ISOZYME ANALYSIS AND PRELIMINARY CONFIRMATION OF THE GENETIC MARKERS IN TWO ARTIFICIAL GYNOGENETIC POPULATIONS OF SILVER CARP, HYPOPHTHALMICHTHYS MOLITRIX

Two artificial gynogenetic populations were obtained from different broodstocks of silver carp, Hypophthalmichthys molitrix,originated from two fishery farms in Wuhan. The eggs of each broodstock were inseminated with UV-irradiated sperm of red common carp (Cyprinus carpio) and heat shock was performed through 39℃ for 1 min at 4 min after insemination for inducing diploid by inhibiting second polar body extrusion. Four isozyme zymograms, including esterase (EST), lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and superoxide dismutase (SOD),were analysed by polyacrylamide gradient gel electrophoresis in liver, muscle and red blood cells from the two artificial population silver carps. In comparison with the popular hatchery silver carps, there were more similar isozyme zymograms among the individuals of artificial gynogenetic silver carp, and therefore, there existed higher homogeneity because of gynogenesis. Additionally, obvious difference between the gynogenetic populations and the hatchery populations was found from the isozyme zymograms. Some bands specific to the different gynogenetic populations wereobserved in EST isozyme zymograms in liver and muscle. Therefore, the specific bands would be suggested as the genetic markers of the two artificial gynogenetic populations of silver carp.