应用微卫星标记分析野生中国明对虾的亲权关系
KINSHIP ANALYSIS IN THE CHINESE SHRIMP ( FENNEROPENAEUS CHINENSIS) BASE ON MICROSATELLITE DNA MARKERS
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摘要: 利用5个高度多态性的微卫星座位(Fc04、Fc06、Fc18、Fc24、Fc27),分析了不同年份中建立的10个野生中国明对虾家系中的雌性亲虾及其后代基因型分离的情况,表明每个家系中只有一个雄性野生中国明对虾对子代的遗传有贡献。从其中3个家系的雌性亲虾纳精囊中提取了雄虾精子DNA,微卫星标记显示各家系的子代个体均有一个等位基因与雄性亲虾的基因型相符,且符合孟德尔遗传规律,这为野生中国明对虾雌虾在繁殖季节一对一的繁殖行为提供了遗传学的证据。用UPMGA的方法随机对3个家系的40尾子代个体进行聚类分析,每个家系都能被单独聚成一类。上述工作表明微卫星标记在对虾育种中作为一种有效的亲子关系分析工具,在种群的遗传结构、亲缘关系、繁殖行为等方面具有广阔的应用前景。
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关键词:
- 微卫星 /
- 中国明对虾(Fenneropenaeus chinensis) /
- 亲权分析
Abstract: The mating mode of Chinese shrimp ( F. chinensis) is supposed to be pair mating from observation of their matinghabits. However,this suggestion has not been confirmed genetically yet. In this study,five microsatellite loci ( Fc04, Fc06, Fc18,Fc24 and Fc27) were used to survey the mating mode of the wild Chinese shrimp in ten families which were built in differentyears. The male’s genotypes could be determined fromthose of a female and her offspring at every locus.Only one combination ofmale genotypes was observed in each family. Farther,the spermDNAof wild male was extracted from copulated wild female thelycum in three families of the ten. The result of microsatellite markers showed that the sperm DNA of wild males were not contami2nated by female genome DNA.At each locus,every progeny had one allele found in the male genotype. The distributions of geno2types in offspring of the ten families were consistent with the Mendelian segregation.All of these suggested that one female copu2lated to one male and supported that the mating mode of this prawn is pair2mating. The cluster analysis based on UPMGA (unweighted pair2group method using arithmetic averages) between individual within/ among families was also examined and showedthat each family formed single cluster,respectively.Microsatellite markers as an effective tool for kinship analysis could be widelyused to reveal the population structure,mating mode,etc. in breeding programs.-
Keywords:
- Microsatellite /
- Fenneropenaeus chinensis /
- Kinship analysis
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