载铜蒙脱石对尼罗罗非鱼肠道菌群和消化机能的影响
EFFECTS OF COPPER-BEARING MONTMORILLONITE ON INTESTINAL MICROFLORA AND DIGESTIVE FUNCTION OF NILE TILAPIA(OREOCHROMIS NILOTICUS)
-
摘要: 本试验研究了饲粮中添加0.10%、0.15%、0.20%载铜蒙脱石对尼罗罗非鱼(Oreochromis niloticus)肠道菌群和消化机能的影响。结果显示,载铜蒙脱石显著降低了肠道细菌数量,明显改变了肠道菌群的组成,使气单胞菌属、假单胞菌属、黄杆菌属、不动杆菌属、产碱菌属、肠杆菌科、弧菌属的百分比减少。与对照组相比,饲料中添加0.10%、0.15%和0.20%载铜蒙脱石分别使干物质消化率显著提高了6.43%、8.47%和8.83%(pppppp<0.05)。载铜蒙脱石显著提高了前肠、中肠、后肠绒毛和微绒毛高度。结果提示,载铜蒙脱石在鱼体内具有较强的抗菌活性,能有效抑制肠道病原菌的增殖,保护肠粘膜免受病原菌的侵袭,使肠粘膜始终处于健康状态,以益于消化酶的分泌和饲料养分的消化。Abstract: A total of 360 Nile tilapia at an average initial body weight of 3.9g were randomly allocated to 4 treatments,each of which had three replicates of 30 fish/tank and used to investigate the effects of copper-bearing montmorillonite(Cu-MMT) on intestinal microflora and digestive function.The fish received the same basal diet and Cu-MMT was added to the basal diet at 0,0.10%,0.15% and 0.20%,respectively.As compared with the control,supplementation with Cu-MMT significantly reduced the total intestinal aerobic bacterial counts,affected the composition of intestinal microflora with a tendency of Aeromonas,Pseudomonas,Flavobacterium,Acinetobacter,Alcaligence,Enterobacteriaceae and Vibrio decreasing.Addition of 0.10%,0.15% and 0.20% Cu-MMT significantly improved the apparent digestibility coefficients of dry matter,crude protein and crude lipid and by 6.43%,8.47% and 8.83%,5.63%,6.24% and 6.91%,4.62%、5.65% and 5.98%,respectively(pppp<0.05).Cu-MMT also increased villus and microvillus heights at different sites of the intestine.The results suggest that Cu-MMT can inhibit effectively the growth and proliferation of pathogenic bacteria in the intestinal tract,which can protect intestinal mucosa from invasion of pathogenic bacterium and toxins,resulting in a positive effect on the health and function of intestinal mucosa,and then promote the digestion and absorption of dietary nutrition.
-
-
[1] Ma Y L, Xu Z R. Advan ces in Ion-type Inorgan ic Ant ibacterialMaterials [J]. M ateria lsR eview, 2004, 18: 16-18, 48 [马玉龙,许梓荣. 离子型无机抗菌材料研究进展. 材料导报, 2004,18: 16-18, 48]
[2] H u C H, X ia M S. An tib acterial effect of Copper-bearing m on -tm orillon ite on aquacu ltural pathogen ic b acteria and d iscuss ion onits m echan ism [J]. Journal of th e Chinese C eram ic Society,2005, 3: 1375-1379 [胡彩虹, 夏枚生. 载铜蒙脱石对水产病原菌的抗菌活性及其机理. 硅酸盐学报, 2005, 3: 1375-1379]
[3] H u C H, Xu Z R, X ia M S. An tibacterial effect of Cu2+ -exchangedm on tm ori llon ite on Aerom onas hyd roph ila and d iscu ss ionon itsm echan ism [J]. Ve tM icrobiol, 2005, 109: 83-88
[4] H u C H, X iaM S, X iong L, et a l. E f fects of Cu bearing m on -tm orillon ite on Aerom onas hydroph ila adhesion to epithelial cellsof N ile tilap ia [J]. Jou rnal of F isheries of China, 2005, 29:619-623 [胡彩虹, 夏枚生, 熊莉, 等. 载铜蒙脱石对嗜水气单胞菌粘附尼罗罗非鱼上皮细胞的影响. 水产学报, 2005, 29:619-623]
[5] Nat ional Research Council ( NRC ). Nutrient Requ irem en ts ofF ish [M]. W ash ington, DC: Nat ionalA cadem y Press. 1993
[6] Dong X Z, Ca iM Y. M anual of System atically Determ inativeBacteriology [M]. Bei jing: S cience Pub lish ing C ompany. 2001 [东秀珠, 蔡妙英. 常见细菌系统鉴定手册. 北京: 科学出版社. 2001]
[7] H olt JG, K rieg NR, Sn eath P H A, et a l. BergeypsM anual ofDeterm in at ive Bacteriology [M]. W illiam s & W i lk ins, Balt-imore. 1993
[8] Ye JD, Lu T Y, L iu H B, et a l. E ffect of olaqu indox on d igestive en zym e act ivities and phagocytos is of leucocytes in Cyprinuscarp io L. [J]. Journal of Da lian F ish eries Un iversity, 2004, 19:161-166 [叶继丹, 卢彤岩, 刘红柏, 等. 喹乙醇对鲤的消化酶活性及白细胞吞噬功能的影响. 大连水产学院学报,2004, 19: 161-166]
