阳光紫外辐射对室内水培发状念珠藻生理特性的影响
EFFECTS OF SOLAR ULTRAVIOLET RADIATION ON PHYSIOLOGICAL CHARACTERISTICS OF THE AQUATIC-LIVING COLONIES OF NOSTOC FLAGELLIFORME CULTURED INDOOR
-
摘要: 发状念珠藻(Nostoc flagelli forme Bornet & Flahault)是一种重要的陆生经济蓝藻,室内培育出的原植体如何适应阳光辐射的问题尚需探讨。为此,作者将室内水培发菜置于阳光下培养,测定了其生长、有效光化学效率(ΔF/F′m)和色素的变化。结果表明,较高的可见光(PAR,395-700nm)和紫外辐射(UVR,280-395nm)均导致水培发菜的ΔF/F′m下降。第1天中午,PAR和UVR分别使ΔF/F′m下降了54%和13%;傍晚,ΔF/F′m有部分恢复。UVR对发菜适应阳光2d后的生长无负面作用。发菜在适应全阳光辐射期间,紫外吸收物质(Scytonemin和Mycosporine-like amino acids)含量不断增加,9d后,分别增加了124倍和9倍。这些紫外吸收物质的增加对发菜细胞降低光抑制,适应阳光辐射,起到了重要作用。本研究的结果可为水培发菜室外培养方法的建立提供一定的理论依据。Abstract: Nostoc flagelliforme Bornet & Flahault, named popularly as 'Facai' in Chinese, is a terrestrial cyanobacterium of great economic value. Although aquatic-living colonies were developed under indoor conditions, little is known whether they could and how they would adapt to solar radiation. Therefore, we exposed the colonies to different solar radiation treat-ments, photosynthetically active radiation (P:PAR,395-700 nm), PAR+ultraviolet radiation-A (PA,320-700 nm) or PAR+total ultraviolet radiation (PAB,280-700 nm). Changes of their growth, effective quantum efficiency (△F/F'm) and pigments were estimated during the experimental period. It was found that high levels of both PAR and ultraviolet radiation (UVR,280-395 nm) reduced the △F/F'm of the colonies. △F/F'm was reduced about 54% by PAR and further declined 13% by UVR. The △F/F'm recovered in the late afternoon. After two days' acclimation to solar radiation, the biomass of the colonies increased during the following days. UV-absorbing compounds, scytonemin and mycosporine-like amino acids (MAAs), increased by 124 and 9 times, respec-tively, in 9 days. Such increased UV-absorbing substances played an important role in protecting the aquatic-living colo-nies of Nostoc flagelliforme from solar UVR. The results may be useful for the mass production of the aquatic-living colo-nies of Nostoc flagelliforme under outdoor conditions.
-
-
[1] Hu H J,Li R Y,Wei Y X,et al.Freshwater Algae in China[M].Shanghai:Shanghai Scientific and Technological Publishing House.1980,49-56[胡鸿钧,李尧英,魏印心,等.中国淡水藻类.上海:上海科学技术出版社.1980,49-56]
[2] Diao Z M.Study of natural conditions and ecological physiology characteristics for growth of Nostoc flagelliforme in Qinghai Province[J].Chinese J Ecol,1996,15(1):8-13[刁治民.青海发菜生长的自然条件及其生态生理学特性研究.生态学杂志,1996,15(1):8-13]
[3] Gao K,Qiu B,Xia J,et al.Light dependency of the photosynthetic recovery of Nostoc flagelliforme[J].J Appl Phycol,1998,10:51-53
[4] Qiu B,Gan K.Dried field populations of Nostoc flagelliforme (Cyanophyceae) require exogenous nutrients for their photosynthetic recovery[J].J Appl Phycol,1999,11:535-541
[5] Gao K,Zou D.Photosynthetic bicarbonate utilization by a terrestrial cyanobacterium, Nostoc flagelliforme (Cyanophyceae)[J].J Phycol,2001,37:768-771
[6] Gao K,Yu A.Influence of CO2,light and watering on growth of Nostoc flagelliforme mats[J].J Appl Phycol,2000,12:185-189
[7] Gao K,Ye C.Culture of the terrestrial cyanobacterium,Nostoc flagelliforme (Cyanophyceae),under aquatic conditions[J].J Phycol,2003,39(3):617-623
[8] Bi Y H,Hu Z Y.Influence of temperature,nutrients and light intensity on the growth of Nodtoc flagelliforme[J].The ChineseJournal of Process Engineering,2004,4(3):245-249[毕永红,胡征宇.温度、营养和光强对发状念珠藻生长的影响.过程工程学报,2004,4(3):245-249]
[9] Kanekiyo K,Lee J B,Hayashi K,et al.Isolation of an antiviral polysaccharide,nostoflan,from a terrestrial cyanobacterium,Nostoc flagelliforme[J].J Nat Prod,2005,68:1037-1041
[10] Huang Z,Liu Y,Paulsen B S,et al.Studies on polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies:comparison of monosaccharide compositions and viscosities of polysaccharides from field colonies and suspension cultures[J].J Phycol,1998,34:962-968
[11] Wang M,Xu Y N,Jiang G Z,et al.Membrane lipids and their fatty acid composition in Nostoc flagelliforme cells[J].Acta Botanica Sinica,2004,2(12):1263-1266[王梅,许亦农,姜桂珍,等.发菜膜脂及其脂肪酸组成.植物学报,2004,2(12):1263-1266]
