PRELIMINARY STUDY ON THE SUSCEPTIBILITY OF GOBIOCYPRIS RARUS TO HEMORRHAGIC VIRUS OF GRASS CARP(GCHV)

This is the report on the susceptibili ty of Gobiocypris rarus to Hemorrhagic Virus ofGrass Carp(GCHV). The hemorrhage of GobiocJlpris rarus is induced by the GCHV in theage from 1-6 months when the water temperature is 22℃-32℃. At the water temperatureof 28℃, the diseased fish die in one day, whose latent period is 5 days after infection, The period of high mortality of the diseased fish comes in 6-8 days after the infection, GCHV cantransmit in Gobiocypris rarus, and more than 80% of the fish die from the infection ofGCHV. The electron microscopic examination on the uItrathin sections reveals that there arevirus particles, which resemble GCHV in size and shape, in such organs as the intestinaltract, the spleen, and the kidney etc. of the artifficially infected hemorrhagic fish. Theimmuno-electron microscopic observation showed that the purified virus particles isolatedfrom the hemorrhagic Gobiocypris raru,,can be aggregated by the specific GCHVantibody. The resuIts prove that the hemorrhage of Gobiocypris rarus is caused by GCHV,and that Gobiocypris rarus is susceptible to GCHV, Furthermore, Gobiocy Pris rarus mayserve as a model of tlie Virus-resistance breeding of Grass Carp with biotechnique methods.