[9] W icken A J. Bacteria l adh es ion: M ech an ism and physiolog icals ign ifican ce. 2. B acterial cell w al ls and su rfaces [M]. NewYork: P lenum Press. 1985, 45-47
[10] Guo T. S tudy on th e m echan ism s of adsorpt ive and an tib acterialact ivity against intestin alp athogen of Cu-exchanged sil icate n anoparticles in w ean ling p igs. P. D [D]. ZhejiangUn ivers ity, 2004,105-107 [郭彤. 载铜硅酸盐纳米微粒对断奶仔猪肠道病原菌吸附、杀菌机理的研究. 博士学位论文. 浙江大学, 2004,105-107]
[11] L iu H B, Zh ang Y, Yang Y H, e t a l. E ffects of five Ch in eseherb m ed icines as addit ive in feed on the grow th and intestin alm icrof lora in comm on carp ( Cyprinu s carp io) [J]. Journal ofDa lian F isheries Un iv ersity, 2004, 19( 1) : 16-20 [刘红柏, 张颖, 杨雨辉, 等. 5种中草药作为添加剂对鲤肠内细菌及生长的影响. 2004, 19 ( 1) : 16-20]
[12] G irardeau J P. Smectite aggregation by Escherich ia coli [J]. A ctaG astroen terolog ica B elg ica, 1987, 50: 181-192
[13] H u X R, L?G L, C hen L S, et al. S tudy on the m echan ism ofthe in teract ion betw een m ontmorillon ite and b acterium [J]. A ctaP harm aceu tica S in ica, 2002, 37: 718-720 [胡秀荣, 吕光烈,陈林深, 等. 天然蒙脱石与细菌相互作用机理的研究. 药学学报, 2002, 37: 718-720]
[14] W ang J X, Fang H S. In test inal m icroecological observat ion ofsm ectite in the treatm en t of d ietary in ch ild ren [J]. Ch in J C linP harm acol, 1995, 11: 134-137 [王俊侠, 方鹤松. 思密达治疗腹泻患儿肠道微生态学观察. 中国临床药理学杂志,1995, 11: 134-137]
[15] Tang F S, ZhuX Y, Zhang X Z. Th e in fluences of comm on carpintestin al bacteria and its am ylases on the host d iges tion [J].Journa l of F isheries of China, 1994, 18: 177-182 [汤伏生, 朱晓燕, 张兴忠. 鲤鱼肠道细菌及其淀粉酶对宿主消化的影响.水产学报, 1994, 18: 177-182]
[16] Tauqir N A, N aw azH. Perform ance and econ om ics of bro ilerch icks fed on rat ion s supplem ented w ith d ifferent levels of sod iumben ton ite [J]. In t. J. Agri. B iol., 2001, 3: 149-150
[17] Venglovsky J, Paca jova Z, Sasakova N, et a l. Adsorpt ion p roperties of natural zeo lite and b enton ite in pig slu rry from th em icrobiological poin t of view [J]. Veterinarni M ed icina, 1999, 44:339-344
[18] H u C H, Xu Y, X ia M S, et a l. E ffects of Cu2+ -exchangedmontmorillon ite on grow th perform an ce, m icrobial eco logy and intestinalm orphology of N ile t ilapia ( Oreoch rom is niloticu s) [J].A quacu lture, 2007, 270( 1) 4 ) : 200-206
[19] D ing S L, Peng S P, L iu Q P, e t al. Analys is of feasib il ity bentonite as feed ing addit ive [J]. Journa l of L iaon ing T echn ica lUniversi ty (Na tu ral S cien ce Ed ition ), 2003, 22: 23-26 [丁述理, 彭苏萍, 刘钦甫, 等. 膨润土用作饲料添加剂可行性分析.辽宁工程技术大学学报, 2003, 22: 23-26]
[20] Le S, Y inY Z, Ge R. Iso lation and characterization of f ishA eromonas hyd roph ila adhesin s important for in vitro ep ith elial cell invasion [J]. J F ish D isease s, 1997, 20: 169-175
[21] N an jing MedicalU n ivers ity. B ig thesauru s of Ch in ese trad itionalm ed icine [M]. Shangh a:i Sh anghai S cience& T echnology Publishing Com pany. 1991 [江苏新医学院. 中药大辞典. 上海: 科学技术出版社. 1991]
[22] Zhao X H. The new u se of sm ectite [J]. M odern Journal of Integrated T rad i tiona l Chinese and W estern M ed icin e, 2003, 12: 2263 [赵秀红. 思密达临床新用途. 现代中西医结合杂志, 2003,12: 2263]
[23] A lbengres E S, Urien J P, T illem ent P, et al. In teractions between sm ectite, a m ucus stab ilizer, and acid ic and basic d rugs [J]. Eu r J C lin Pharmacol, 1985, 28: 601-605
[24] Droy-Lefain M T, D rouet Y and Schatz B. Sod ium glycodeoxycholateand sp inab ility of gastro intestinalm ucus: Protect ive ef fectof sm ectite [J]. Ga stroenterol, 1985, 88 ( Supp.l 2) : 13-69
-
期刊类型引用(18)
1. 瞿飞虎，姜晓娜，葛彦龙，翟钰姗，张玲，王继瑶，寇寒，李池陶，胡雪松，石潇丹，贾智英. 黄河鲤对饲料蛋白质需求量研究. 南方水产科学. 2024(04): 124-132 . 
