WU Hou bo, PAN Jin pei. THE IMMUNE PROTECTIVE EFFECTS OF THREE KINDS OF VACCINATION AGAINST VIBRIOSIS IN MARINE-CAGE CULTURED RED SEA BREAM(PAGRUS MAJOR)[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 457-464.
Citation: WU Hou bo, PAN Jin pei. THE IMMUNE PROTECTIVE EFFECTS OF THREE KINDS OF VACCINATION AGAINST VIBRIOSIS IN MARINE-CAGE CULTURED RED SEA BREAM(PAGRUS MAJOR)[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 457-464.

THE IMMUNE PROTECTIVE EFFECTS OF THREE KINDS OF VACCINATION AGAINST VIBRIOSIS IN MARINE-CAGE CULTURED RED SEA BREAM(PAGRUS MAJOR)

  • Received Date: September 24, 2001
  • Rev Recd Date: December 24, 2001
  • Published Date: September 24, 2002
  • Red sea bream(Pagrus major)were injected intramuscularly and direct immersion with Vibrio mimicus formalin killed cells)FKC), crude lipopolysaccharide(LPS)preparations and formalinized extracellular toxin(toxoid)of V. mimicus , respectively. The crude LPS prepared by the phenol water method had a little toxicity. The crude LPS was being heated to reduce its toxicity. The effectiveness of vaccination against Vibrio mimicus were evaluated two weeks after the immunization. The results showed that good protections were induced in red sea bream by FKC, LPS and toxoid. After booster immunization, the degrees of protection were enhanced.
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    吴后波,潘金培.海水网箱养殖真鲷弧菌病病原生物学[C]. 迈向21世纪的渔业科技创新. 北京:海洋出版社,2000, 672-677[2] Whistiler R L. Methods in carbohydrate chemistry Vol V, New York: Academis Pdress, 1965, 83-91[3] Dubois M, Furer E, Sandoff J.C. et al. Colorimetric methods for th e determination of sugars and related substances [J].Anal Chem, 1956,28(2): 350-356[4] 吴后波,潘金培.海水养殖真鲷弧菌病病原菌外毒素的分离、纯化及生物学活性[J]. 海洋与湖沼(已接受)[5] Baxa D V, Kawai K, Kusuda R. In vitro and in vivo activities of Flexibacter maritimus toxins[J].Rep. Usa Mar. Bio. Inst., Kochi Univ., 198 8, 10:1-8[6] Hastings T S, Ellis A E. Detection of antibodies induced in rainbow trout by different Aeromonas salmonicida vaccie preparations[J]. Journa l of Aquatic Animal Health, 1990, 2:135-140[7] Lund V, Jorgensen T, et al. Humoral immune response in Atlantic Sal mon,Salmo Salar, to cellular and extracellular antigens of Aeromonas salmo nicida[J].Journal of Fish Disease, 1991 14:443-452[8] Arnesen J A, Bjornsdottir R, et al. Immunological responses in Atla ntic Salmon, Salmo Salar L, against purified serine protease and haemolysins from Aeromonas salmonicida[J]. Journal of Fish Disease, 1993, 16:409-423[9] 陈昌福.草鱼对鱼害粘球菌类脂多糖的免疫反应[J].淡水渔业,1989, 4:3-5

    吴后波,潘金培.海水网箱养殖真鲷弧菌病病原生物学[C]. 迈向21世纪的渔业科技创新. 北京:海洋出版社,2000, 672-677[2] Whistiler R L. Methods in carbohydrate chemistry Vol V, New York: Academis Pdress, 1965, 83-91[3] Dubois M, Furer E, Sandoff J.C. et al. Colorimetric methods for th e determination of sugars and related substances [J].Anal Chem, 1956,28(2): 350-356[4] 吴后波,潘金培.海水养殖真鲷弧菌病病原菌外毒素的分离、纯化及生物学活性[J]. 海洋与湖沼(已接受)[5] Baxa D V, Kawai K, Kusuda R. In vitro and in vivo activities of Flexibacter maritimus toxins[J].Rep. Usa Mar. Bio. Inst., Kochi Univ., 198 8, 10:1-8[6] Hastings T S, Ellis A E. Detection of antibodies induced in rainbow trout by different Aeromonas salmonicida vaccie preparations[J]. Journa l of Aquatic Animal Health, 1990, 2:135-140[7] Lund V, Jorgensen T, et al. Humoral immune response in Atlantic Sal mon,Salmo Salar, to cellular and extracellular antigens of Aeromonas salmo nicida[J].Journal of Fish Disease, 1991 14:443-452[8] Arnesen J A, Bjornsdottir R, et al. Immunological responses in Atla ntic Salmon, Salmo Salar L, against purified serine protease and haemolysins from Aeromonas salmonicida[J]. Journal of Fish Disease, 1993, 16:409-423[9] 陈昌福.草鱼对鱼害粘球菌类脂多糖的免疫反应[J].淡水渔业,1989, 4:3-5

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