THE EFFECTS OF Zn^2＋ STRESS ON THE GROWTH, PHYSIOLOGICAL CHARACTERISTICS AND CELL STRUCTURE OF CHLOROCOCCUM SP

QIU Chang-En; BI Yong-Hong; HU Zheng-Yu

Volume 31 Issue 4

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2007 > 31(4): 503-508.

QIU Chang-En, BI Yong-Hong, HU Zheng-Yu. THE EFFECTS OF Zn^2＋ STRESS ON THE GROWTH, PHYSIOLOGICAL CHARACTERISTICS AND CELL STRUCTURE OF CHLOROCOCCUM SP[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(4): 503-508.

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THE EFFECTS OF Zn^2＋ STRESS ON THE GROWTH, PHYSIOLOGICAL CHARACTERISTICS AND CELL STRUCTURE OF CHLOROCOCCUM SP

The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072;
2. The Department of Biology, Hubei Normal University, Huangshi 435002

Received Date: January 03, 2006
Rev Recd Date: January 12, 2007
Published Date: July 24, 2007

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ulture medium was served as the control. The results showed that Zn2+ affected markedly on the growth, physiological characteristics and cell structure of Chlorococcum sp. under certain concentration. When the concentration of Zn2+ was 0.1-1mg/L, the growth of Chlorococcum sp. showed no obvious difference compared with the control. When the concentration of Zn2+ was 10-50mg/L, Chlorococcum sp. could maintain certain growth rate yet. When the concentration of Zn2+ was higher than 100mg/L, the growth of Chlorococcum sp. was inhibited markedly. The contents of Chl a ＋ Chl b and Chl a decreased gradually with the increase of the concentrations of Zn2+ in the medium. When the concentration of Zn2+ was less than 10rag/L, the photosynthesis and respiration of Chlorococcum sp. increased gradually with the increase of Zn2+ concentrations; and when the concentration of Zn2+ was higher than 10mg/L, they decreased gradually with the increase of Zn2+ concentrations. The content of malondiadehyde and activity of peroxidase increased gradually with the increase of Zn2+ concentrations, and the activities of catalase and superox-ide dismutase increased at first and later decreased with the increase of Zn2+ concentrations. Compared with the cell cultured in BG11, the cell cultured in low concentrations (≤10mg/L) showed few changes in pigment and thickness of cell wall. The cell wall of cell cultured in high concentrations of Zn2+ became thicker, and the pigment decreased and the pyrenoid disappeared. When the concentration of Zn2+ was≤10mg/L, the removal rate of Chlorococcum sp. on Zn2+ reached the maximum and was 100%, and it was above 80% under the concentrations that Chlorococcum sp. could maintain the growth. The results demonstrated that the Chlorococcum sp. could be applied to the treatment of wastewater containing Zn2+, because the Chlorococcum sp. could endure the stress of Zn2+ and was of high removal rate on Zn2+.
- Chlorococcum sp.,
- Zn2+,
- Growth,
- Physiological characteristic,
- Cell structure

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[1]	Bake M D, M ayfied C I, Inniss W E. Toxicity of pH, heavy metals and bisulphate to a freshwater green algae[J]. Chemosphere, 1983,12(1): 35)44
[2]	Travieso L, Caizares K O, Borja K, et al. Heavy metal removal by microalgae[J]. Bulletin of Environmental Contamination And Tox-icology, 1999, 62: 141)151
[3]	Valdman E, Leite S G F. Biosorption of Cd, Zn and Cu by S arga-ssum sp. waste biomass[J]. Bioprocess Engineering, 2000, 22:171)173
[4]	Vymazal J. Shor-t term uptake of heavy metals by periphyton algae[J]. Hydrobiologic, 1984, 119: 171)179
[5]	Sheng P X, Ting Y P, Chen J P, et al. Sorption of Lead, Copper,Cadmium, Zinc and Nickel by marine algal biomass: characterization of biosorptive capacity and investigation of mechanisms[J]. Journal of Colloid and Interf ace Science, 2004, 275: 131)141
[6]	Chong A M Y, Wang Y S, Tam N F Y. Performance of different mi-croalgal species in removing Nickel and Zinc from industrial wastewater[J]. Chemosphere, 2000, 41: 251)257
[7]	Tang Z C. The experimental guide of modern plant physiology[M].Beijing: Academic Press. 1999, 95, 305, 314[汤章城主编. 现代植物生理学实验指南. 北京: 科学出版社. 1999, 95, 305,314]
[8]	Li H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press. 2000, 164)165[李合生主编. 植物生理生化实验原理和技术. 北京: 高等教育出版社. 2000, 164)165]
[9]	Chen S Y. Injury of menbrane lipid peroxidation to plant cell[J].Pl ant Physiology Communicati ons, 1991, 27(2): 84)90[陈少裕.膜脂过氧化对植物细胞的伤害. 植物生理学通讯, 1991,27(2): 84)90]
[10]	Kuang Q J, Xia Y C, Hui Y. Toxic effects of heavy metals on algae[J]. Acta Hydrobiologica Sinica, 1996, 20 (3): 277)283[况琪军, 夏宜, 惠阳. 重金属对藻类的致毒效应. 水生生物学报, 1996, 20(3): 277)283]
[11]	Sicko G L. A morphometric analysis of algae response to low dose,short term heavy metal exposure[J]. Protoplasma, 1982, 110:75)86
[12]	Dai L F, Gao H, Xia J R. Tolerance to heavy metal Cd and detoxif-ication of Synechococcus cedrorum[J]. Chin. J. Appl. Environ.Biol.,1998, 4(3): 192)195[戴玲芬, 高宏, 夏建荣. 雪松聚球藻对重金属镉的抗性和解毒作用. 应用与环境生物学报,1998, 4(3): 192)195]
[13]	Pistocchi R, Mormile A M, GuerriniF, et al. Increased production of extra-and intracellular meta-l ligands in phytoplankton exposed to cop-per and cadmium[J]. Journal of Applied phycology, 2000, 12:469)477

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