOVI POPULATIONS FROM MITOCHONDRIAL CYTOCHROME B GENE SEQUENCE VARIATIONS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 129-133.
Citation: ZHAO Kai, YANG Gong-She, LI Jun-Bing, HE Shun-Ping. PHYLOGENETIC STRUCTURE OF SCHIZOPYGOPSIS PYL ZOVI POPULATIONS FROM MITOCHONDRIAL CYTOCHROME B GENE SEQUENCE VARIATIONS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 129-133.

PHYLOGENETIC STRUCTURE OF SCHIZOPYGOPSIS PYL ZOVI POPULATIONS FROM MITOCHONDRIAL CYTOCHROME B GENE SEQUENCE VARIATIONS

  • Received Date: December 05, 2004
  • Rev Recd Date: September 22, 2005
  • Published Date: March 24, 2006
  • The complete mtDNA cytochrome b gene sequence (1141bp) was sequenced for 16 Schizopygopsis from the upper reach of the Yellow River and the Tuosuo Lake of the QaidamBasin.The sequence compositions and variations were analyzed using MEGA2.1 software.The Gymnocypris przewalskii and Gymnocypris eckloni and Platypharodon extremus were selected as outgroup,and one molecular phylogenetic tree of 5 haplotypes was constructed using NJ in PAPU 34.0b10 software.The monophyly of the populations of the Schizopygopsis from either the Yellow River or the Tuosuo Lake was not supported.The genetic variability (Fst) and the value of exact test of differentiation between the populations calculated with Arlequin Ver.2000 software were 01204(P0105) and 0.0532 (P0105), respectively.The Nm value deduced from Fst between the populations was 1195.These data suggested that the populations of the Schizopygopsis from the Yellow River and the Tuosuo Lake have not evolved to two different species.Our results support the morphological classification that merging the Schizopygopsis kessleri into Schizopygopsis pylzovi.The nucleotide diversity () exhibited by two populations was low (010012~010026).Inferred from the molecular evolution rate,the divergent time of the S.pylozovi from the Yellow River and the Tuosuo Lake was about 70000 years ago in the late Pleistocene.The faunal connection for population of the in historical was analyzed according to the phylogeography of population distributions and paleo2geographical events.
  • [1]
    Herzenstein S1Wissenschaftlichen Resultate der von N1M1Przewalskinach Central-Asien[J]Zool,Theil,Ⅲ,1891,2(3):181—262
    [2]
    Cao W X,W X W.An investigation of the fish biology and fishery probleme in Ganzi-Apa region of western Szechwan Province [J].Ac-ta Hydrobiologica Sinica.1962,(2):27—53[曹文宣,伍献文.四川西部甘孜阿坝地区鱼类生物学及渔业问题.水生生物学集刊,1962,(2):27—53].
    [3]
    Wu Y F,Wu C Z.The fishes of the Qingha-Xizang Plateau [M].Chengdu:Sichuan Science and Technology Press.1992 [武云飞,吴翠珍.青藏高原鱼类.成都:四川科学技术出版社,1992]
    [4]
    Cao W X. Schizothoracinae[A].In Wu XW etal.The Cyprinid fish-es of China.Vol.Ⅰ[M].Shanghai: Scientific and Technical Press.1964[曹文宣.裂腹鱼类亚科.中国鲤科鱼类志(上卷).上海:上海科学技术出版社.1964]
    [5]
    Cao W X,Chen Y Y,Wu Y F ,etal.Origin and evolution of Schizothoracine fishes in relation to the upheaval of the Xizang Plateau[A].In Collection in Studies on the Period ,Amplitude and Type of the Uplift of the Qinghai2Xizang Plateau [M](ed.The Team of the Comprehensive Scientific Expedition to the Qinghai-Xizang Plateau ,Chinese Academy of Sciences).Beijing:Science Press,1981,118—130[曹文宣,陈宜瑜,武云飞,等.裂腹鱼类的起源和演化及其与青藏高原隆起的关系.见:中国科学院青藏高原综合科学考察队.青藏高原隆起的时代、幅度和形式问题.北京:科学出版社,1981,118—130]
    [6]
    Perdices A,Cunha C,Coelih MM.Phylogenetic structure of Zacco platy-pus(Teleostei,Cyprinidae) populations on the upper and middle Chang Jiang(=Yangtze) drainage inferred from cytochrome b sequences [J].Molecular Phylogenetics and Evolution,2004,31:192—203
    [7]
    Falk T M,Teugels G G,Abban E K,etal.Phylogeographic patterns in populations of the black-chinned tilapia complex (Teleostei,Cichlidae) from coastal areas in West Africa:support for the refuge zone theory[J].Molecular Phylogenetics and Evolution 2003,27:81—92
    [8]
    Ausubel F M.Short Protocols in Molecular Biology[M].John Wiley,Sons 2ndeds.New York:1992
    [9]
    Thompson J D,Gibson TJ,Plewniak F,etal.The Clustal X windows interface:flexible strategies for multiple sequences alignment aided by Quality analysis tools[J].Nucleic Acids Res.,1997,25(24):4876—4882
    [10]
    Kumar S,Tamura K,Jakobsen B I,etal., MEGA:molecular evolutionary genetics analysis,Ver.210 [J].Bioinformatics,2001,17:1244—1245
    [11]
    Swofford D L.PAPU 3:Phylogenetic ananlysis using parsimony(3 and other methods),Ver 410[M].Sinauer Associates ,Sunderland Massachusetts,1998—2001
    [12]
    Excoffier L, Smouse P E,Quattor J M.Analysis if molecular variance inferred from metric distances among DNA haplotypes:application to human mitochondrial DNA restriction data [J].Genetics,2000,136:343—359
    [13]
    Hudson R R,Slatkin M,Maddison W P.Estimation of levels of gene flow from DNA sequence data[J].Genetics,1992,132:583—589
    [14]
    Raymond M,Rousset F.An exact test for population differentiation [J].Evolution,1995,49:1280—1283
    [15]
    Nei M.Molecular evolutionary genetics[M].New York:Columbia University Press,1987
    [16]
    Cantatore P,Roberti M,Pesole G,etal.Evolutionary analysis of cytochrome b sequences in some perciformes:evidence for a slower rate of evolution than in mammals[J].J.Mol.Evol.,1994,39:589—597
    [17]
    Kocher TD,Thomas W K,Meyer A,etal.Dynamics of mitochondrial DNA evolution in animals:Amplification and sequencingwith conserved primers[J].Proc.Natl.acad.Sci.USA,1989,86:6196—6200
    [18]
    Meyer A,Kocher T K,Bassasibwaki P.Monophyletic origin of Victoria cichlid fishes suggested by mitochondrial DNA sequences [J] .Nature,1990,347:550—553
    [19]
    Alves MJ,Coelho H,Collares-Pereira MJ,etal.Mitochondrial DNA variation in the highly endangered cyprinid fish Anaecypris hispanica:importance for conservation[J].Heredity,2001,87:463—473
    [20]
    Mesquita N,Carvalho G,Shaw P,etal.River basin-related genetic structuring in anendangered fish species,Chondrostoma lusitanicum,based on mtDNA sequencing and RFLP ananlysis [J].Heredity,2002,86:253—264
    [21]
    Billington N,Hebert P D N.Mitochondrial DNA diversity in fishes and its implications for introductions [J].Can.J.Fish.Aquat.Sci.,1991,48 (suppl.1):80—94

Catalog

    Article views (1285) PDF downloads (747) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return