植酸酶和非淀粉多糖酶对鲈鱼生长和消化酶活性的影响
EFFECTS OF PHYTASE AND NON2STARCH POLYSACCHARI DE ENZYM ESUPPLEM ENTATI ON IN D IETS ON GROW TH AND D IGESTIVE ENZYM E ACTIVITY FOR JAPANESE SEABASS, LATEOLABRAX JAPON ICUS C.
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摘要: 以初始体重为(6.26±0.10)g鲈鱼(Lateolabr axjaponicus Cuvier)为研究对象,探讨饲料中添加植酸酶(PY)和非淀粉多糖酶(WX和VP)(WX为木聚糖酶和葡聚糖酶的复合酶制剂,VP为纤维素酶、半纤维素酶、葡聚糖酶和果胶酶的复合酶制剂)对鲈鱼生长、体组成以及胃和肠道消化酶活性的影响.以含36.1%鱼粉和18.0%复合蛋白(豆粕:肉骨粉:花生粕:菜籽粕=4:3:2:1并添加赖氨酸、蛋氨酸和异亮氨酸以模拟鱼粉必需氨基酸含量)的饲料为基础饲料(Diet1),分别向每千克基础饲料中添加200mg PY(Diet2)和400 mg VP+800mg WX(Diet3),配制出3种等氮(粗蛋白43%)等能(20kJ/g)的实验饲料.实验在1.5m×1.5m×2.0m的浮式海水网箱中进行,每个处理设3个重复,每个重复放养60尾鲈鱼.每天饱食投喂2次(06:00和18:00).实验期间水温为27.5-29.5℃,盐度为25‰-28‰,溶解氧大于7mg/L.实验结果表明饲料中添加外源酶对鲈鱼的成活率无显著影响(92%-95%),但却显著提高了鲈鱼的增重百分率(从859.3%提高到947.2%和905.2%)和特定生长率(从4.0提高到4.2和4.1)(pppp<0.01).Abstract: A feeding experiment was conducted to investigate the effects of dietary exogenous enzymes (Phytase, non-starch polysaccharide enzymes: WX and VP) on growth, body composition and digestive enzymes activitives of Japanese seabass, Lateolabrax japonicus C1 (initialweight (61-6 ± 0110) g) 1 A basal dietwas used as the control (Diet1), which contained 3611% fish meal and 1810% compound protein source (soybean meal: meat and bone meal: peanut meal: rape-seed meal=4: 3: 2: 1, and crystalline lysine, methionine and isoleucinewere supplemented to simulate the essential amino acid profile of fish meal) 1 Two other dietswere supplemented with-00mg phytase and the combination of 800mgWX and 400 mgVP per kilogram diet, respectively1 Each dietwas fed to triplicate groups of fish in seawater floating cages (115m &#-15; 115m &#-15;-10m), and each cage was stocked with 60 fish1 Fish were fed twice daily (06: 00 and 18: 00) to apparent satia-tion for 8 weeks1 The water temperature fluctuated from-715℃ to-915℃, the salinity from-5‰ to-8‰ and dissolved oxygen content was above 7mg/L during the experimental period1 Results showed that exogenous enzymes supplementa-tion did not significantly influence the survival of Japanese seabass (9-%-95%), but significantly improved the growth (weight gain (WG) increasing from 85913% to 9471-% and 9051-%, specific growth rate (SGR) from 410 to 41-and 411%/d) (p p p p <0101), but no significant differences in lipase and amylase were observed.
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Keywords:
- Lateolabrax japonicus C. /
- Phytase /
- Non-starch polysaccharide enzymes /
- Digestive enzyme /
- Growth
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[1] Arndt R E, Hardy R W, Sugiura S H, et al. Effects of heat treatment and substitution level on palatability and nutritional value of soy defatted flour in feeds for coho salmon, Oncorhynchus kisutch [J]. Aquaculture, 1999, 180: 129-145
[2] Webster C D, Goodgame L S, Tidwell J H. Total replacement of fish meal, with various percentage of supplemental Lmethionine, in diets for blue catfish, Ictalurus furcatus (Lesueu) [J]. Aqua. Res., 1995, 26: 299-306
[3] Lee SM. Apparent digestibility coefficientsof various feed ingredients for juvenile and grower rockfish (Sebaste schlegeli) [J]. Aquaculture, 2002, 207: 79-95
[4] AiQ H, Xie X J. Effects of replacement of fish meal by soybean meal and supplementation of methionine in fish meal/soybean meal2based diets on growth performance of the southern catfish Silurus meridionalis [J]. J. W orld Aqua. Soc., 2005, 36: 498-507
[5] Tacon A G J, Jackson A. Utilization of conventional and unconventional protein sources in practical fish feeds [A]. In: Cowey C B, Mackie A M M, Bell J G (Eds.), Nutrition and Feeding in fish [C]. London: Acedemic Press. 1985, 211-264
[6] BaiD Q, Qiao X T, Wei D, et al. Effect of phytase on utilization ratio of nutrient composition (Calcium, Phosphorus, etc.) of carp (Cyprinus carpio L.) [J]. Journal of Tianjin Agriculture College, 2003, 10: 6-10 [白冬清,乔秀亭,魏东,等. 植酸 磷对鲤钙磷等营养物质利用率的影响. 天津农学院学报, 2003, 10: 6-10]
[7] NRC (National Research Council). Nutrient requirements of Fish [M]. Washington D. C.: NationalAcademy Press. 1993, 14
