Volume 25 Issue 6
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PAN Qian-sheng, FANG Zhi-ping. THE TMMUNOCYTOCHEMICAL EVIDENCE FOR TWO HORMONES IN THE GASTROINTESTINAL ENDOCRINE CELLS OF MANDARIN FISH[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 565-570.
Citation: PAN Qian-sheng, FANG Zhi-ping. THE TMMUNOCYTOCHEMICAL EVIDENCE FOR TWO HORMONES IN THE GASTROINTESTINAL ENDOCRINE CELLS OF MANDARIN FISH[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 565-570.
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THE TMMUNOCYTOCHEMICAL EVIDENCE FOR TWO HORMONES IN THE GASTROINTESTINAL ENDOCRINE CELLS OF MANDARIN FISH

  • Huazhong Agricultural University, Wuhan 430070

  • Received Date: May 17, 1999
  • Rev Recd Date: January 17, 2001
  • Published Date: November 24, 2001
    Graphical Abstract
    • Abstract
  • By using immunocytochemical method of Strept Avidin-Biotin-Complex(SABC), 4 kinds of antisera raised against rabbit hormones were used to identify and locate the endocrine cells in the gastrointestinal tract of mandarin fish(Siniperca chuatsi). Results showed that glucagon(Glu)-and gastrin(Gas)-immunoreactive endocrine cells were not found in the gastrointestinal tract of mandarin fish; somatostatin(Som)-, and serotonin(5-HT)-immunoreactive endocrine cells were found in the cardia, pylorus and gut of mandarin fish in varying degrees. There were distributions of Som-immunoreactive endocrine cells between epithelium cardia and cardiac gland, and between epithelium pylorus and pyloric gland; their distribution in the cardiac gland and pyloric gland was denser than in the epithelium cardia and pylorus. There were more 5-HT-immunoreactive endocrine cells in the fundus vertriculi and intestinal epithelial tissue. Also there were more 5-HT-immunoreactive endocrine cells in the neurones of nerve plexus in muscle layer. These peptidergic neurones contained serotonin have the typical characteristic of APUD(Amine Precursor Uptake and Decarboxylation)cells. It is a prompt that as in the mamalian also there are many peptidergic neurones in the gastrointestinal tract of fish. It provided morphological basis for the research of neuro-endocrine system.
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    • References (14)
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