ISOLATION ANDPRELIMINARY STUDY OF ARHABDOVIRUS FROMRAINBOW TROUT(ONCORHYNCHUS MYKISS)IN BENXI, CHINA

In April 1990,an acute infectious disease broke out among the rainbow trout fry atBenxi Rainbow Trout Fam,causing a nearly 100% loss of trout fry,Aviral agent wasisolated from rainbow trout fry in the farm. It can replicate in the rainbow trout gonadcells(RTG-2), chinook salmon embryo cells(CHSE)and fathead minnow cells(FHM).A maximum titer of 106.3 TCID50/0.1mlwas attained in the CHSE cells.Theoptimum temperature of replication was 15℃, Plaques can be fomed in CHSEmonolayer cells measuring 0.5-1.5mm in diameter. The agent is unstable to chloroformtreatment and does not resist heat or acid. Electron microscopy revealed rhabdovinlsparticles measuring 58-91nm in diameter and 131-173nm in length.The results showedthat this virus is probably an infectious hematopoietic necrosis virus(IHNV).