百度学术
2. 易婉婷，华雪铭，颜克涛，宓水潮，蓝汉冰，杭莹，丛湘明. 酵母水解物与小肽对大口黑鲈幼鱼生长性能、肝肠健康与抗感染能力的影响. 上海海洋大学学报. 2022(05): 1097-1107 . 百度学术
3. 曾本和，刘海平，王建，王万良，周建设，王金林，朱成科，张忭忭. 饲料蛋白质水平对拉萨裸裂尻鱼幼鱼肌肉氨基酸及蛋白质代谢的影响. 中国水产科学. 2019(06): 1153-1163 . 百度学术
4. 徐兆利，曾本和，杨瑞斌，牟振波，李宝海，王万良，张忭忭，刘海平. 饲料蛋白质水平对拉萨裸裂尻鱼幼鱼免疫和抗氧化能力的影响. 动物营养学报. 2019(12): 5645-5654 . 百度学术
5. 赵书燕，林黑着，黄忠，周传朋，王珺，王芸，戚常乐. 不同蛋白水平对2种规格石斑鱼生长性能、血清生化及肌肉品质的影响. 南方水产科学. 2017(04): 87-96 . 百度学术
6. 孙金辉，范泽，张美静，程镇燕，白东清，乔秀亭. 饲料蛋白水平对鲤幼鱼肝功能和抗氧化能力的影响. 南方水产科学. 2017(03): 113-119 . 百度学术
7. 孔纯，华雪铭，杨璐，刘韬，杨景丰，王坛，王刚，吴钊，施永海，税春，苏美英. 暗纹东方鲀饲料中豆粕替代鱼粉的营养生理效应及其与大豆抗原蛋白的相关性. 水产学报. 2017(05): 734-745 . 百度学术
8. 李宗升，吴立新，李思萌，彭博文，赵博文. 鸡肉粉替代鱼粉对大菱鲆生长和体组成的影响. 水产科学. 2016(05): 486-491 . 百度学术
9. 王荻，刘红柏. 施氏鲟抗热应激中草药免疫制剂的筛选. 大连海洋大学学报. 2016(06): 635-639 . 百度学术
10. 吴金平，刘伟，蒋明，文华，吴凡，田娟，杨长庚. 大规格吉富罗非鱼幼鱼对饲料中胆碱的需要量. 动物营养学报. 2016(01): 256-264 . 百度学术
11. 王坛，华雪铭，朱伟星，吴钊，孔纯，何亚丁，苏美英. 饲料溶菌酶添加水平对氨氮应激下吉富罗非鱼血清生化指标、抗菌性能和肝脏抗氧化能力的影响. 水产学报. 2016(05): 740-750 . 百度学术
12. 王荻，廉超，刘红柏. 中草药添加剂对施氏鲟生长及血液生化指标的影响. 水产学杂志. 2016(03): 16-20 . 百度学术
13. 王震，徐玮，麦康森，路凯，刘迎隆，艾庆辉. 饲料缬氨酸水平对军曹鱼鱼体脂肪含量、血浆生化指标和肝脏脂肪代谢基因表达的影响. 水生生物学报. 2016(04): 744-751 . 本站查看
14. 吴金平，文华，蒋明，刘伟，吴凡，田娟，黄凤，任春. 生物素对吉富罗非鱼幼鱼生长、体成分及血清生化指标的影响. 西北农林科技大学学报(自然科学版). 2016(02): 15-22 . 百度学术
15. 胡田恩，王玲，张春晓，宋凯，李金宝. 饲料中添加胆汁酸对牛蛙生长性能、体组成和营养物质表观消化率的影响. 水生生物学报. 2015(04): 677-685 . 本站查看
16. 张晓雁，杜浩，危起伟，张艳珍，王彦鹏，蔡经江，刘志刚，乔新美，熊伟. 养殖中华鲟的产后康复. 水生生物学报. 2015(04): 705-713 . 本站查看
17. 李剑，刘淑兰，史庆超，翟少伟. 饲料中添加槲皮素对吉富罗非鱼幼鱼生长性能、血清生化指标和非特异性免疫指标的影响. 饲料工业. 2014(16): 21-25 . 百度学术
18. 范兴，陆玉芹，陈名帅，颜明月，陈德慰. 三卡因甲磺酸盐麻醉对罗非鱼离水保活的影响. 食品科技. 2014(09): 174-179 . 百度学术
其他类型引用(16)
计量
- 文章访问数: 1019
- HTML全文浏览量: 1
- PDF下载量: 510
- 被引次数: 34
下载: