DNA条形码技术的研究进展及其应用
THE PROGRESS AND APPL ICATION OF DNA BARCOD ING
-
摘要: DNA条形码技术(DNA Barcoding)是通过对一个标准目的基因的DNA序列进行分析从而进行物种鉴定的技术。这个概念的原理与零售业中对商品进行辨认的商品条形码是一样的。简单地说, DNA条形码技术的关键就是对一个或一些相关基因进行大范围的扫描,进而来鉴定某个未知的物种或者发现新种[ 1-3 ]。自从提出DNA条形码的概念以来,这种新兴分类学技术已经引起了越来越多的生物学家的关注。DNA条形码技术是分类学中辅助物种鉴定的新技术,它代表了生物分类学研究的一个新方向[ 4 ],因此它在生态、环境、食品等诸多领域都将会有广泛的应用[ 5 ]。本文概括综述了DNA条形码技术的发展历史、原理与操作,分析了其在生物分类中的应用及应用上的优势与限制,对DNA条形码技术在鱼类学研究的意义与可行性进行了探讨。
-
Keywords:
- DNA Barcoding /
- Taxonomy /
- Species identification
-
-
[1] Hebert P D N, Cywinska A, Ball S L, et al. Biological identification through DNA barcodes [J]. Proc R Soc Lond B B iol Sci,2003, 270: 313-321
[2] Hebert P D N, StoeckleM Y, Zemlak T S, et al. Identificationof birds through DNA barcodes [J]. PLOS B iol, 2004, 2 (10) :1657-1663
[3] Hebert P D N, Ratnasingham S, deWaard J R. Barcoding animallife: cytochrome c oxidase subunit 1 divergences among closelyrelated species [J]. Proc R Soc Lond B B iol Sci, 2003, 270:96-99
[4] Xiao J H, Xiao H, HuangDW. DNA: barcoding: new app roachof biological taxonomy [J]. Acta Zoologica S inica, 2004, 50(5) : 852-855
[5] Janzen D H. Now is the time [J]. Phil. Trans. R. Soc. B,2004, 359: 731-732
[6] Wang X Z. Molecular phylogeny of the East Asian cyp rinids( Pisces: Cyp riniformes) [M]. Thesis forDoctor of Science. In2stitute of Hydrobiology, Chinese Academy of Sciences,Wuhan. 2005
[7] StoeckleM. Taxonomy, DNA, and the barcode of life [J]. B io2science, 2003, 53 (9) : 2-3
[8] VencesM, ThomasM, Bonett R M, et al. Deciphering amphibi2an diversity through DNA barcoding: chances and challenges [J]. Phil. Trans. R. Soc. B, 2005, 360: 1859-1868
[9] VencesM, ThomasM, Meijden A V, et al. Comparative per2formance of the 16S rRNA gene in DNA barcoding of amphibians [J]. Frontier in Zoology, 2005, 2 (5) : 1-12
[10] Janzen D H, HajibabaeiM, Burns JM. Wedding biodiversity in2ventory of a large and comp lex lep idop tera fauna with DNA bar2coding [J]. Phil. Trans. R. Soc. B, 2005, 360: 1835-1845
[11] Ward R D, Zemlak T S, InnesB H, et al. DNA barcodingAus2tralia’s fish species [J]. Phil. Trans. R. Soc. B, 2005, 360:1847-1857
[12] HajibabaeiM, Janzen D H, Burns J M, et al. DNA barcodesdistinguish species of trop ical Lep idop tera [J]. PNAS, 2006,103: 968-971
[13] Chase M W, Salamin N, Wilkinson M, et al. Land p lants andDNA barcodes: short2term and long2term goals [J]. Phil.Trans. R. Soc. B, 2005, 360: 1889-1895
[14] Saunders GW. App lying DNA barcoding to red macroalgae: ap reliminary app raisal holds p romise for future app lications [J].Phil. Trans. R. Soc. B, 2005, 360: 1879-1888
[15] Avise J C, WalkerD. Pleistocene phylogeographic effects on avi2an populations and the speciation p rocess [J]. Proc R Soc LondB B iol Sci, 1998, 265: 457-463
[16] Weckstein J D, Zink R M, Blackwell2Rago R C, et al. Anoma2lous variation in mitochondrial genomes of White2crowned( Zonotrichia leucophrys) and Golden crowned ( Z. atricapilla )sparrows: Pseudogenes, hybridzation, or incomp lete lineage so2ring [J]. Auk, 2001, 118: 231-236
[17] Lovette I J, Bermingham E. Mitochondrial perspective on thephylogenetic relationship s of the Parula wood2warblers [J].Auk, 2001, 118: 211-215
[18] Bensasson D, ZhangD X, HartlD L, et al. Mitochondrial p seu2dogenes: Evolutionpsmisp laced witnesses [J]. Trends Ecol Evol,2001, 16: 314-321
[19] Ballard J W O, WhitlockM C. The incomp lete natural history ofmitochondriap [J]. M ol Ecol, 2004, 13: 729-744
[20] Johnson N K, Cicero C. New mitochondrialDNA data affirm theimportance of Pleistocence speciation in North American birds [J]. Evolution, 2004, 58: 1122-1130
[21] Gregory T R. DNA barcoding does not compete with taxonomy [J]. N ature, 2005, 434: 1067
[22] Ebach CM, Holdrege C. DNA barcoding is no substitute for tax2onomy [J]. N ature, 2005, 434: 697
[23] Wiens J J, Penkrot TA. Delimiting species usingDNA and mor2phological variation and discordant species limits in sp iny lizards(Sceloporus) [J]. Syst B iol, 2002, 51: 69-91
[24] Bensasson D, ZhangD X, HartlD L, et al. Mitochondrial p seu2dogenes: Evolutionpsmisp laced witnesses [J]. Trends Ecol Evol,2001, 16: 314-321
[25] Witt J D S, TherloffD L, Hebert P D N. DNA barcoding revealsextraordinary cryp tic diversity in an amphipod genus: imp licationsfor desert sp ring conservation [J]. M ol Ecol, 2006, 15: 3073-3082
[26] StoeckleM. Taxonomy, DNA and the barcode of life [J]. B io2science, 2003, 53: 2-3
[27] Savolainen V, Cowan R S, VoglerA P, et al. Towardswriting theencyclopedia of life: an introduction to DNA barcoding [J].Phil. Trans. R. Soc. B, 2005, 360: 1805-1811
[28] Moritz C, Cicero C. DNA bracoding: promise and p itfalls [J].PLOS B iol, 2004, 2 (10) : 28-29
[29] Avise J C. Phylogeography: The history and formation of species [M]. Cambridge (Massachusetts) : Harvard University Press.2000, 447
计量
- 文章访问数: 1272
- HTML全文浏览量: 10
- PDF下载量: 897
下载: