CONSTRUCTION OF INTESTINAL cDNA LIBRARY AND ANALYSIS OF SOME EXPRESSED
SEQUENCE TAGS SEQUENCING OF CTENOPHARYNGODON IDELLUS

ZHANG Xue-Jun; QU Gang; ZHU Wen-Li; ZHANG Li; WANG Juan; WU Jiang; LIU Han-Yuan; CHEN Fang; XU Heng

Volume 31 Issue 2

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2007 > 31(2): 251-258.

ZHANG Xue-Jun, QU Gang, ZHU Wen-Li, ZHANG Li, WANG Juan, WU Jiang, LIU Han-Yuan, CHEN Fang, XU Heng. CONSTRUCTION OF INTESTINAL cDNA LIBRARY AND ANALYSIS OF SOME EXPRESSED SEQUENCE TAGS SEQUENCING OF CTENOPHARYNGODON IDELLUS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 251-258.

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CONSTRUCTION OF INTESTINAL cDNA LIBRARY AND ANALYSIS OF SOME EXPRESSED SEQUENCE TAGS SEQUENCING OF CTENOPHARYNGODON IDELLUS

Key Laboratory of Bio-resources and Eco-environment(Ministry of Education), College of Life Science, Sichuan University, Chengdu 610064;
2. Tongwei Group Co. Ltd, Chengdu 610081

Received Date: July 11, 2006
Rev Recd Date: December 09, 2006
Published Date: March 24, 2007

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Abstract

Abstract

The technology of expressed sequence tag ( EST) was broadly applied in analysis of gene function and expression pattern. Based on the construction technology of non-normalized cDNA library, the cDNA libraries of grass carpps intestinal cDNA Library was constructed. This library reaches 2. 3 *105in capacity; the percenyage of recombination is as high as 95%. PCR results showed that the average size of inserts was larger than 1000 bp. And a total of 1571 successful sequencing reactions yielded 1411 which expressed sequence tags ( ESTs) longer than 100 bp. In addition, 939 non-repetitive sequences were formed including 188 contigs and 751 singletons from these ESTs after initial assembly. BasicLocal Alignment Search Tool (BLAST) analysis showed that nearly 44.5% of the sequences were assigned putative functions because they showed similarity to known genes (E-valueCtenopharyngodon idellus.
- Ctenopharyngodon idellus,
- Intestine,
- cDNA library,
- Expressed Sequence Tag,
- Sequencing

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References

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CONSTRUCTION OF INTESTINAL cDNA LIBRARY AND ANALYSIS OF SOME EXPRESSED SEQUENCE TAGS SEQUENCING OF CTENOPHARYNGODON IDELLUS

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