THE INHIBITORY EFFECT OF HONOKIOL ON AEROLYSIN SECRETION BY AEROMONAS HYDROPHILA
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摘要: 为了研究不同浓度和厚朴酚对嗜水气单胞菌(Aeromonas hydrophila)致病力的影响, 筛选抗嗜水气单胞菌感染的天然化合物, 通过溶血试验、免疫印记试验、荧光定量PCR试验和动物试验进行了研究。结果发现, 和厚朴酚能在亚抑菌浓度下降低嗜水气单胞菌培养物上清中的溶血活性; 蛋白免疫印迹试验发现和厚朴酚能降低嗜水气单胞菌气溶素的分泌; 荧光定量PCR试验进一步表明和厚朴酚与嗜水气单胞菌共培养后降低了气溶素编码基因aerA的转录而降低气溶素的分泌。此外, 通过动物试验发现和厚朴酚治疗能显著提高斑点叉尾鮰( Ictalurus punctatus )嗜水气单胞菌感染模型的存活率。以上研究表明, 和厚朴酚能通过降低气溶素编码基因aerA的转录而降低嗜水气单胞菌的致病力, 和厚朴酚是一种潜在的新型抗嗜水气单胞菌感染的先导化合物。Abstract: To investigate the effects of honokiol on the pathogenicity of Aeromonas hydrophila and identify novel drugs against infections caused by A. hydrophila, hemolytic assay, Western-Blotting, real-time PCR and animal assay were performed. The results showed that honokiol could inhibit the hemolytic activity of A. hydrophila by inhibiting the expression of aerolysin in a co-culture model with sub-inhibitory concentrations. The real-time PCR results revealed that honokiol could suppress the transcription of aerolysin encoding gene aerA. Moreover, honokiol treatment could significantly increase the survival rate of the channel catfish infected with A. hydrophila. These results demonstrate that honokiol could reduce the pathogenicity of A. hydrophila by inhibiting the transcription of aerA gene, and that Honokiol is a promising candidate of the novel drug against A. hydrophila infections.
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Keywords:
- Aeromonas hydrophila /
- Aerolysin /
- Honokiol /
- Infection
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图 1 和厚朴酚与嗜水气单胞菌共培养后的生长曲线
Figure 1. Growth curves of A. hydrophila co-cultured with different concentrations of honokiol
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图 2 和厚朴酚与嗜水气单胞菌XS-91-4-1共培养上清的溶血活性
Figure 2. Hemolytic activity of supernatants of A. hydrophila XS-91-4-1 co- cultured with honokiol
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图 3 嗜水气单胞菌培养物上清中气溶素的表达量
Figure 3. Expression of aerolysin in A. hydrophila supernatant
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