[8] Bedford M B, Classen H I. Reduction of intertional viscosity throughmainpulation of dietary rey and pentosanase concentration is effected through changes in carcarbohydrate: composition of the aqueous phase and results in improved growth rate and food conversion efficiency in chicks [J]. J Nutr., 1991, 122: 560-569
[9] Zhang C X, Mai K S, Ai Q H, et al. Dietary phosphorus requirement of juvenile Japanese seabass, Lateolabrax japonicas [J]. Aquaculture, 2006, 258: 535-542
[10] AiQ H, Mai K S, ZhangC X, et al. Effectsof dietary vitamin C on growth and immune response of Japanese seabass, Lateolabrax japonicas [J]. Aquaculture, 2004, 242: 489-500
[11] AiQ H, Mai K S, LiH T, et al. Effectsof dietaryprotein to energy ratios on growth and body composition of juvenile Japanese seabass, Lateolabrax japonicus [J]. Aquaculture, 2005, 230: 507-516
[12] AOAC, Official methods of Analysis of AOAC International [M]. Vol. I. Agriculture Chemical; Contaminants, Drug. 16th edn. AOAC International, Arlington, VA. 1995
[13] Pan J X. Technical research of Protein chemical [M]. Beijing: Science Publishing Company. 1962[潘家秀. 蛋白质化学研究 技术. 北京: 科学出版社. 1962]
[14] YettyN, Roshada H, Ahyaudin A, et al. Characterization of digestive enzymes in a carnivorous ornamental fish, the Asian bony tongue Scleropages for mosus (Osteoglossidae) [J]. Aquaculture, 2004, 233: 305-320
[15] PanL Q, Wang KX. The experimental studyon activityof digestive enzyme in the larvae Penaeus Chinensis [J]. Journal of Fisheries of China, 1997, 21 (1): 26-31 [潘鲁青,王克行. 中国对虾幼体消化酶活力的实验研究. 水产学报, 1997, 21 (1): 26-31]
[16] Lanari D E, Agaro D, Turri C. Use of nonlinear regression to evaluate the effects of phytase enzyme treatment of plant protein diets for rainbow trout (Oncorhynchus mykiss) [J]. Aquaculture, 1998, 161: 345-356
[17] Oliva2TelesA, Pereira J P, Gouveia A, et al. Utilization of diets supplemented with microbial phytase by seabass (Dicentrarchus labrax) juveniles [J]. Aquat Liv. Resour, 1998, 11 (4): 255-259
[18] Kenneth Cain KD, Donald GarlingD C. Pretreatmentof soybean meal phytase for salmonid diets to reduce phosphorus concentrations in effluents [J]. Prog. Fish Cult, 1995, 57 (1): 114-119
[19] Xu J X, CuiL, ChenL Y. The effectofNPS enzyme supplemented to corn2soybean diet on growth and digestibility of weanling pigs [J]. Journal of ShanghaiUniversity (agriculture science), 2001, 19 (1): 9-1[徐建雄, 崔立, 陈鲁勇. 玉米 豆粕型 日粮添加非淀粉多糖酶对仔猪早期生长和消化性能的影响. 上海交通大学学报, 2001, 19(1): 9-12]
[20] Xu X R, Lu J J. Effect of exogenous enzymes (Xylanase, β2glucanase and cellulose) on structure and Function of digestive tract in growing pig fed paddy2based diets [J]. Scientia Agriculture Sinica, 2003, 36(2): 201-207 [许辛荣,卢建军. 稻谷型日 粮添加非淀粉多糖酶对生长猪消化道结构和功能的作用研 究. 中国农业科学, 2003, 36(2): 201-207]
[21] Vielma J, Ruohonen K, PeiskerM. Dephytinization of two soy proteins increases phosphorus and protein utilization by rainbow trout, Oncorhynchus mykiss [J]. Aquaculture, 2002, 204: 145-156
[22] Jackson L S, LiM H, Robinson E H. Use of microbial phytase in channel catfish Ictalurus punctatus diets to improve utilization of phytate phosphorus [J]. J. W orld Aqua. Soc., 1996, 27: 309-313
[23] Babkin B P. Secretary mechanis m of the digest glands [M], New York, Paul, B, Hoeber, Inc., 1963, 54
[24] Tian L X, Lin D. Changes in digestive enzyme activities of grass carp Ctrnopharyngodon Idellus after feeding on two test diets [J]. Acta Hydrobiologica Sinica, 1993, 17(1): 58-65 [田立 霞,林鼎. 草鱼摄食两种蛋白质饲料后消化酶活性变动比 较. 水生生物学报, 1993, 17(1): 58-65]
[25] Zhou J X, Chen Y, HuangQ, et al. On activity of digestive Ferment of fish and its change affected by circumstance [J]. Journal of Beihua University (Natural Science), 2001, (1): 70-74 [周景祥,陈勇,黄权,等. 鱼类消化酶的活性及环境条件 的影响. 北华大学学报 (自然科学版), 2001, (1): 70-74]
[26] Lin H R. Fish physiology [M]. High Education Publishing Company of Guangdong. 1999 [林浩然. 鱼类生理学. 广东高 等教育出版社. 1999]
[27] Li J, He R G, Wang X D. Digestive enzyme activity distribution in digestive system ofChinese sturgeon [J]. China Feed, 2002, 21: 18-20 [李谨,何瑞国,王学东. 中华鲟消化系统内消化 酶活性分布. 中国饲料, 2002, 21: 18-20]
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