Abstract: γ-tubulin is a newly discovered protein, which is considered to be essentialfor microtubule nucleation.With the help of the polymerase chain reaction(PCR) weamplified and sequenced the γ-tubulin gene of Euplotes octocarinatus, a hypotrichousciliate.Polyclonal antibody to localize mis protein in E.octocarinatus and found, thatγ-tubulin is present in the basal bodie of dordal cilia,the basal bodies of cirri and the basal bodies of membranelles.In addition,it was found in the micronucleus and andin the macronucleus.