氮磷营养限制影响三角褐指藻光合无机碳利用和碳酸酐酶活性
EFFECTS OF NITROGEN OR PHOSPHORUS LIMITATION ON PHOTOSYNTHETIC INORGANIC CARBON UTILIZATION AND CARBONIC ANHYDRASE ACTIVITY IN PHAEODACTYLUM TRICORNUTUM
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摘要: 以海洋硅藻三角褐指藻为实验材料, 研究了不同氮磷比培养对其光合无机碳利用和碳酸酐酶活性的影响, 结果显示三角褐指藻生长速率在N:P=16:1时最大, 高于或低于16:1时明显下降, 表明其最适生长受到氮磷的限制。氮限制(N:P=4:1或1:1)导致叶绿素a含量分别下降30.1% 和47.6%, 磷限制(N:P=64:1或256:1)下降39.1%和52.4%, 但氮或磷限制对叶绿素c含量并没有明显影响。不同营养水平培养对光饱和光合速率具有明显的影响, 与营养充足培养相比, 在严重氮磷限制(N:P=1:1或256:1)培养下光饱和光合速率分别下降39.7%和48.0%, 光合效率与暗呼吸速率也明显下降。在氮磷限制培养下藻细胞pH补偿点明显下降; K0.5CO2值在磷限制下降低30%, 表明磷限制有助于提高细胞对CO2的亲和力, 但氮限制并没有明显影响。在氮磷限制培养的细胞反应液中Fe (CN)63-浓度下降速率较慢, 表明在氮磷限制环境中生长的细胞质膜氧化还原能力明显低于营养充足条件下生长的细胞。氮磷限制也导致胞内、外碳酸酐酶活性明显下降, 其中在氮限制下胞外碳酸酐酶活性分别下降50%和37.5%, 在磷限制下下降22.3%和42.1%。严重的氮(N:P=1:1)或磷(N:P=256:1)限制导致胞内碳酸酐酶活性下降36.5%和42.9%。研究结果表明, 三角褐指藻细胞在氮磷营养限制的环境中, 可以通过调节叶绿素含量、无机碳的利用方式和碳酸酐酶的活性以维持适度的生长。Abstract: Influences of nitrogen (N) and phosphorus (P) ratios on photosynthetic inorganic carbon utilization and carbonic anhydrase activity in marine diatom Phaeodactylum tricornutum were investigated. The results showed that the growth rate in the algae grown under the medium with N:P=16:1 was the largest. Higher or lower than N:P=16:1 resulted in a significant decrease in growth rate, which indicated that the optimum growth was limited by nitrogen or phosphorus supply. Chlorophyll a content was reduced by 30.1% and 47.6% in N-limited cells (N:P=4:1 or 1:1), by 39.1% and 52.4% in P-limited cells (N:P=64:1 or 256:1), however N or P limitation showed no significant affect on the content of chlorophyll c. Compared with nutrient-replete cultures, severe N or P-limited cultures (N:P=1:1 or 256:1) resulted in a decrease by 39.7% and 48.0% in light-saturated photosynthetic rate. Nutrient-limited conditions also caused a significant decline in photosynthetic efficiency and dark respiration rate. K0.5CO2 decreased by 30% in P-limited conditions, which indicated P limitation could increase the affinity of the algal cell for CO2. Plasma membrane redox rate was lower in nutrient limitation than that in nutrient replete. Extracellular carbonic anhydrase activity was reduced by 50% and 37.5% in N-limited condition, by 22.3% and 42.1% in P-limited conditions relative to nutrient-replete conditions. Intracellular carbonic anhydrase activity was 36.5% and 42.9% lower in severe N or P limitation than in nutrient-replete cultures. The above results showed that this alga grown under conditions of nutrient limitation (N or P) could maintain a moderate growth by adjusting chlorophyll content, the mode of inorganic carbon utilization and carbonic anhydrase activity.
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Keywords:
- Phaeodactylum tricornutum /
- N or P limitation /
- Photosynthesis /
- Carbonic anhydrase
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