植酸酶和非淀粉多糖酶对鲈鱼生长和消化酶活性的影响

EFFECTS OF PHYTASE AND NON2STARCH POLYSACCHARI DE ENZYM ESUPPLEM ENTATI ON IN D IETS ON GROW TH AND D IGESTIVE ENZYM E ACTIVITY FOR JAPANESE SEABASS, LATEOLABRAX JAPON ICUS C.

  • 摘要: 以初始体重为(6.26±0.10)g鲈鱼(Lateolabr axjaponicus Cuvier)为研究对象,探讨饲料中添加植酸酶(PY)和非淀粉多糖酶(WX和VP)(WX为木聚糖酶和葡聚糖酶的复合酶制剂,VP为纤维素酶、半纤维素酶、葡聚糖酶和果胶酶的复合酶制剂)对鲈鱼生长、体组成以及胃和肠道消化酶活性的影响.以含36.1%鱼粉和18.0%复合蛋白(豆粕:肉骨粉:花生粕:菜籽粕=4:3:2:1并添加赖氨酸、蛋氨酸和异亮氨酸以模拟鱼粉必需氨基酸含量)的饲料为基础饲料(Diet1),分别向每千克基础饲料中添加200mg PY(Diet2)和400 mg VP+800mg WX(Diet3),配制出3种等氮(粗蛋白43%)等能(20kJ/g)的实验饲料.实验在1.5m×1.5m×2.0m的浮式海水网箱中进行,每个处理设3个重复,每个重复放养60尾鲈鱼.每天饱食投喂2次(06:00和18:00).实验期间水温为27.5-29.5℃,盐度为25‰-28‰,溶解氧大于7mg/L.实验结果表明饲料中添加外源酶对鲈鱼的成活率无显著影响(92%-95%),但却显著提高了鲈鱼的增重百分率(从859.3%提高到947.2%和905.2%)和特定生长率(从4.0提高到4.2和4.1)(pppp<0.01).

     

    Abstract: A feeding experiment was conducted to investigate the effects of dietary exogenous enzymes (Phytase, non-starch polysaccharide enzymes: WX and VP) on growth, body composition and digestive enzymes activitives of Japanese seabass, Lateolabrax japonicus C1 (initialweight (61-6 ± 0110) g) 1 A basal dietwas used as the control (Diet1), which contained 3611% fish meal and 1810% compound protein source (soybean meal: meat and bone meal: peanut meal: rape-seed meal=4: 3: 2: 1, and crystalline lysine, methionine and isoleucinewere supplemented to simulate the essential amino acid profile of fish meal) 1 Two other dietswere supplemented with-00mg phytase and the combination of 800mgWX and 400 mgVP per kilogram diet, respectively1 Each dietwas fed to triplicate groups of fish in seawater floating cages (115m &#-15; 115m &#-15;-10m), and each cage was stocked with 60 fish1 Fish were fed twice daily (06: 00 and 18: 00) to apparent satia-tion for 8 weeks1 The water temperature fluctuated from-715℃ to-915℃, the salinity from-5‰ to-8‰ and dissolved oxygen content was above 7mg/L during the experimental period1 Results showed that exogenous enzymes supplementa-tion did not significantly influence the survival of Japanese seabass (9-%-95%), but significantly improved the growth (weight gain (WG) increasing from 85913% to 9471-% and 9051-%, specific growth rate (SGR) from 410 to 41-and 411%/d) (p p p p <0101), but no significant differences in lipase and amylase were observed.

     

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