[12] Huang H,Zhong Z P,Wang K B,et al.Isolation and characterization of the cytoplasmic membrane from the terrestrial cyanobacterium Nostoe flagelliforme[J].Acta Botanica Sinica,2004,46(10):1186-1191
[13] Buhimann B,Bossard P,Uehlingar U.The influence of longwave ultraviolet radiation (u.v.-A) on the photosynthetic activity (14C-assimilation) of phytoplankton[J].J Plankton Res,1987,9(5):935-943
[14] Quesada A,Vincent W F.Strategies of adaptation by antarctic cyanobacteria to ultraviolet radiation[J].Eur J Phycol,1997,32:335-342
[15] Wu H,Gao K,K,illafane V E,et al.Effects of solar UV radiation on morphology and photosynthesis of filamentous cyanobacterium Arthrospira platensis[J].Appl Environ Microbiol,2005,71(9):5004-5013
[16] Stanier R.Y.,Knnisawa R.,Mandel M,et al.Purification and properties of unicellular blue-green algae (order Chrooeoccales)[J].Bacteriol Rev,1971,35(2):171-205
[17] Figueroa F L,Salles S,Aguilera J,et al.Effects of solar radiation on photoinhibition and pigmentation in the red alga Porphyra leucosticta[J].Mar Ecol Prog Ser,1997,151:81-90
[18] van Kooten O,Snel J F H.The use of chlorophyll fluorescence nomenclature in plant stress physiology[J].Photosynth Res,1990,24(3):147-150
[19] Porra R J.The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b[J].Photosyrah Res,2002,73:149-156
[20] Ehling-Schulz M,Bilger W,Scherer S.UV-B-inducad synthesis of photnprotective pigments and extracellular polysaccharides in the terrestrial cyanobacterium Nostoc commune[J].J Bacteriol,1997,179(6):1940-1945
[21] Hader D P,Lebert M,Marangoni R,et al.ELDONET-European Light Dosimeter Network hardware and software[J].J Photochem Photobiol B:Biol,1999,52(1):51-58
[22] Osmond C B,Chow W S.Ecology of photosynthesis in the sun and shade:summary and prognostications[J].Aunt J Plant Physiol,1988,15:1-9
[23] Garcia-Pichel F,Wingard C E,Castenholz R W.Evidence regarding the UV sunscreen role of a mycesparine-like compound in the cyanobacterium Gloeocapsa sp[J].Appl Environ Microbiol,1993,59(1):170-176
[24] Gao K,Ye C.Photosynthetic insensitivity of the terrestrial cyanobacterium Nostoc flagelliforme to solar UV radiation while rehydratad or desiccated[J].J Phycol,2007,43(4):628-635
[25] Buckley C E,Houghton J A.A study of the effects of near UV radiation on the pigmentation of the blue-green alga Gloeocapsa alpicola[J].Arch Microbiol,1976,107:93-97
[26] Steiger S,Schafer L,Sandmann G.High-light-dependent upregulation of carotenoids and their antioxidative properties in the cyanobacterium Synechocystis PCC 6803[J].J Photochem.Photobiol B:Biol,1999,52(1):14-18
[27] Garcia-Pichel F,Sherry N D,Castenholz R W.Evidence for an ultraviolet sunscreen role of the extracallular pigment scytonemin in the terrestrial cyanobacterium Chlorogloeopsis sp.[J].Photochem Photobiol,1992,56(1):17-23
[28] Garcia-Picbel F,Castenholz R W.Characterization and biological implications of scytonemin,a cyanobacterial sheath pigment[J].J Phycol,1991,27:395-409
[29] Franklin L A,Krabs G,Kuhlenkamp R.Blue light and UV-A radiation control the synthesis of mycesporine-like amino acids in Chondrus crispas (Floridcophyceae)[J].J Phycol,2001,37(2):257-270
-
期刊类型引用(7)
1. 樊莹, 邹丰才, 董霞, 匡海鸥. 雌性东方蜜蜂基因表达差减文库的构建及初步分析. 云南农业大学学报(自然科学). 2015(05): 710-721 . 
百度学术
2. 杨波, 刘红, 蔡生力, 方昱. 中国明对虾(Fenneropenaeus chinensis)卵黄发生前期与消退期卵巢和肝胰腺消减文库的初步构建及分析. 广东农业科学. 2013(14): 138-143 . 百度学术
3. 赵大显, 陈敏文, 江坤, 吴小平. 嗜水气单胞菌诱导中华绒螯蟹消减文库的构建及初步分析. 江西农业大学学报. 2013(02): 398-403 . 百度学术
4. 郜卫华, 迟淑艳, 谭北平, 刘泓宇, 董晓慧, 杨奇慧. 低渗诱导凡纳滨对虾肝胰腺消减文库的构建及其表达序列标签分析. 广东海洋大学学报. 2012(06): 1-9 . 百度学术
5. 殷勤, 崔亮, 彭金霞, 韦嫔媛, 谢达祥, 陈秀荔, 王志伟, 李奎, 陈晓汉. 凡纳滨对虾ANT2基因的克隆及低温表达谱分析. 水生生物学报. 2012(01): 24-28 . 本站查看
6. 褚椒江, 朱鹏, 严小军. 海带藻际微生物抗菌活性及其多样性的初步研究. 水生生物学报. 2011(06): 972-979 . 本站查看
7. 李会琴, 林炜铁, 蔡小龙, 李敬源, 唐水水. 对虾养殖水环境宏基因组Fosmid文库的构建. 生物技术通报. 2011(06): 112-115+126 . 百度学术
其他类型引用(4)
计量
- 文章访问数: 1118
- HTML全文浏览量: 0
- PDF下载量: 643
- 被引次数: 11
下载: