Abstract: In combination with the scanning of CS 910 doublewave-length scanner, the hemoglobins （Hb） and sera of hybrid Yue-carp and its parents were analyzed with the polyacrylamide gel disc electrophoresis. The following results were obtained: ⅰ). Both hybrid Yue-carp and its parents had the similar electrophoretic spectra of Hb. ⅱ). The sera between the parents were different in some major and minor bands, ⅲ). The hybrid Yue-carp had the same major bands of serum as that of paternal fish in B1 area and maternal fish in B2 area, except for one major band. ⅳ). The differences between sexes and among individuals were also detectable.From above, it Seems justified that serum can be used as a genetic marker to distinguish the male and female parents, as well as the parents and their hybrid during the process of fish breeding.